{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T10:13:21Z","timestamp":1771668801112,"version":"3.50.1"},"reference-count":32,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2020,7,12]],"date-time":"2020-07-12T00:00:00Z","timestamp":1594512000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Polish Cardiac Society","award":["2012 annual prize and scientific grant of the Polish Cardiac Society"],"award-info":[{"award-number":["2012 annual prize and scientific grant of the Polish Cardiac Society"]}]},{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["DEC-2016\/21\/B\/ST7\/02241"],"award-info":[{"award-number":["DEC-2016\/21\/B\/ST7\/02241"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2015\/19\/D\/NZ1\/03443"],"award-info":[{"award-number":["2015\/19\/D\/NZ1\/03443"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Perturbations of glycosaminoglycan metabolism lead to mucopolysaccharidoses (MPS)\u2014lysosomal storage diseases. One type of MPS (type VI) is associated with a deficiency of arylsulfatase B (ARSB), for which we previously established a cellular model using pulmonary artery endothelial cells with a silenced ARSB gene. Here, we explored the effects of silencing the ARSB gene on the growth of human pulmonary artery smooth muscle cells in the presence of different concentrations of dermatan sulfate (DS). The viability of pulmonary artery smooth muscle cells with a silenced ARSB gene was stimulated by the dermatan sulfate. In contrast, the growth of pulmonary artery endothelial cells was not affected. As shown by microarray analysis, the expression of the arylsulfatase G (ARSG) in pulmonary artery smooth muscle cells increased after silencing the arylsulfatase B gene, but the expression of genes encoding other enzymes involved in the degradation of dermatan sulfate did not. The active site of arylsulfatase G closely resembles that of arylsulfatase B, as shown by molecular modeling. Together, these results lead us to propose that arylsulfatase G can take part in DS degradation; therefore, it can affect the functioning of the cells with a silenced arylsulfatase B gene.<\/jats:p>","DOI":"10.3390\/ijms21144913","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T09:30:49Z","timestamp":1594719049000},"page":"4913","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A Possible Role for Arylsulfatase G in Dermatan Sulfate Metabolism"],"prefix":"10.3390","volume":"21","author":[{"given":"Aleksandra","family":"Poterala-Hejmo","sequence":"first","affiliation":[{"name":"Department of Systems Biology and Engineering, Silesian University of Technology, 44-100 Gliwice, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8266-2221","authenticated-orcid":false,"given":"Adam","family":"Golda","sequence":"additional","affiliation":[{"name":"Department of Cardiology, 4th Municipal Hospital, 44-100 Gliwice, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9741-2137","authenticated-orcid":false,"given":"Marcin","family":"Pacholczyk","sequence":"additional","affiliation":[{"name":"Department of Systems Biology and Engineering, Silesian University of Technology, 44-100 Gliwice, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1812-5496","authenticated-orcid":false,"given":"Sebastian","family":"Student","sequence":"additional","affiliation":[{"name":"Department of Systems Biology and Engineering, Silesian University of Technology, 44-100 Gliwice, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Tylki-Szyma\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Nutrition and Metabolic Diseases, The Children\u2019s Memorial Health Institute, 04-730 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Lalik","sequence":"additional","affiliation":[{"name":"Department of Systems Biology and Engineering, Silesian University of Technology, 44-100 Gliwice, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/S0959-437X(97)80153-0","article-title":"The sulfatase gene family","volume":"7","author":"Parenti","year":"1997","journal-title":"Curr. Opin. Genet. Dev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1007\/s00018-007-7175-y","article-title":"Human sulfatases: A structural perspective to catalysis","volume":"64","author":"Ghosh","year":"2007","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1038\/sj.ejhg.5200887","article-title":"Molecular and biochemical characterisation of a novel sulphatase gene: Arylsulfatase G (ARSG)","volume":"10","author":"Ferrante","year":"2002","journal-title":"Eur. J. Hum. Genet."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"11388","DOI":"10.1074\/jbc.M709917200","article-title":"Arylsulfatase G, a Novel Lysosomal Sulfatase","volume":"283","author":"Frese","year":"2008","journal-title":"J. Boil. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"27992","DOI":"10.1074\/jbc.M114.584144","article-title":"Molecular characterization of arylsulfatase G expression, processing, glycosylation, transport and activity","volume":"289","author":"Kowalewski","year":"2014","journal-title":"J. Biol. Chem."},{"key":"ref_6","first-page":"482","article-title":"Characterization of the arylsulfatase I (ARSI) gene preferentially expressed in the human retinal pigment epithelium cell line ARPE-19","volume":"15","author":"Oshikawa","year":"2009","journal-title":"Mol. Vis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S0076-6879(05)00016-9","article-title":"Three-Dimensional Structures of Sulfatases","volume":"400","author":"Ghosh","year":"2005","journal-title":"Methods Enzymol."},{"key":"ref_8","first-page":"341","article-title":"The Evolutionary Conservation of a Novel Protein Modification, the Conversion of Cysteine to Serinesemialdehyde in Arylsulfatase from Volvox carteri","volume":"238","author":"Selmer","year":"1996","journal-title":"JBIC J. Boil. Inorg. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6096","DOI":"10.1074\/jbc.273.11.6096","article-title":"Sulfatases, Trapping of the Sulfated Enzyme Intermediate by Substituting the Active Site Formylglycine","volume":"273","author":"Recksiek","year":"1998","journal-title":"J. Boil. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/1750-1172-5-5","article-title":"Mucopolysaccharidosis VI","volume":"5","author":"Vassili","year":"2010","journal-title":"Orphanet J. Rare Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1016\/j.actbio.2013.09.039","article-title":"Molecular engineering of glycosaminoglycan chemistry for biomolecule delivery","volume":"10","author":"Miller","year":"2013","journal-title":"Acta Biomater."},{"key":"ref_12","first-page":"401","article-title":"Vascular myocytes\u2014The multifunctional cells","volume":"9","author":"Hinek","year":"1982","journal-title":"Post\u0119py Biol. Kom\u00f3rki"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"48640","DOI":"10.1074\/jbc.M407241200","article-title":"Dermatan Sulfate Proteoglycan and Glycosaminoglycan Synthesis Is Induced in Fibroblasts by Transfer to a Three-dimensional Extracellular Environment","volume":"279","author":"Lee","year":"2004","journal-title":"J. Boil. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"28116","DOI":"10.1074\/jbc.273.43.28116","article-title":"Dermatan Sulfate Released after Injury Is a Potent Promoter of Fibroblast Growth Factor-2 Function","volume":"273","author":"Penc","year":"1998","journal-title":"J. Boil. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"42815","DOI":"10.1074\/jbc.M204959200","article-title":"Dermatan Sulfate Binds and Potentiates Activity of Keratinocyte Growth Factor (FGF-7)","volume":"277","author":"Trowbridge","year":"2002","journal-title":"J. Boil. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2012\/471325","article-title":"Glycosaminoglycan Storage Disorders: A Review","volume":"2012","author":"Coutinho","year":"2012","journal-title":"Biochem. Res. Int."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1002\/humu.20534","article-title":"Mutational analysis of 105 mucopolysaccharidosis type VI patients","volume":"28","author":"Karageorgos","year":"2007","journal-title":"Hum. Mutat."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"385","DOI":"10.2741\/4490","article-title":"Mucopolysaccharidosis VI: Patophysiology, diagnosis and treatmnt","volume":"22","author":"Harmatz","year":"2017","journal-title":"Front. Biosci.-Landmrk"},{"key":"ref_19","first-page":"11","article-title":"Human pulmonary artery endothelial cells in the model of mucopolysaccharidosis VI present a prohypertensive phenotype","volume":"3","author":"Golda","year":"2015","journal-title":"Mol. Genet. Metab. Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1021\/bm500224f","article-title":"Decorin Mimic Regulates Platelet-Derived Growth Factor and Interferon-\u03b3 Stimulation of Vascular Smooth Muscle Cells","volume":"15","author":"Scott","year":"2014","journal-title":"Biomacromolecules"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s13221-015-0033-z","article-title":"Mechanical stretch: physiological and pathological implications for human vascular endothelial cells","volume":"7","author":"Jufri","year":"2015","journal-title":"Vasc. Cell"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"906","DOI":"10.1016\/j.cca.2011.01.016","article-title":"The dermatan sulfate-dependent anticoagulant pathway is mostly preserved in aneurysm and in severe atherosclerotic lesions while the heparan sulfate pathway is disrupted","volume":"412","author":"Tovar","year":"2011","journal-title":"Clin. Chim. Acta"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1002\/term.2035","article-title":"Decorin mimic promotes endothelial cell health in endothelial monolayers and endothelial-smooth muscle co-cultures","volume":"11","author":"Scott","year":"2015","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Scott, R.A., Paderi, J.E., Sturek, M., and Panitch, A. (2013). Decorin Mimic Inhibits Vascular Smooth Muscle Proliferation and Migration. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0082456"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.carres.2012.03.036","article-title":"Low molecular mass dermatan sulfate modulates endothelial cells proliferation and migration","volume":"356","author":"Rasente","year":"2012","journal-title":"Carbohydr. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1021\/cb500897w","article-title":"Formylglycine, a Post-Translationally Generated Residue with Unique Catalytic Capabilities and Biotechnology Applications","volume":"10","author":"Appel","year":"2014","journal-title":"ACS Chem. Boil."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Smyth, G.K. (2005). limma: Linear Models for Microarray Data. Bioinformatics and Computational Biology Solutions Using R and Bioconductor, Springer Science and Business Media LLC.","DOI":"10.1007\/0-387-29362-0_23"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"R80","DOI":"10.1186\/gb-2004-5-10-r80","article-title":"Bioconductor: open software development for computational biology and bioinformatics","volume":"5","author":"Gentleman","year":"2004","journal-title":"Genome Boil."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1214\/lnms\/1215091155","article-title":"Normalization for two-color cDNA microarray data","volume":"40","author":"Yang","year":"2003","journal-title":"Lect. Notes-Monograph Series"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1093\/bioinformatics\/19.2.185","article-title":"A comparison of normalization methods for high density oligonucleotide array data based on variance and bias","volume":"19","author":"Bolstad","year":"2003","journal-title":"Bioinformatics"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3499","DOI":"10.1021\/acs.jctc.5b00356","article-title":"Improved Peptide and Protein Torsional Energetics with the OPLS-AA Force Field","volume":"11","author":"Robertson","year":"2015","journal-title":"J. Chem. Theory Comput."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/14\/4913\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:50:28Z","timestamp":1760176228000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/14\/4913"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,12]]},"references-count":32,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2020,7]]}},"alternative-id":["ijms21144913"],"URL":"https:\/\/doi.org\/10.3390\/ijms21144913","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,12]]}}}