{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T07:46:52Z","timestamp":1787730412130,"version":"build-2784847793"},"reference-count":53,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T00:00:00Z","timestamp":1592179200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>We report dual therapeutic effects of a synthetic heparin-binding peptide (HBP) corresponding to residues 15\u201324 of the heparin binding site in BMP4 in a collagen-induced rheumatic arthritis model (CIA) for the first time. The cell penetrating capacity of HBP led to improved cartilage recovery and anti-inflammatory effects via down-regulation of the iNOS-IFN\u03b3-IL6 signaling pathway in inflamed RAW264.7 cells. Both arthritis and paw swelling scores were significantly improved following HBP injection into CIA model mice. Anti-rheumatic effects were accelerated upon combined treatment with Enbrel\u00ae and HBP. Serum IFN\u03b3 and IL6 concentrations were markedly reduced following intraperitoneal HBP injection in CIA mice. The anti-rheumatic effects of HBP in mice were similar to those of Enbrel\u00ae. Furthermore, the combination of Enbrel\u00ae and HBP induced similar anti-rheumatic and anti-inflammatory effects as Enbrel\u00ae. We further investigated the effect of HBP on damaged chondrocytes in CIA mice. Regenerative capacity of HBP was confirmed based on increased expression of chondrocyte biomarker genes, including aggrecan, collagen type II and TNF\u03b1, in adult human knee chondrocytes. These findings collectively support the utility of our cell-permeable bifunctional HBP with anti-inflammatory and chondrogenic properties as a potential source of therapeutic agents for degenerative inflammatory diseases.<\/jats:p>","DOI":"10.3390\/ijms21124251","type":"journal-article","created":{"date-parts":[[2020,6,15]],"date-time":"2020-06-15T12:16:57Z","timestamp":1592223417000},"page":"4251","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A Synthetic Cell-Penetrating Heparin-Binding Peptide Derived from BMP4 with Anti-Inflammatory and Chondrogenic Functions for the Treatment of Arthritis"],"prefix":"10.3390","volume":"21","author":[{"given":"Da Hyeon","family":"Choi","sequence":"first","affiliation":[{"name":"School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju 28644, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongwoo","family":"Lee","sequence":"additional","affiliation":[{"name":"Central Research Institute, Nano Intelligent Biomedical Engineering Corporation (NIBEC), School of Dentistry, Seoul National University, Seoul 03080, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beom Soo","family":"Jo","sequence":"additional","affiliation":[{"name":"Central Research Institute, Nano Intelligent Biomedical Engineering Corporation (NIBEC), School of Dentistry, Seoul National University, Seoul 03080, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang-Sook","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Dental Regenerative Bioengineering and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyeong Eun","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju 28644, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju Kwang","family":"Choi","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju 28644, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon Jeong","family":"Park","sequence":"additional","affiliation":[{"name":"Central Research Institute, Nano Intelligent Biomedical Engineering Corporation (NIBEC), School of Dentistry, Seoul National University, Seoul 03080, Korea"},{"name":"Department of Dental Regenerative Bioengineering and Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jue-Yeon","family":"Lee","sequence":"additional","affiliation":[{"name":"Central Research Institute, Nano Intelligent Biomedical Engineering Corporation (NIBEC), School of Dentistry, Seoul National University, Seoul 03080, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon Shin","family":"Park","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Cheongju 28644, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kim, H.K., Cho, S.K., Kim, J.W., Jung, S.Y., Jang, E.J., Bae, S.C., Yoo, D.H., and Sung, Y.K. 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