{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T04:20:45Z","timestamp":1791519645721,"version":"4.3.4"},"reference-count":22,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2020,6,27]],"date-time":"2020-06-27T00:00:00Z","timestamp":1593216000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["108-2113-M-007-014"],"award-info":[{"award-number":["108-2113-M-007-014"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>There is an urgent and imminent need to develop new antimicrobials to fight against antibiotic-resistant bacterial and fungal strains. In this study, a checkerboard method was used to evaluate the synergistic effects of the antimicrobial peptide P-113 and its bulky non-nature amino acid substituted derivatives with vancomycin against vancomycin-resistant Enterococcus faecium, Staphylococcus aureus, and wild-type Escherichia coli. Boron-dipyrro-methene (BODIPY) labeled vancomycin was used to characterize the interactions between the peptides, vancomycin, and bacterial strains. Moreover, neutralization of antibiotic-induced releasing of lipopolysaccharide (LPS) from E. coli by the peptides was obtained. Among these peptides, Bip-P-113 demonstrated the best minimal inhibitory concentrations (MICs), antibiotics synergism, bacterial membrane permeabilization, and supernatant LPS neutralizing activities against the bacteria studied. These results could help in developing antimicrobial peptides that have synergistic activity with large size glycopeptides such as vancomycin in therapeutic applications.<\/jats:p>","DOI":"10.3390\/ijms21134578","type":"journal-article","created":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T05:28:56Z","timestamp":1593408536000},"page":"4578","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Antimicrobial Peptides Display Strong Synergy with Vancomycin Against Vancomycin-Resistant E. faecium, S. aureus, and Wild-Type E. coli"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3407-7076","authenticated-orcid":false,"given":"Chih-Lung","family":"Wu","sequence":"first","affiliation":[{"name":"Institute of Biotechnology and Department of Medical Science, National Tsing Hua University, Hsinchu 300, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3635-8448","authenticated-orcid":false,"given":"Ju-Yun","family":"Hsueh","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Department of Medical Science, National Tsing Hua University, Hsinchu 300, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bak-Sau","family":"Yip","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Department of Medical Science, National Tsing Hua University, Hsinchu 300, Taiwan"},{"name":"Department of Neurology, National Taiwan University Hospital Hsinchu Branch, Hsinchu 300, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Han","family":"Chih","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Department of Medical Science, National Tsing Hua University, Hsinchu 300, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuang-Li","family":"Peng","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Department of Medical Science, National Tsing Hua University, Hsinchu 300, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3331-691X","authenticated-orcid":false,"given":"Jya-Wei","family":"Cheng","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Department of Medical Science, National Tsing Hua University, Hsinchu 300, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1001\/jama.2016.11764","article-title":"Antimicrobial resistance","volume":"316","author":"Marston","year":"2016","journal-title":"JAMA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.diagmicrobio.2007.11.004","article-title":"In vitro activity of omiganan pentahydrochloride tested against vancomycin-tolerant -intermediate, and -resitant Staphylococcus aureus","volume":"60","author":"Fritsche","year":"2008","journal-title":"Diagn. 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