{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T12:00:39Z","timestamp":1792411239837,"version":"4.4.0"},"reference-count":45,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,6,14]],"date-time":"2020-06-14T00:00:00Z","timestamp":1592092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science Center","award":["2015\/19\/B\/NZ9\/01314"],"award-info":[{"award-number":["2015\/19\/B\/NZ9\/01314"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Both long-term undernutrition and overnutrition disturb metabolic balance, which is mediated partially by the action of two adipokines, leptin and resistin (RSTN). In this study, we manipulated the diet of ewes to produce either a thin (lean) or fat (fat) body condition and investigated how RSTN affects endocrine and metabolic status under different leptin concentrations. Twenty ewes were distributed into four groups (n = 5): the lean and fat groups were administered with saline (Lean and Fat), while the Lean-R (Lean-Resistin treated) and Fat-R (Fat-Resistin treated) groups received recombinant bovine resistin. Plasma was assayed for LH, FSH, PRL, RSTN, leptin, GH, glucose, insulin, total cholesterol, nonesterified fatty acid (NEFA), high-density lipoprotein (HDL)-cholesterol, low-density lipoprotein (LDL)-cholesterol and triglycerides. Expression levels of a suppressor of cytokine signaling (SOCS-3) and the long form of the leptin receptor (LRb) were determined in selected brain regions, such as the anterior pituitary, hypothalamic arcuate nucleus, preoptic area and ventro- and dorsomedial nuclei. The results indicate long-term alterations in body weight affect RSTN-mediated effects on metabolic and reproductive hormones concentrations and the expression of leptin signaling components: LRb and SOCS-3. This may be an adaptive mechanism to long-term changes in adiposity during the state of long-day leptin resistance.<\/jats:p>","DOI":"10.3390\/ijms21124238","type":"journal-article","created":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T03:17:32Z","timestamp":1592191052000},"page":"4238","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Changes in Expression of the Genes for the Leptin Signaling in Hypothalamic-Pituitary Selected Areas and Endocrine Responses to Long-Term Manipulation in Body Weight and Resistin in Ewes"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5562-7375","authenticated-orcid":false,"given":"Dorota Anna","family":"Zieba","sequence":"first","affiliation":[{"name":"Department of Animal Nutrition and Biotechnology, and Fisheries, Faculty of Animal Sciences, University of Agriculture in Krakow, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weronika","family":"Biernat","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Biotechnology, and Fisheries, Faculty of Animal Sciences, University of Agriculture in Krakow, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malgorzata","family":"Szczesna","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Biotechnology, and Fisheries, Faculty of Animal Sciences, University of Agriculture in Krakow, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Kirsz","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Biotechnology, and Fisheries, Faculty of Animal Sciences, University of Agriculture in Krakow, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3249-4538","authenticated-orcid":false,"given":"Justyna","family":"Bar\u0107","sequence":"additional","affiliation":[{"name":"Department of Animal Nutrition and Biotechnology, and Fisheries, Faculty of Animal Sciences, University of Agriculture in Krakow, 31-120 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2556-4983","authenticated-orcid":false,"given":"Tomasz","family":"Misztal","sequence":"additional","affiliation":[{"name":"Department of Animal Physiology, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 01-224 Jablonna, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1038\/372425a0","article-title":"Positional cloning of the mouse obese gene and its human homologue","volume":"372","author":"Zhang","year":"1994","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/0014-5793(96)00473-5","article-title":"Localization of leptin receptor mRNA and the long form splice variant (Ob-Rb) in mouse hypothalamus and adjacent brain regions by in situ hybridization","volume":"387","author":"Mercer","year":"1996","journal-title":"Febs Lett."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4880","DOI":"10.1210\/en.2004-0726","article-title":"Region-specific leptin resistance within the hypothalamus of diet-induced obese mice","volume":"145","author":"Flier","year":"2004","journal-title":"Endocrinology"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Zieba, D.A., Biernat, W., Szczesna, M., Kirsz, K., and Misztal, T. 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