{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T19:35:44Z","timestamp":1789414544912,"version":"build-2803163510"},"reference-count":55,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,6,5]],"date-time":"2020-06-05T00:00:00Z","timestamp":1591315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2017M612439"],"award-info":[{"award-number":["2017M612439"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31170078"],"award-info":[{"award-number":["31170078"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2015CFA085"],"award-info":[{"award-number":["2015CFA085"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fundamental Research Funds for HUST","award":["2017KFYXJJ212"],"award-info":[{"award-number":["2017KFYXJJ212"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Candida tropicalis is a pathogenic fungus that can cause opportunistic infections in humans. The ability of Candida species to transition between yeast and filamentous growth forms is essential to their ability to undergo environmental adaptation and to maintain virulence. In other fungal species, such as Candida albicans, N-acetylglucosamine (GlcNAc) can induce filamentous growth, whereas it suppresses such growth in C. tropicalis. In the present study, we found that knocking out the GlcNA-specific transporter gene NGT1 was sufficient to enhance C. tropicalis filamentous growth on Lee\u2019s plus GlcNAc medium. This suggests that GlcNAc uptake into C. tropicalis cells is essential to the disruption of mycelial growth. As such, we further studied how GlcNAc catabolism-related genes were able to influence C. tropicalis filamentation. We found that HXK1 overexpression drove filamentous growth on Lee\u2019s media containing glucose and GlcNAc, whereas the deletion of the same gene disrupted this filamentous growth. Interestingly, the deletion of the DAC1 or NAG1 genes impaired C. tropicalis growth on Lee\u2019s plus GlcNAc plates. Overall, these results indicate that HXK1 can serve as a positive regulator of filamentous growth, with excess GlcNAc-6-PO4 accumulation being toxic to C. tropicalis. These findings may highlight novel therapeutic targets worthy of future investigation.<\/jats:p>","DOI":"10.3390\/ijms21114036","type":"journal-article","created":{"date-parts":[[2020,6,5]],"date-time":"2020-06-05T03:32:21Z","timestamp":1591327941000},"page":"4036","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["NGT1 Is Essential for N-Acetylglucosamine-Mediated Filamentous Growth Inhibition and HXK1 Functions as a Positive Regulator of Filamentous Growth in Candida tropicalis"],"prefix":"10.3390","volume":"21","author":[{"given":"Qiuyu","family":"Zhang","sequence":"first","affiliation":[{"name":"Key Laboratory of Molecular Biophysics, the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics, the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Yuan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics, the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghua","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics, the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuxia","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics, the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics, the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiming","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics, the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6643-597X","authenticated-orcid":false,"given":"Yunjun","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Biophysics, the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1111\/j.1574-6976.2011.00278.x","article-title":"Candida glabrata, Candida parapsilosis and Candida tropicalis: Biology, epidemiology, pathogenicity and antifungal resistance","volume":"36","author":"Silva","year":"2012","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.micpath.2018.09.026","article-title":"Emerging Candida species isolated from renal transplant recipients: Species distribution and susceptibility profiles","volume":"125","author":"Diba","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.tim.2004.05.008","article-title":"The distinct morphogenic states of Candida albicans","volume":"12","author":"Sudbery","year":"2004","journal-title":"Trends Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.3389\/fmicb.2016.02173","article-title":"Azole Antifungal Resistance in Candida albicans and Emerging Non-albicans Candida Species","volume":"7","author":"Whaley","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1111\/mmi.12310","article-title":"Bcr1 plays a central role in the regulation of opaque cell filamentation in Candida albicans","volume":"89","author":"Guan","year":"2013","journal-title":"Mol. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1002\/cld.491","article-title":"Hepatosplenic candidiasis","volume":"6","author":"Cornely","year":"2015","journal-title":"Clin. Liver Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"12916","DOI":"10.1038\/ncomms12916","article-title":"N-acetylglucosamine sensing by a GCN5-related N-acetyltransferase induces transcription via chromatin histone acetylation in fungi","volume":"7","author":"Su","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.semcdb.2018.01.008","article-title":"Organ-specific mechanisms linking innate and adaptive antifungal immunity","volume":"89","author":"Drummond","year":"2019","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/1469-0691.12539","article-title":"Global trends in the distribution of Candida species causing candidemia","volume":"20","author":"Guinea","year":"2014","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Kothalawala, M., Jayaweera, J.A.A.S., Arunan, S., and Jayathilake, A. (2019). The emergence of non-albicans candidemia and evaluation of HiChrome Candida differential agar and VITEK2 YST\u00ae platform for differentiation of Candida bloodstream isolates in teaching hospital Kandy, Sri Lanka. BMC Microbiol., 19.","DOI":"10.1186\/s12866-019-1518-3"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1053\/jhin.2001.1151","article-title":"Non-albicans Candida spp. causing fungaemia: Pathogenicity and antifungal resistance","volume":"50","author":"Krcmery","year":"2002","journal-title":"J. Hosp. Infect."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1086\/502294","article-title":"Susceptibilities of Candida species to amphotericin B and fluconazole: The emergence of fluconazole resistance in Candida tropicalis","volume":"25","author":"Yang","year":"2004","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1099\/jmm.0.013227-0","article-title":"Candida tropicalis: Its prevalence, pathogenicity and increasing resistance to fluconazole","volume":"59","author":"Kothavade","year":"2010","journal-title":"J. Med. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/S0092-8674(00)80358-X","article-title":"Nonfilamentous C. albicans mutants are avirulent","volume":"90","author":"Lo","year":"1997","journal-title":"Cell"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1128\/MMBR.00009-06","article-title":"Environmental sensing and signal transduction pathways regulating morphopathogenic determinants of Candida albicans","volume":"71","author":"Biswas","year":"2007","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/nrmicro2636","article-title":"Growth of Candida albicans hyphae","volume":"9","author":"Sudbery","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.chom.2019.02.008","article-title":"Candida albicans morphogenesis programs control the balance between gut commensalism and invasive infection","volume":"25","author":"Witchley","year":"2019","journal-title":"Cell Host Microbe"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1038\/250344a0","article-title":"Yeast-mycelial conversion induced by N-acetyl-D-glucosamine in Candida albicans","volume":"250","author":"Simonetti","year":"1974","journal-title":"Nature"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1016\/j.cub.2005.10.040","article-title":"Fungal adenylyl cyclase integrates CO2 sensing with cAMP signaling and virulence","volume":"15","author":"Klengel","year":"2005","journal-title":"Curr. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Tao, L., Zhang, Y., Fan, S., Nobile, C.J., Guan, G., and Huang, G. (2017). Integration of the tricarboxylic acid (TCA) cycle with cAMP signaling and Sfl2 pathways in the regulation of CO2 sensing and hyphal development in Candida albicans. PLoS Genet., 13.","DOI":"10.1371\/journal.pgen.1006949"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1038\/nature08064","article-title":"Evolution of pathogenicity and sexual reproduction in eight Candida genomes","volume":"459","author":"Butler","year":"2009","journal-title":"Nature"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.crvi.2011.05.008","article-title":"The genetic code of the fungal CTG clade","volume":"334","author":"Santos","year":"2011","journal-title":"Comptes Rendus Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.3389\/fmicb.2017.01927","article-title":"An update on Candida tropicalis based on basic and clinical approaches","volume":"8","author":"Chaves","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Lin, C.J., and Chen, Y.L. (2018). Conserved and divergent functions of the cAMP\/PKA signaling pathway in Candida albicans and Candida tropicalis. J. Fungi, 4.","DOI":"10.3390\/jof4020068"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1093\/femspd\/ftw101","article-title":"Candida tropicalis biofilm and human epithelium invasion is highly influenced by environmental pH","volume":"74","author":"Ferreira","year":"2016","journal-title":"Pathog. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"119","DOI":"10.4161\/viru.22913","article-title":"Candida albicans pathogenicity mechanisms","volume":"4","author":"Mayer","year":"2013","journal-title":"Virulence"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1038\/nrmicro2711","article-title":"Candida albicans morphogenesis and host defence: Discriminating invasion from colonization","volume":"10","author":"Gow","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"67","DOI":"10.2217\/fmb.09.113","article-title":"Molecular phylogenetics and epidemiology of Candida albicans","volume":"5","author":"Odds","year":"2010","journal-title":"Futur. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Burgain, A., Pic, \u00c9., Markey, L., Tebbji, F., Kumamoto, C.A., and Sellam, A. (2019). A novel genetic circuitry governing hypoxic metabolic flexibility, commensalism and virulence in the fungal pathogen Candida albicans. PLoS Pathog., 15.","DOI":"10.1101\/631960"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1002\/yea.1599","article-title":"Marine yeasts\u2014A review","volume":"25","author":"Kutty","year":"2008","journal-title":"Yeast"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/s11802-010-0139-0","article-title":"Distribution and diversity of Candida tropicalis strains in different marine environments","volume":"9","author":"Yan","year":"2010","journal-title":"J. Ocean Univ. China"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.sbi.2018.12.002","article-title":"Structure and function of extracellular O-GlcNAc","volume":"56","author":"Ogawa","year":"2019","journal-title":"Curr. Opin. Struct. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e01376-15","DOI":"10.1128\/mBio.01376-15","article-title":"N-acetylglucosamine-induced cell death in Candida albicans and its implications for adaptive mechanisms of nutrient sensing in yeasts","volume":"6","author":"Du","year":"2015","journal-title":"MBio"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1016\/j.ijbiomac.2019.06.123","article-title":"Non-catalytic conversion of chitin into Chromogen I in high-temperature water","volume":"136","author":"Osada","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Huang, G., Yi, S., Sahni, N., Daniels, K.J., Srikantha, T., and Soll, D.R. (2010). N-acetylglucosamine induces white to opaque switching, a mating prerequisite in Candida albicans. PLoS Pathog., 6.","DOI":"10.1371\/annotation\/ed4d1473-cf80-4e85-ad9d-7a390be260f6"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Gilmore, S.A., Naseem, S., Konopka, J.B., and Sil, A. (2013). N-acetylglucosamine (GlcNAc) triggers a rapid, temperature-responsive morphogenetic program in thermally dimorphic fungi. PLoS Genet., 9.","DOI":"10.1371\/journal.pgen.1003799"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6283","DOI":"10.1128\/MCB.17.11.6283","article-title":"HOY1, a homeo gene required for hyphal formation in Yarrowia lipolytica","volume":"17","author":"Dominguez","year":"1997","journal-title":"Mol. Cell Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1007\/s002840010333","article-title":"Factors affecting the morphogenetic switch in Yarrowia lipolytica","volume":"43","year":"2001","journal-title":"Curr. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1111\/mmi.13247","article-title":"Regulation of filamentation in the human fungal pathogen Candida tropicalis","volume":"99","author":"Zhang","year":"2015","journal-title":"Mol. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1091\/mbc.e06-10-0931","article-title":"Identification of an N-acetylglucosamine transporter that mediates hyphal induction in Candida albicans","volume":"18","author":"Alvarez","year":"2007","journal-title":"Mol. Biol. Cell"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"251","DOI":"10.4161\/viru.20010","article-title":"Regulation of phenotypic transitions in the fungal pathogen Candida albicans","volume":"3","author":"Huang","year":"2012","journal-title":"Virulence"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"14218","DOI":"10.1073\/pnas.250452997","article-title":"The inducible N-acetylglucosamine catabolic pathway gene cluster in Candida albicans: Discrete N-acetylglucosamine-inducible factors interact at the promoter of NAG1","volume":"97","author":"Kumar","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1111\/mmi.13256","article-title":"The N-acetylglucosamine catabolic gene cluster in Trichoderma reeseiis controlled by the Ndt80-like transcription factor RON1","volume":"99","author":"Kappel","year":"2015","journal-title":"Mol. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"28671","DOI":"10.1074\/jbc.M111.249854","article-title":"N-Acetylglucosamine (GlcNAc) induction of hyphal morphogenesis and transcriptional responses in Candida albicans are not dependent on its metabolism","volume":"286","author":"Naseem","year":"2011","journal-title":"J. Biol. Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1073\/pnas.0804061106","article-title":"Expression levels of a filament-specific transcriptional regulator are sufficient to determine Candida albicans morphology and virulence","volume":"106","author":"Carlisle","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1128\/EC.05085-11","article-title":"Coevolution of morphology and virulence in Candida Species","volume":"10","author":"Thompson","year":"2011","journal-title":"Eukaryot. Cell"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Naseem, S., and Konopka, J.B. (2015). N-acetylglucosamine regulates virulence properties in microbial pathogens. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1004947"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"167","DOI":"10.3389\/fmicb.2017.00556","article-title":"Whole genome-based amplified fragment length polymorphism analysis reveals genetic diversity in Candida africana","volume":"8","author":"Chowdhary","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2580","DOI":"10.1093\/emboj\/18.9.2580","article-title":"Phenotypic switching in Candida albicans is controlled by a SIR2 gene","volume":"18","author":"Johnson","year":"1999","journal-title":"EMBO J."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Rao, K.H., Ghosh, S., Natarajan, K., and Datta, A. (2013). N-Acetylglucosamine Kinase, HXK1 Is Involved in morphogenetic transition and metabolic gene expression in Candida albicans. PLoS ONE, 8.","DOI":"10.1371\/annotation\/c381f4a0-efa6-41e5-8bb5-172c714510b5"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.fgb.2016.05.001","article-title":"Role of the N-acetylglucosamine kinase (Hxk1) in the regulation of white-gray-opaque tristable phenotypic transitions in C","volume":"92","author":"Cao","year":"2016","journal-title":"albicans. Fungal Genet. Biol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.gene.2004.06.021","article-title":"The SAT1 flipper, an optimized tool for gene disruption in Candida albicans","volume":"341","author":"Vik","year":"2004","journal-title":"Gene"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Xie, J., Tao, L., Nobile, C.J., Tong, Y., Guan, G., Sun, Y., Cao, C., Hernday, A.D., Johnson, A.D., and Zhang, L. (2013). White-opaque switching in natural MTLa\/\u03b1 Isolates of Candida albicans: Evolutionary implications for roles in host adaptation, pathogenesis, and sex. PLoS Biol., 11.","DOI":"10.1371\/journal.pbio.1001525"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Porman, A.M., Hirakawa, M.P., Jones, S.K., Wang, N., and Bennett, R.J. (2013). MTL-independent phenotypic switching in Candida tropicalis and a dual role for Wor1 in regulating switching and filamentation. PLoS Genet., 9.","DOI":"10.1371\/journal.pgen.1003369"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Tao, L., Du, H., Guan, G., Dai, Y., Nobile, C.J., Liang, W., Cao, C., Zhang, Q., Zhong, J., and Huang, G. (2014). Discovery of a \u201cwhite-gray-opaque\u201d tristable phenotypic switching system in Candida albicans: Roles of non-genetic diversity in host adaptation. PLoS Biol., 12.","DOI":"10.1371\/journal.pbio.1001830"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/11\/4036\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:35:49Z","timestamp":1760175349000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/11\/4036"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,5]]},"references-count":55,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,6]]}},"alternative-id":["ijms21114036"],"URL":"https:\/\/doi.org\/10.3390\/ijms21114036","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,5]]}}}