{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T03:53:17Z","timestamp":1784001197533,"version":"3.55.0"},"reference-count":44,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T00:00:00Z","timestamp":1594166400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Currently, endometrial carcinoma (EC) is the most common genital cancer in high-income countries. Some types of endometrial hyperplasia (EH) may be progressing to this malignancy. The diagnosis of EC and EH is based on time consuming histopathology evaluation, which is subjective and causes discrepancies in reassessment. Therefore, there is a need to create methods of objective evaluation allowing the diagnosis of early changes. The study aimed to simultaneously asses Fourier Transform Infrared (FTIR) and Raman spectroscopy combined with multidimensional analysis to identify the tissues of endometrial cancer, atypical hyperplasia and the normal control group, and differentiate them. The results of FTIR and Raman spectroscopy revealed quantitative and qualitative changes in the nucleic acid and protein in the groups of cancer and atypical hyperplasia, in comparison with the control group. Changes in the lipid region were also observed in Raman spectra. Pearson correlation coefficient demonstrated a statistically significant correlation between Raman spectra for the cancer and atypical hyperplasia groups (0.747, p &lt; 0.05) and for atypical hyperplasia and the controls (0.507, p &lt; 0.05), while FTIR spectra demonstrated a statistically significant positive correlation for the same group as in Raman data and for the control and cancer groups (0.966, p &lt; 0.05). To summarize, the method of spectroscopy enables differentiation of atypical hyperplasia and endometrial cancer tissues from the physiological endometrial tissue.<\/jats:p>","DOI":"10.3390\/ijms21144828","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T11:47:46Z","timestamp":1594208866000},"page":"4828","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Simultaneous FTIR and Raman Spectroscopy in Endometrial Atypical Hyperplasia and Cancer"],"prefix":"10.3390","volume":"21","author":[{"given":"Edyta","family":"Barnas","sequence":"first","affiliation":[{"name":"Institute of Health Sciences, College of Medical Sciences, University of Rzeszow, Kopisto 2a, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Skret-Magierlo","sequence":"additional","affiliation":[{"name":"Medical College of Rzeszow University, Institute of Medical Sciences, Kopisto 2a, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Skret","sequence":"additional","affiliation":[{"name":"Medical College of Rzeszow University, Institute of Medical Sciences, Kopisto 2a, 35-959 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Kaznowska","sequence":"additional","affiliation":[{"name":"Department of Pathomorphology, Chair of Morphological Sciences, College of Medical Sciences, University of Rzeszow, Kopisto 2a, 35-959 Rzeszow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0168-1701","authenticated-orcid":false,"given":"Joanna","family":"Depciuch","sequence":"additional","affiliation":[{"name":"Institute of Nuclear Physics, Polish Academy of Science, Radzikowskiego 152, 31-342 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamil","family":"Szmuc","sequence":"additional","affiliation":[{"name":"Institute of Physics, College of Natural Sciences, University of Rzeszow, - St. Pigonia 1, 35-310 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kornelia","family":"\u0141ach","sequence":"additional","affiliation":[{"name":"Clinic of Paediatric Oncology and Haematology, College of Medical Sciences, University of Rzeszow, Kopisto 2a, 35-959 Rzeszow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izabela","family":"Krawczyk-Mar\u0107","sequence":"additional","affiliation":[{"name":"Department of Human Histology, Chair of Morphological Sciences, College of Medical Sciences, University of Rzeszow, Kopisto 2a, 35-959 Rzeszow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5657-5354","authenticated-orcid":false,"given":"Jozef","family":"Cebulski","sequence":"additional","affiliation":[{"name":"Institute of Physics, College of Natural Sciences, University of Rzeszow, - St. Pigonia 1, 35-310 Rzesz\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"E359","DOI":"10.1002\/ijc.29210","article-title":"Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012","volume":"136","author":"Ferlay","year":"2015","journal-title":"Int. J. Cancer"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1159\/000321390","article-title":"Comparison of the Efficacy of Three Progestins in the Treatment of Simple Endometrial Hyperplasia without Atypia","volume":"72","author":"Ozdegirmenci","year":"2011","journal-title":"Gynecol. Obstet. Investig."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Collins, F., MacPherson, S., Brown, P., Bombail, V., Williams, A.R.W., Anderson, R.A., Jabbours, H.N., and Saunders, P.T.K. (2019). Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha. BMC Cancer, 9.","DOI":"10.1186\/1471-2407-9-330"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1111\/his.12053","article-title":"Molecular pathology of endometrial carcinoma","volume":"62","author":"Prat","year":"2013","journal-title":"Histopathology"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ellenson, L.H., Ronnett, B.M., and Kurman, R.J. (2011). Precursor Lesions of Endometrial Carcinoma. Blaustein\u2019s Pathology of the Female Genital Tract, Springer.","DOI":"10.1007\/978-1-4419-0489-8_8"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1002\/1097-0142(19850715)56:2<403::AID-CNCR2820560233>3.0.CO;2-X","article-title":"The behavior of endometrial hyperplasia. A long-term study of \u201cuntreated\u201d hyperplasia in 170 patients","volume":"56","author":"Kurman","year":"1985","journal-title":"Cancer"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/sj.bjc.6604102","article-title":"Endometrial carcinoma risk among women diagnosed with endometrial hyperplasia: The 34-year experience in a large health plan","volume":"98","author":"Lacey","year":"2008","journal-title":"Br. J. Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1097\/00004347-199701000-00006","article-title":"Comparison of the Reproducibility of the WHO Classifications of 1975 and 1994 of Endometrial Hyperplasia","volume":"16","author":"Skov","year":"1997","journal-title":"Int. J. Gynecol. Pathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1097\/00000478-199808000-00012","article-title":"Reproducibility of the Diagnosis of Endometrial Hyperplasia, Atypical Hyperplasia, and Well-Differentiated Carcinoma","volume":"22","author":"Kendall","year":"1998","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1002\/cncr.21650","article-title":"Concurrent endometrial carcinoma in women with a biopsy diagnosis of atypical endometrial hyperplasia","volume":"106","author":"Trimble","year":"2006","journal-title":"Cancer"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1529\/biophysj.105.066761","article-title":"Micro-Raman Spectroscopy Detects Individual Neoplastic and Normal Hematopoietic Cells","volume":"90","author":"Chan","year":"2006","journal-title":"Biophys. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.jpba.2017.09.016","article-title":"Qualitative and quantitative changes in phospholipids and proteins investigated by spectroscopic techniques in olfactory bulbectomy animal depression model","volume":"148","author":"Depciuch","year":"2018","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1177\/0003702815620127","article-title":"Application of Raman Spectroscopy and Infrared Spectroscopy in the Identification of Breast Cancer","volume":"70","author":"Depciuch","year":"2016","journal-title":"Appl. Spectrosc."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.infrared.2018.01.021","article-title":"Application of infrared spectroscopy for the identification of squamous cell carcinoma (lung cancer). Preliminary study","volume":"89","author":"Kaznowska","year":"2018","journal-title":"Infrared Phys. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"15081","DOI":"10.1038\/s41598-018-33470-3","article-title":"Distinguishing Ewing sarcoma and osteomyelitis using FTIR spectroscopy","volume":"8","author":"Chaber","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.jpba.2019.03.061","article-title":"Spectroscopic identification of benign (follicular adenoma) and cancerous lesions (follicular thyroid carcinoma) in thyroid tissues","volume":"170","author":"Depciuch","year":"2019","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"117526","DOI":"10.1016\/j.saa.2019.117526","article-title":"Raman and FTIR spectroscopy in determining the chemical changes in healthy brain tissues and glioblastoma tumor tissues","volume":"225","author":"Depciuch","year":"2020","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1007\/s10555-018-9770-9","article-title":"Applications of Raman spectroscopy in cancer diagnosis","volume":"37","author":"Auner","year":"2018","journal-title":"Cancer Metastasis Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4541","DOI":"10.1007\/s00216-018-1111-x","article-title":"Synchrotron- and focal plane array-based Fourier-transform infrared spectroscopy differentiates the basalis and functionalis epithelial endometrial regions and identifies putative stem cell regions of human endometrial glands","volume":"410","author":"Theophilou","year":"2018","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_20","first-page":"61","article-title":"Endometriosis and the neoplastic process","volume":"10","author":"Sznurkowski","year":"2012","journal-title":"Curr. Gynecol. Oncol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"19795","DOI":"10.1038\/s41598-019-56308-y","article-title":"Raman spectroscopy as a non-invasive diagnostic technique for endometriosis","volume":"9","author":"Parlatan","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1002\/(SICI)1520-6343(1996)2:3<155::AID-BSPY2>3.0.CO;2-7","article-title":"Pressure-tuning fourier transform infrared spectroscopic study of carcinogenesis in human endometrium","volume":"2","author":"Fung","year":"1996","journal-title":"Biospectroscopy"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.canlet.2008.09.018","article-title":"Derivation of a subtype-specific biochemical signature of endometrial carcinoma using synchrotron-based Fourier-transform infrared microspectroscopy","volume":"274","author":"Kelly","year":"2009","journal-title":"Cancer Lett."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1038\/sj.bjc.6606094","article-title":"Infrared spectroscopy with multivariate analysis to interrogate endometrial tissue: A novel and objective diagnostic approach","volume":"104","author":"Taylor","year":"2011","journal-title":"Br. J. Cancer"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1016\/j.polymer.2004.12.008","article-title":"Spectroscopic and structural characterization of low molecular weight fractions of polyaniline","volume":"46","author":"Laska","year":"2005","journal-title":"Polymer"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/s00216-005-0097-3","article-title":"Surface-enhanced Raman scattering detection of lysophosphatidic acid","volume":"383","author":"Seballos","year":"2005","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1038\/sj.bjc.6603102","article-title":"Raman spectroscopy: Elucidation of biochemical changes in carcinogenesis of oesophagus","volume":"94","author":"Shetty","year":"2006","journal-title":"Br. J. Cancer"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1080\/05704920701551530","article-title":"Raman Spectroscopy of Biological Tissues","volume":"42","author":"Movasaghi","year":"2007","journal-title":"Appl. Spectrosc. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1002\/jbio.201500248","article-title":"A comparative evaluation of diffuse reflectance and Raman spectroscopy in the detection of cervical cancer","volume":"10","author":"Shaikh","year":"2016","journal-title":"J. Biophotonics"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/S0022-2860(01)00527-0","article-title":"Analysis of human cancer prostate tissues using FTIR microscopy and SXIXE techniques","volume":"565\u2013566","author":"Paluszkiewicz","year":"2001","journal-title":"J. Mol. Struct."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1002\/bip.20154","article-title":"Inflammatory bowel diseases as an intermediate stage between normal and cancer: A FTIR-microspectroscopy approach","volume":"75","author":"Argov","year":"2004","journal-title":"Biopolymers"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0924-2031(01)00149-7","article-title":"Identification of tumor tissue by FTIR spectroscopy in combination with positron emission tomography","volume":"28","author":"Richter","year":"2002","journal-title":"Vib. Spectrosc."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Gremlich, H.U., and Yan, B. (2001). FT-infrared and FT-Raman spectroscopy in biomedical research. Infrared and Raman Spectroscopy of Biological Materials, Marcel Dekker.","DOI":"10.1201\/b16936"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"10988","DOI":"10.1073\/pnas.88.24.10988","article-title":"Infrared spectroscopy of exfoliated human cervical cells: Evidence of extensive structural changes during carcinogenesis","volume":"88","author":"Wong","year":"1991","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.cdp.2003.11.004","article-title":"Discrimination between normal and malignant human gastric tissues by Fourier transform infrared spectroscopy","volume":"28","author":"Fujioka","year":"2004","journal-title":"Cancer Detect. Prev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.molstruc.2007.08.040","article-title":"FT-IR microimaging spectroscopy: A comparison between healthy and neoplastic human colon tissues","volume":"881","author":"Conti","year":"2008","journal-title":"J. Mol. Struct."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/j.bbabio.2007.06.004","article-title":"Infrared spectroscopy of proteins","volume":"1767","author":"Barth","year":"2007","journal-title":"Biochim. Biophys. Acta Bioenerg."},{"key":"ref_38","first-page":"232","article-title":"New concepts for an old problem: The diagnosis of endometrial hyperplasia","volume":"23","author":"Sanderson","year":"2017","journal-title":"Hum. Reprod. Updat."},{"key":"ref_39","unstructured":"Herzbeg, G. (1945). Infrared and Raman Spectra, Van Nostrand."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Woodward, L.A. (1972). Introduction to the Theory of Molecular Vibrations and Vibrational Spectroscopy, Clarendon Press.","DOI":"10.1016\/0022-2860(72)85202-5"},{"key":"ref_41","first-page":"6799","article-title":"Cancer Incidence in Patients with Atypical Endometrial Hyperplasia Managed by Primary Hysterectomy or Fertility-sparing Treatment","volume":"35","author":"Gonthier","year":"2015","journal-title":"Anticancer Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.gmit.2016.02.001","article-title":"Risk of endometrial cancer in patients with a preoperative diagnosis of atypical endometrial hyperplasia treated with total laparoscopic hysterectomy","volume":"5","author":"Oda","year":"2016","journal-title":"Gynecol. Minim. Invasive Ther."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"828","DOI":"10.17772\/gp\/1908","article-title":"Evaluation of genomic imbalance in endometrial hyperplasia and carcinoma","volume":"85","author":"Bednarek","year":"2014","journal-title":"Ginekol. Polska"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Njoku, K., Chiasserini, D., Whetton, A.D., and Crosbie, E.J. (2019). Proteomic Biomarkers for the Detection of Endometrial Cancer. Cancers, 11.","DOI":"10.3390\/cancers11101572"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/14\/4828\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:48:55Z","timestamp":1760176135000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/14\/4828"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,8]]},"references-count":44,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2020,7]]}},"alternative-id":["ijms21144828"],"URL":"https:\/\/doi.org\/10.3390\/ijms21144828","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,8]]}}}