{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T17:55:44Z","timestamp":1792518944024,"version":"4.5.0"},"reference-count":62,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T00:00:00Z","timestamp":1593388800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["355803-2013"],"award-info":[{"award-number":["355803-2013"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["05420-2019"],"award-info":[{"award-number":["05420-2019"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["04275-2015"],"award-info":[{"award-number":["04275-2015"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Social interactions are typically impaired in neuropsychiatric disorders such as autism, for which the genetic underpinnings are very complex. Social interactions can be modeled by analysis of behaviors, including social spacing, sociability, and aggression, in simpler organisms such as Drosophila melanogaster. Here, we examined the effects of mutants of the autism-related gene neuroligin 3 (nlg3) on fly social and non-social behaviors. Startled-induced negative geotaxis is affected by a loss of function nlg3 mutation. Social space and aggression are also altered in a sex- and social-experience-specific manner in nlg3 mutant flies. In light of the conserved roles that neuroligins play in social behavior, our results offer insight into the regulation of social behavior in other organisms, including humans.<\/jats:p>","DOI":"10.3390\/ijms21134601","type":"journal-article","created":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T05:28:56Z","timestamp":1593408536000},"page":"4601","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Abnormal Social Interactions in a Drosophila Mutant of an Autism Candidate Gene: Neuroligin 3"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0637-2871","authenticated-orcid":false,"given":"Ryley T.","family":"Yost","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science, Western University, London, ON N6A 5B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Wesley","family":"Robinson","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Western University, London, ON N6A 5B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carling M.","family":"Baxter","sequence":"additional","affiliation":[{"name":"Animal Behaviour Group, Department of Psychology, Neuroscience and Behaviour (PNB) McMaster University, Hamilton, ON L8S 4K1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew M.","family":"Scott","sequence":"additional","affiliation":[{"name":"Animal Behaviour Group, Department of Psychology, Neuroscience and Behaviour (PNB) McMaster University, Hamilton, ON L8S 4K1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2406-8910","authenticated-orcid":false,"given":"Liam P.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Western University, London, ON N6A 5B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Sol","family":"Aletta","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Western University, London, ON N6A 5B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramtin","family":"Hakimjavadi","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Western University, London, ON N6A 5B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asad","family":"Lone","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Western University, London, ON N6A 5B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert C.","family":"Cumming","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Western University, London, ON N6A 5B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reuven","family":"Dukas","sequence":"additional","affiliation":[{"name":"Animal Behaviour Group, Department of Psychology, Neuroscience and Behaviour (PNB) McMaster University, Hamilton, ON L8S 4K1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Mozer","sequence":"additional","affiliation":[{"name":"Office of Research Integrity, Office of the Assistant Secretary for Health, Rockville, MD 20889, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6509-4541","authenticated-orcid":false,"given":"Anne F.","family":"Simon","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Western University, London, ON N6A 5B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.cobeha.2015.11.007","article-title":"Proximate pathways underlying social behavior","volume":"6","author":"Rubenstein","year":"2015","journal-title":"Curr. Opin. Behav. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e12598","DOI":"10.1111\/gbb.12598","article-title":"Social behavior and aging: A fly model","volume":"19","author":"Yost","year":"2020","journal-title":"Genes Brain Behav."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1016\/j.bbr.2013.06.020","article-title":"Monogenic heritable autism gene neuroligin impacts Drosophila social behavior","volume":"252","author":"Hahn","year":"2013","journal-title":"Behav. Brain Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1097\/GIM.0b013e3181ff67ba","article-title":"Autism spectrum disorders--a genetics review","volume":"13","author":"Miles","year":"2011","journal-title":"Genet. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1016\/j.neuron.2010.03.007","article-title":"Social interactions in \u201csimple\u201d model systems","volume":"65","author":"Sokolowski","year":"2010","journal-title":"Neuron"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1242\/jeb.141457","article-title":"The neurogenetics of group behavior in Drosophila melanogaster","volume":"220","author":"Ramdya","year":"2017","journal-title":"J. Exp. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1038\/35098592","article-title":"Drosophila: genetics meets behavior","volume":"2","author":"Sokolowski","year":"2001","journal-title":"Nat. Rev. Genet."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.neuron.2018.02.026","article-title":"Neural circuit mechanisms of social behavior","volume":"98","author":"Chen","year":"2018","journal-title":"Neuron"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.conb.2016.04.007","article-title":"Neural control of aggression in Drosophila","volume":"38","author":"Hoopfer","year":"2016","journal-title":"Curr. Opin. Neurobiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1038\/nrn3567","article-title":"Genes and circuits of courtship behavior in Drosophila males","volume":"14","author":"Yamamoto","year":"2013","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1007\/s10519-018-9901-7","article-title":"Sociability in fruit flies: Genetic variation, heritability and plasticity","volume":"48","author":"Scott","year":"2018","journal-title":"Behav. Genet."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Yost, R.T., Liang, E., Stewart, M.P., Chui, S., Greco, A.F., Long, S.Q., McDonald, I.S., McNeil, J.N., and Simon, A.F. (2019). Is Drosophila melanogaster stress odorant (dSO) really an alarm pheromone?. bioRxiv, 534719.","DOI":"10.1101\/534719"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.cois.2019.09.001","article-title":"What can a non-eusocial insect tell us about the neural basis of group behavior?","volume":"36","author":"Ferreira","year":"2019","journal-title":"Curr. Opin. Insect Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1111\/j.1601-183X.2011.00740.x","article-title":"A simple assay to study social behavior in Drosophila: measurement of social space within a group","volume":"11","author":"Simon","year":"2012","journal-title":"Genes Brain Behav."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1016\/j.celrep.2018.03.068","article-title":"A genetic toolkit for dissecting dopamine circuit function in Drosophila","volume":"23","author":"Xie","year":"2018","journal-title":"Cell Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"20170369","DOI":"10.1098\/rsbl.2017.0369","article-title":"Modulation of social space by dopamine in Drosophila melanogaster, but no effect on the avoidance of the Drosophila stress odorant","volume":"13","author":"Fernandez","year":"2017","journal-title":"Biol. Lett."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/01677063.2015.1064916","article-title":"Drosophila mutants of the autism candidate gene neurobeachin (rugose) exhibit neuro-developmental disorders, aberrant synaptic properties, altered locomotion, and impaired adult social behavior and activity patterns","volume":"29","author":"Wise","year":"2015","journal-title":"J. Neurogenet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"216","DOI":"10.4161\/fly.4.3.12280","article-title":"An assay for social interaction in Drosophila fragile X mutants","volume":"4","author":"Bolduc","year":"2010","journal-title":"Fly"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e0211652","DOI":"10.1371\/journal.pone.0211652","article-title":"Conserved regulation of neurodevelopmental processes and behavior by FoxP in Drosophila","volume":"14","author":"Eidhof","year":"2019","journal-title":"PLoS ONE"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fpsyt.2017.00113","article-title":"Neuroligins Nlg2 and Nlg4 affect social behavior in Drosophila melanogaster","volume":"8","author":"Corthals","year":"2017","journal-title":"Front. Psychiatry"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.bbr.2015.02.001","article-title":"Effect of bisphenol A on Drosophila melanogaster behavior--a new model for the studies on neurodevelopmental disorders","volume":"284","author":"Kaur","year":"2015","journal-title":"Behav. Brain Res."},{"key":"ref_22","first-page":"1","article-title":"Progeny of old parents have increased social space in Drosophila melanogaster","volume":"8","author":"Long","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/j.neurobiolaging.2013.09.032","article-title":"Role for the neurexin-neuroligin complex in Alzheimer\u2018s disease","volume":"35","author":"Sindi","year":"2014","journal-title":"Neurobiol. Aging"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1007\/s12035-011-8213-1","article-title":"Neurexins and neuroligins: recent insights from invertebrates","volume":"44","author":"Knight","year":"2011","journal-title":"Mol. Neurobiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1523\/JNEUROSCI.3854-10.2011","article-title":"Neuroligin 2 is required for synapse development and function at the Drosophila neuromuscular junction","volume":"31","author":"Sun","year":"2011","journal-title":"J. Neurosci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"16018","DOI":"10.1523\/JNEUROSCI.1685-12.2012","article-title":"Drosophila neuroligin 2 is required presynaptically and postsynaptically for proper synaptic differentiation and synaptic transmission","volume":"32","author":"Chen","year":"2012","journal-title":"J. Neurosci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.mcn.2012.08.010","article-title":"Drosophila neuroligin 1 regulates synaptic growth and function in response to activity and phosphoinositide-3-kinase","volume":"51","author":"Mozer","year":"2012","journal-title":"Mol. Cell Neurosci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"31867","DOI":"10.1074\/jbc.M114.574897","article-title":"Drosophila neuroligin3 regulates neuromuscular junction development and synaptic differentiation","volume":"289","author":"Xing","year":"2014","journal-title":"J. Biol. Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"D759","DOI":"10.1093\/nar\/gky1003","article-title":"FlyBase 2.0: the next generation","volume":"47","author":"Thurmond","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1038\/459927a","article-title":"Unlocking the secrets of the genome","volume":"459","author":"Celniker","year":"2009","journal-title":"Nature"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1523\/JNEUROSCI.0819-13.2013","article-title":"Drosophila neuroligin 4 regulates sleep through modulating GABA transmission","volume":"33","author":"Li","year":"2013","journal-title":"J. Neurosci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1016\/j.pharep.2014.04.011","article-title":"Neuroligins, synapse balance and neuropsychiatric disorders","volume":"66","author":"Mackowiak","year":"2014","journal-title":"Pharm. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1016\/j.bbagen.2017.10.021","article-title":"Proteolytic maturation of Drosophila Neuroligin 3 by tumor necrosis factor alpha-converting enzyme in the nervous system","volume":"1862","author":"Wu","year":"2018","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1534\/genetics.110.122754","article-title":"Socially-responsive gene expression in male Drosophila melanogaster is influenced by the sex of the interacting partner","volume":"187","author":"Ellis","year":"2011","journal-title":"Genetics"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"McNeil, A.R., Jolley, S.N., Akinleye, A.A., Nurilov, M., Rouzyi, Z., Milunovich, A.J., Chambers, M.C., and Simon, A.F. (2015). Conditions affecting social space in Drosophila melanogaster. J. Vis. Exp., e53242.","DOI":"10.3791\/53242-v"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.anbehav.2016.10.022","article-title":"Life history of aggression: effects of age and sexual experience on male aggression towards males and females","volume":"123","author":"Baxter","year":"2017","journal-title":"Anim. Behav."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Fernandez, R.W., Nurilov, M., Feliciano, O., McDonald, I.S., and Simon, A.F. (2014). Straightforward assay for quantification of social avoidance in Drosophila melanogaster. J. Vis. Exp., e52011.","DOI":"10.3791\/52011"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1093\/genetics\/151.2.725","article-title":"The gene search system: A method for efficient detection and rapid molecular edentification of genes in Drosophila melanogaster","volume":"151","author":"Toba","year":"1999","journal-title":"Genetics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.neuron.2018.09.040","article-title":"Towards an understanding of synapse formation","volume":"100","author":"Sudhof","year":"2018","journal-title":"Neuron"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4889","DOI":"10.1523\/JNEUROSCI.0468-04.2004","article-title":"The Arg451Cys-neuroligin-3 mutation associated with autism reveals a defect in protein processing","volume":"24","author":"Comoletti","year":"2004","journal-title":"J. Neurosci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"13764","DOI":"10.1073\/pnas.1111093108","article-title":"Autism-linked neuroligin-3 R451C mutation differentially alters hippocampal and cortical synaptic function","volume":"108","author":"Etherton","year":"2011","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1093\/beheco\/arv225","article-title":"Social behavior and activity are decoupled in larval and adult fruit flies","volume":"27","author":"Anderson","year":"2016","journal-title":"Behav. Ecol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13229-015-0055-7","article-title":"A neuroligin-3 mutation implicated in autism causes abnormal aggression and increases repetitive behavior in mice","volume":"6","author":"Burrows","year":"2015","journal-title":"Mol. Autism"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1002\/aur.22","article-title":"Minimal aberrant behavioral phenotypes of neuroligin-3 R451C knockin mice","volume":"1","author":"Chadman","year":"2008","journal-title":"Autism Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/j.cell.2005.04.027","article-title":"fruitless splicing specifies male courtship behavior in Drosophila","volume":"121","author":"Demir","year":"2005","journal-title":"Cell"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1016\/j.cub.2008.07.089","article-title":"Social experience modifies pheromone expression and mating behavior in male Drosophila melanogaster","volume":"18","author":"Krupp","year":"2008","journal-title":"Curr. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"5657","DOI":"10.1073\/pnas.0801327105","article-title":"A common genetic target for environmental and heritable influences on aggressiveness in Drosophila","volume":"105","author":"Wang","year":"2008","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1046\/j.1365-3032.2002.00262.x","article-title":"Aggressive behaviors of female Drosophila melanogaster are influenced by their social experience and food resources","volume":"27","author":"Ueda","year":"2002","journal-title":"Physiol. Entomol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"378","DOI":"10.3109\/01677060903063026","article-title":"Effects of social isolation on neuromuscular excitability and aggressive behaviors in Drosophila: altered responses by Hk and gsts1, two mutations implicated in redox regulation","volume":"23","author":"Ueda","year":"2009","journal-title":"J. Neurogenet."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1016\/j.cub.2008.07.088","article-title":"Social context influences chemical communication in D. melanogaster males","volume":"18","author":"Kent","year":"2008","journal-title":"Curr. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1126\/science.1130408","article-title":"Waking experience affects sleep need in Drosophila","volume":"313","author":"Donlea","year":"2006","journal-title":"Science"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1016\/j.cub.2009.08.017","article-title":"Social facilitation of long-lasting memory retrieval in Drosophila","volume":"19","author":"Chabaud","year":"2009","journal-title":"Curr. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1038\/s41559-020-1179-5","article-title":"The Y chromosome may contribute to sex-specific ageing in Drosophila","volume":"4","author":"Brown","year":"2020","journal-title":"Nat. Ecol. Evol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/ng1136","article-title":"Mutations of the X-linked genes encoding neuroligins NLGN3 and NLGN4 are associated with autism","volume":"34","author":"Jamain","year":"2003","journal-title":"Nat. Genet."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1038\/ng1314","article-title":"A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac","volume":"36","author":"Thibault","year":"2004","journal-title":"Nat. Genet."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Posch, A., Kohn, J., Oh, K., Hammond, M., and Liu, N. (2013). V3 stain-free workflow for a practical, convenient, and reliable total protein loading control in western blotting. J. Vis. Exp., 50948.","DOI":"10.3791\/50948-v"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.jneumeth.2008.05.003","article-title":"The use of total protein stains as loading controls: an alternative to high-abundance single-protein controls in semi-quantitative immunoblotting","volume":"172","author":"Aldridge","year":"2008","journal-title":"J. Neurosci. Methods"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nature Methods"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"5664","DOI":"10.1073\/pnas.082102599","article-title":"Fighting fruit flies: a model system for the study of aggression","volume":"99","author":"Chen","year":"2002","journal-title":"Proc. Natl. Acad. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s003590050307","article-title":"Temporal pattern of locomotor activity in Drosophila melanogaster","volume":"184","author":"Martin","year":"1999","journal-title":"J. Comp. Physiol. A"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1111\/2041-210X.12584","article-title":"BORIS: A free, versatile open source event logging software for video\/audio coding and live observations","volume":"7","author":"Friard","year":"2016","journal-title":"Methods Ecol. Evol."},{"key":"ref_62","first-page":"1112","article-title":"Behavioural mutants of Drosophila isolated by countercurrent distribution","volume":"58","author":"Benzer","year":"1967","journal-title":"Genetics"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/13\/4601\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:44:14Z","timestamp":1760175854000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/13\/4601"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,29]]},"references-count":62,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2020,7]]}},"alternative-id":["ijms21134601"],"URL":"https:\/\/doi.org\/10.3390\/ijms21134601","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,29]]}}}