{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:04:44Z","timestamp":1791414284841,"version":"4.3.3"},"reference-count":69,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T00:00:00Z","timestamp":1594944000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["Project PI14\/00119"],"award-info":[{"award-number":["Project PI14\/00119"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002809","name":"Generalitat de Catalunya","doi-asserted-by":"publisher","award":["Project 2017SGR1796"],"award-info":[{"award-number":["Project 2017SGR1796"]}],"id":[{"id":"10.13039\/501100002809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Seminal plasma proteomics studies could represent a new approach for the determination of molecular elements driving male infertility, resulting in a better male infertility characterization. The aim of this study is to investigate proteomic differences in seminal plasma samples from fertile and infertile individuals. For that, semen samples were selected according to semen analysis, clinical pathology, and values of sperm DNA fragmentation (alkaline and neutral Comet assay and Sperm Chromatin Dispersion test). A total of 24 seminal plasma samples classified in four groups were processed: fertile donors (FD), recurrent miscarriage patients (RM), asthenoteratozoospermic patients (ATZ), and asthenoteratozoospermic patients with varicocele (ATZ-VAR). Results obtained by 2D-differential gel electrophoresis (2D-DIGE) revealed 26 spots significantly increased in fertile donors when compared to patient groups. Also, eight spots in the ATZ group and two in the ATZ-VAR group were decreased compared to the other groups. Twenty-eight proteins were identified by mass spectrometry (MS), most of them involved in metabolic and cellular processes and with a catalytic or binding function. Protein\u2013protein interactions through Search Tool for the Retrieval of Interacting Genes\/Proteins (STRING) tool suggest that a large part of them were associated with each other. Furthermore, most of them were associated with ubiquitin C, indicating that it could play an important regulation role, resulting in a potential male infertility biomarker.<\/jats:p>","DOI":"10.3390\/ijms21145046","type":"journal-article","created":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T10:22:02Z","timestamp":1594981322000},"page":"5046","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Proteomic Analysis in Seminal Plasma of Fertile Donors and Infertile Patients with Sperm DNA Fragmentation"],"prefix":"10.3390","volume":"21","author":[{"given":"Alba","family":"Fernandez-Encinas","sequence":"first","affiliation":[{"name":"Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agust\u00edn","family":"Garc\u00eda-Peir\u00f3","sequence":"additional","affiliation":[{"name":"Centro de Infertilidad Masculina y An\u00e1lisis de Barcelona (CIMAB), 08192 Sant Quirze del Vall\u00e8s, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7887-7871","authenticated-orcid":false,"given":"Javier","family":"del Rey","sequence":"additional","affiliation":[{"name":"Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9101-2044","authenticated-orcid":false,"given":"Jordi","family":"Ribas-Maynou","sequence":"additional","affiliation":[{"name":"Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Abad","sequence":"additional","affiliation":[{"name":"Servei d\u2019Urologia, Corporaci\u00f3 Sanit\u00e0ria Parc Taul\u00ed, Sabadell, Institut Universitari Parc Taul\u00ed\u2014UAB, 08208 Sabadell, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5385-3061","authenticated-orcid":false,"given":"Maria Jos\u00e9","family":"Amengual","sequence":"additional","affiliation":[{"name":"UDIAT, Centre Diagn\u00f2stic, Corporaci\u00f3 Sanit\u00e0ria Parc Taul\u00ed, Sabadell, Institut Universitari Parc Taul\u00ed\u2014UAB, 08208 Sabadell, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Prada","sequence":"additional","affiliation":[{"name":"Servei de Ginecologia, Hospital Universitari M\u00fatua de Terrassa, 08221 Terrassa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaquima","family":"Navarro","sequence":"additional","affiliation":[{"name":"Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6185-2555","authenticated-orcid":false,"given":"Jordi","family":"Benet","sequence":"additional","affiliation":[{"name":"Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/S0015-0282(16)54469-0","article-title":"Fecundity and infertility in the United States: Incidence and trends","volume":"56","author":"Mosher","year":"1991","journal-title":"Fertil. 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