{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:04:48Z","timestamp":1760238288127,"version":"build-2065373602"},"reference-count":54,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T00:00:00Z","timestamp":1595462400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP-311262"],"award-info":[{"award-number":["MOP-311262"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Pathological and healthy skin models were reconstructed using similar culture conditions according to well-known tissue engineering protocols. For both models, cyclic nucleotide enhancers were used as additives to promote keratinocytes\u2019 proliferation. Cholera toxin (CT) and isoproterenol (ISO), a beta-adrenergic agonist, are the most common cAMP stimulators recommended for cell culture. The aim of this study was to evaluate the impact of either CT or ISO on the pathological characteristics of the dermatosis while producing a psoriatic skin model. Healthy and psoriatic skin substitutes were produced according to the self-assembly method of tissue engineering, using culture media supplemented with either CT (10\u221210 M) or ISO (10\u22126 M). Psoriatic substitutes produced with CT exhibited a more pronounced psoriatic phenotype than those produced with ISO. Indeed, the psoriatic substitutes produced with CT had the thickest epidermis, as well as contained the most proliferating cells and the most altered expression of involucrin, filaggrin, and keratin 10. Of the four conditions under study, psoriatic substitutes produced with CT had the highest levels of cAMP and enhanced expression of adenylate cyclase 9. Taken together, these results suggest that high levels of cAMP are linked to a stronger psoriatic phenotype.<\/jats:p>","DOI":"10.3390\/ijms21155215","type":"journal-article","created":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T10:14:06Z","timestamp":1595499246000},"page":"5215","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A Tissue-Engineered Human Psoriatic Skin Model to Investigate the Implication of cAMP in Psoriasis: Differential Impacts of Cholera Toxin and Isoproterenol on cAMP Levels of the Epidermis"],"prefix":"10.3390","volume":"21","author":[{"given":"M\u00e9lissa","family":"Simard","sequence":"first","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Axe M\u00e9decine R\u00e9g\u00e9n\u00e9ratrice, Centre de Recherche du CHU de Qu\u00e9bec\u2014Universit\u00e9 Laval, Qu\u00e9bec, QC G1J 1Z4, Canada"},{"name":"Facult\u00e9 de Pharmacie, Universit\u00e9 Laval, Qu\u00e9bec, QC G1V 0A6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Morin","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Axe M\u00e9decine R\u00e9g\u00e9n\u00e9ratrice, Centre de Recherche du CHU de Qu\u00e9bec\u2014Universit\u00e9 Laval, Qu\u00e9bec, QC G1J 1Z4, Canada"},{"name":"Facult\u00e9 de Pharmacie, Universit\u00e9 Laval, Qu\u00e9bec, QC G1V 0A6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8456-058X","authenticated-orcid":false,"given":"Genevi\u00e8ve","family":"Rioux","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Axe M\u00e9decine R\u00e9g\u00e9n\u00e9ratrice, Centre de Recherche du CHU de Qu\u00e9bec\u2014Universit\u00e9 Laval, Qu\u00e9bec, QC G1J 1Z4, Canada"},{"name":"Facult\u00e9 de Pharmacie, Universit\u00e9 Laval, Qu\u00e9bec, QC G1V 0A6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachelle","family":"S\u00e9guin","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Axe M\u00e9decine R\u00e9g\u00e9n\u00e9ratrice, Centre de Recherche du CHU de Qu\u00e9bec\u2014Universit\u00e9 Laval, Qu\u00e9bec, QC G1J 1Z4, Canada"},{"name":"Facult\u00e9 de Pharmacie, Universit\u00e9 Laval, Qu\u00e9bec, QC G1V 0A6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Estelle","family":"Loing","sequence":"additional","affiliation":[{"name":"IFF-Lucas Meyer Cosmetics, Qu\u00e9bec, QC G1V 4M6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2423-9599","authenticated-orcid":false,"given":"Roxane","family":"Pouliot","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Axe M\u00e9decine R\u00e9g\u00e9n\u00e9ratrice, Centre de Recherche du CHU de Qu\u00e9bec\u2014Universit\u00e9 Laval, Qu\u00e9bec, QC G1J 1Z4, Canada"},{"name":"Facult\u00e9 de Pharmacie, Universit\u00e9 Laval, Qu\u00e9bec, QC G1V 0A6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/S0140-6736(14)61909-7","article-title":"Psoriasis","volume":"386","author":"Boehncke","year":"2015","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1038\/nature05663","article-title":"Pathogenesis and therapy of psoriasis","volume":"445","author":"Lowes","year":"2007","journal-title":"Nature"},{"key":"ref_3","first-page":"280","article-title":"The impact of psoriasis on quality of life: Results of a 1998 National Psoriasis Foundation patient-membership survey","volume":"137","author":"Krueger","year":"2001","journal-title":"Arch. 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