{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T05:30:05Z","timestamp":1776403805516,"version":"3.51.2"},"reference-count":105,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2020,7,19]],"date-time":"2020-07-19T00:00:00Z","timestamp":1595116800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Polish Ministry of Agriculture and Rural Development","award":["HOR hn-801\/11\/14-PO-0114-002"],"award-info":[{"award-number":["HOR hn-801\/11\/14-PO-0114-002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The roles of endogenous brassinosteroids (BRs) in the modulation of reaction to drought and genetic regulation of this process are still obscure. In this study, a multidirectional analysis was performed on semi-dwarf barley (Hordeum vulgare) Near-Isogenic Lines (NILs) and the reference cultivar \u201cBowman\u201d to get insights into various aspects of metabolic reaction to drought. The NILs are defective in BR biosynthesis or signaling and displayed an enhanced tolerance to drought. The BR metabolism perturbations affected the glucose and fructose accumulation under the control and stress conditions. The BR metabolism abnormalities negatively affected the sucrose accumulation as well. However, during drought, the BR-deficient NILs accumulated higher contents of sucrose than the \u201cBowman\u201d cultivar. Under the control conditions, accumulation of transcripts encoding antioxidant enzymes ascorbate peroxidase (HvAPX) and superoxide dismutase (HvSOD) was BR-dependent. However, during drought, the accumulation of HvAPX transcript was BR-dependent, whereas accumulations of transcripts encoding catalase (HvCAT) and HvSOD were not affected by the BR metabolism perturbations. The obtained results reveal a significant role of BRs in regulation of the HvAPX and HvCAT enzymatic activities under control conditions and the HvAPX and HvSOD activities during physiological reactions to drought.<\/jats:p>","DOI":"10.3390\/ijms21145096","type":"journal-article","created":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T06:08:17Z","timestamp":1595225297000},"page":"5096","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Insights into Metabolic Reactions of Semi-Dwarf, Barley Brassinosteroid Mutants to Drought"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9544-6106","authenticated-orcid":false,"given":"Damian","family":"Gruszka","sequence":"first","affiliation":[{"name":"Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1093-8150","authenticated-orcid":false,"given":"Ewa","family":"Pociecha","sequence":"additional","affiliation":[{"name":"Department of Plant Breeding, Physiology and Seed Science, University of Agriculture in Krakow, 30-239 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Jurczyk","sequence":"additional","affiliation":[{"name":"Department of Plant Breeding, Physiology and Seed Science, University of Agriculture in Krakow, 30-239 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8265-6243","authenticated-orcid":false,"given":"Micha\u0142","family":"Dziurka","sequence":"additional","affiliation":[{"name":"The Franciszek Gorski Institute of Plant Physiology, Polish Academy of Sciences, 30-239 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jakub","family":"Oliwa","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, Institute of Basic Sciences, University of Physical Education, 31-571 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6648-101X","authenticated-orcid":false,"given":"Iwona","family":"Sadura","sequence":"additional","affiliation":[{"name":"The Franciszek Gorski Institute of Plant Physiology, Polish Academy of Sciences, 30-239 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6584-3250","authenticated-orcid":false,"given":"Anna","family":"Janeczko","sequence":"additional","affiliation":[{"name":"The Franciszek Gorski Institute of Plant Physiology, Polish Academy of Sciences, 30-239 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1023\/A:1007201411474","article-title":"Plant responses to drought, acclimation, and stress tolerance","volume":"38","author":"Yordanov","year":"2000","journal-title":"Photosynthetica"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1365-3040.2007.01727.x","article-title":"The effect of drought and heat stress on reproductive processes in cereals","volume":"31","author":"Barnabas","year":"2008","journal-title":"Plant Cell Environ."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"86","DOI":"10.3389\/fpls.2014.00086","article-title":"Response of plants to water stress","volume":"5","author":"Osakabe","year":"2014","journal-title":"Front. 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