{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T18:58:11Z","timestamp":1777575491937,"version":"3.51.4"},"reference-count":66,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T00:00:00Z","timestamp":1591660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["308995\/2012-0"],"award-info":[{"award-number":["308995\/2012-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Programa de Apoio \u00e0 P\u00f3s-Gradua\u00e7\u00e3o - CAPES-PROAP","award":["817757\/38860"],"award-info":[{"award-number":["817757\/38860"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>N-acetylcysteine (NAC) is a pharmacological alternative with great potential for reducing the deleterious effects of surgical procedures on patients with steatohepatitis. We evaluated the effect of NAC on hepatic ischemia\/reperfusion (I\/R) injury in C57BL\/6J mice, 8 weeks-old, weighing 25\u201330 g, with steatohepatitis induced by a methionine- and choline-deficient (MCD) diet. Groups: MCD group (steatohepatitis), MCD-I\/R group (steatohepatitis plus 30 min of 70% liver ischemia and 24 h of reperfusion), MCD-I\/R+NAC group (same as MCD-I\/R group plus 150 mg\/kg NAC 15 min before ischemia), and control group (normal AIN-93M diet). Liver enzymes and histopathology; nitrite and TBARS (thiobarbituric acid reactive substances) levels; pro-inflammatory cytokines; antioxidants enzymes; Nrf2 (nuclear factor erythroid-2-related factor 2) expression; and apoptosis were evaluated. In the group treated with NAC, reductions in inflammatory infiltration; AST (aspartate aminotransferase), nitrite, and TBARS levels; GPx (gutathione peroxidase) activity; cytokines synthesis; and number of apoptotic cells were observed while the GR (glutathione reductase) activity was increased. No differences were observed in Nfr2 expression or in SOD (superoxide dismutase), CAT (catalase), and GST (glutathione S-transferase) activities. Thus, it may be concluded that NAC exerts beneficial effects on mice livers with steatohepatitis submitted to I\/R by reducing oxidative stress, inflammatory response, and cell death.<\/jats:p>","DOI":"10.3390\/ijms21114106","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T05:16:14Z","timestamp":1591679774000},"page":"4106","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["N-Acetylcysteine Reduced Ischemia and Reperfusion Damage Associated with Steatohepatitis in Mice"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0905-2670","authenticated-orcid":false,"given":"Natalie","family":"Chaves Cayuela","sequence":"first","affiliation":[{"name":"Laboratory of Surgical Physiopathology\u2014LIM-08, Department of Surgery, Faculdade de Medicina da Universidade de S\u00e3o Paulo (FMUSP), 01246-903 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5556-8061","authenticated-orcid":false,"given":"Marcia","family":"Kiyomi Koike","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine\u2014LIM-51, FMUSP, 01246-903 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline","family":"Jacysyn","sequence":"additional","affiliation":[{"name":"Laboratory of Surgical Physiopathology\u2014LIM-08, Department of Surgery, Faculdade de Medicina da Universidade de S\u00e3o Paulo (FMUSP), 01246-903 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Rasslan","sequence":"additional","affiliation":[{"name":"Laboratory of Surgical Physiopathology\u2014LIM-08, Department of Surgery, Faculdade de Medicina da Universidade de S\u00e3o Paulo (FMUSP), 01246-903 S\u00e3o Paulo, SP, Brazil"},{"name":"General and Trauma Surgery Division, Department of Surgery, ICHC-FMUSP, 01246-903 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anderson","family":"Azevedo Cerqueira","sequence":"additional","affiliation":[{"name":"Departmento de Farmacologia, Instituto de Ci\u00eancas Biom\u00e9dicas, Universidade de S\u00e3o Paulo (USP), 05508-900 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2574-4490","authenticated-orcid":false,"given":"Soraia","family":"Pereira Costa","sequence":"additional","affiliation":[{"name":"Departmento de Farmacologia, Instituto de Ci\u00eancas Biom\u00e9dicas, Universidade de S\u00e3o Paulo (USP), 05508-900 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Pican\u00e7o Diniz-J\u00fanior","sequence":"additional","affiliation":[{"name":"Electron Microscopy Laboratory, Section of Hepatology, Instituto Evandro Chagas, 66093-020 Bel\u00e9m, PA, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8453-7184","authenticated-orcid":false,"given":"Edivaldo","family":"Massazo Utiyama","sequence":"additional","affiliation":[{"name":"Laboratory of Surgical Physiopathology\u2014LIM-08, Department of Surgery, Faculdade de Medicina da Universidade de S\u00e3o Paulo (FMUSP), 01246-903 S\u00e3o Paulo, SP, Brazil"},{"name":"General and Trauma Surgery Division, Department of Surgery, ICHC-FMUSP, 01246-903 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edna","family":"Frasson de Souza Montero","sequence":"additional","affiliation":[{"name":"Laboratory of Surgical Physiopathology\u2014LIM-08, Department of Surgery, Faculdade de Medicina da Universidade de S\u00e3o Paulo (FMUSP), 01246-903 S\u00e3o Paulo, SP, Brazil"},{"name":"General and Trauma Surgery Division, Department of Surgery, ICHC-FMUSP, 01246-903 S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,9]]},"reference":[{"key":"ref_1","first-page":"167","article-title":"Pathogenesis and Prevention of Hepatic Steatosis","volume":"11","author":"Nassir","year":"2015","journal-title":"Gastroenterol. 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