{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T21:52:18Z","timestamp":1778881938269,"version":"3.51.4"},"reference-count":23,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T00:00:00Z","timestamp":1595462400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The objective of the present study was to analyze the biological and clinical role of the long non-coding RNA LOC642852 in ovarian carcinoma (OC). LOC642852 expression was analyzed in seven OC cell lines (OVCAR-3, OVCAR-8, OVCA 433, OVCA 429, OC 238, DOV13, ES-2) and 139 high-grade serous carcinoma (HGSC) specimens (85 effusions, 54 surgical specimens). Following LOC642852 knockout (KO) using the CRISPR\/Cas9 system, OVCAR-8 HGSC cells were analyzed for spheroid formation, migration, invasion, proliferation, matrix metalloproteinase (MMP) activity, and expression of cell signaling proteins. OVCAR-8 cells with LOC642852 KO were significantly less motile and less invasive compared to controls, with no differences in spheroid formation, proliferation, or matrix metalloproteinase (MMP) activity. Total Akt and Erk levels were comparable in controls and KO cells, but their phosphorylation was significantly increased in the latter. In clinical specimens, LOC642852 was overexpressed in ovarian tumors and omental\/peritoneal metastases compared to effusion specimens (p = 0.013). A non-significant trend for shorter overall (p = 0.109) and progression-free (p = 0.056) survival was observed in patients with HGSC effusions with high LOC642852 levels. Bioinformatics analysis showed potential roles for LOC642852 as part of the TLE3\/miR-221-3p ceRNA network and in relation to the FGFR3 protein. In conclusion, LOC642852 inactivation via CRISPR\/Cas9 affects cell signaling, motility, and invasion in HGSC cells. LOC642852 is differentially expressed in HGSC cells at different anatomical sites. Its potential role in regulating the TLE3\/miR-221-3p ceRNA network and FGFR3 merits further research.<\/jats:p>","DOI":"10.3390\/ijms21155237","type":"journal-article","created":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T11:26:01Z","timestamp":1595503561000},"page":"5237","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The Biological and Clinical Role of the Long Non-Coding RNA LOC642852 in Ovarian Carcinoma"],"prefix":"10.3390","volume":"21","author":[{"given":"Natalie","family":"Filippov-Levy","sequence":"first","affiliation":[{"name":"Institute of Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 91120, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1684-5967","authenticated-orcid":false,"given":"Reuven","family":"Reich","sequence":"additional","affiliation":[{"name":"Institute of Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 91120, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ben","family":"Davidson","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, N-0316 Oslo, Norway"},{"name":"Department of Pathology, Oslo University Hospital, Norwegian Radium Hospital, N-0310 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1093\/nsr\/nwu008","article-title":"Non-coding RNA: A new frontier in regulatory biology","volume":"1","author":"Fu","year":"2014","journal-title":"Natl. Sci Rev."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Rao, M.R.S. (2017). Long Non Coding RNA Biology, Advances in Experimental Medicine and Biology Springer Nature Singapore Pte Ltd.","DOI":"10.1007\/978-981-10-5203-3"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3965","DOI":"10.1158\/0008-5472.CAN-16-2634","article-title":"Long noncoding RNA and cancer: A new paradigm","volume":"77","author":"Bhan","year":"2017","journal-title":"Cancer Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3322\/caac.21551","article-title":"Cancer statistics","volume":"69","author":"Siegel","year":"2019","journal-title":"CA Cancer J. Clin."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3322\/caac.21492","article-title":"Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"68","author":"Bray","year":"2018","journal-title":"CA Cancer J. Clin."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1016\/S0140-6736(18)32552-2","article-title":"Epithelial ovarian cancer","volume":"393","author":"Lheureux","year":"2019","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.cca.2018.12.013","article-title":"LncRNAs in ovarian cancer","volume":"490","author":"Wang","year":"2019","journal-title":"Clin. Chim. Acta."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1186\/s13046-018-0793-4","article-title":"Long non-coding RNAs in ovarian cancer","volume":"37","author":"Zhan","year":"2018","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1016\/j.drudis.2018.04.010","article-title":"Role of lncRNAs in ovarian cancer: Defining new biomarkers for therapeutic purposes","volume":"23","author":"Tripathi","year":"2018","journal-title":"Drug Discov Today"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1111\/j.1582-4934.2010.01148.x","article-title":"miRNA profiling along tumour progression in ovarian carcinoma","volume":"15","author":"Vaksman","year":"2011","journal-title":"J. Cell. Mol. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.ygyno.2018.01.004","article-title":"Expression and clinical role of long non-coding RNA in high-grade serous carcinoma","volume":"148","author":"Smith","year":"2018","journal-title":"Gynecol. Oncol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e11481","DOI":"10.1097\/MD.0000000000011481","article-title":"Altered long noncoding RNAs and survival outcomes in ovarian cancer: A systematic review and meta-analysis (PRISMA Compliant)","volume":"97","author":"Ning","year":"2018","journal-title":"Medicine (Baltimore)"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"16052","DOI":"10.18632\/oncotarget.14109","article-title":"Inhibition of the fibroblast growth factor receptor (FGFR) pathway: The current landscape and barriers to clinical application","volume":"8","author":"Chae","year":"2017","journal-title":"Oncotarget"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1186\/s13046-016-0426-8","article-title":"TLE3 represses colorectal cancer proliferation by inhibiting MAPK and AKT signaling pathways","volume":"35","author":"Yang","year":"2016","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1158\/1055-9965.EPI-11-0917","article-title":"TLE3 expression is associated with sensitivity to taxane treatment in ovarian carcinoma","volume":"21","author":"Samimi","year":"2012","journal-title":"Cancer Epidemio.l Biomarkers Prev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"152515","DOI":"10.1016\/j.prp.2019.152515","article-title":"LINC00205 promotes proliferation, migration and invasion of HCC cells by targeting miR-122-5p","volume":"215","author":"Zhang","year":"2019","journal-title":"Pathol. Res. Pract."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3013","DOI":"10.1002\/jcp.29206","article-title":"LINC00205 modulates the expression of EPHX1 through the inhibition of miR-184 in hepatocellular carcinoma as a ceRNA","volume":"235","author":"Long","year":"2020","journal-title":"J. Cell. Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2932","DOI":"10.1002\/cam4.1180","article-title":"A comprehensive genome-wide analysis of long noncoding RNA expression profile in hepatocellular carcinoma","volume":"6","author":"Cui","year":"2017","journal-title":"Cancer Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5636","DOI":"10.1002\/jcb.27847","article-title":"An eight-long noncoding RNA expression signature for colorectal cancer patients\u2019 prognosis","volume":"120","author":"Zhang","year":"2019","journal-title":"J. Cell. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1093\/carcin\/bgy069","article-title":"LncRNA co-expression network analysis reveals novel biomarkers for pancreatic cancer","volume":"39","author":"Giulietti","year":"2018","journal-title":"Carcinogenesis"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1023\/A:1006550503612","article-title":"Regulation of matrix metalloproteinase-2 (gelatinase A, MMP-2), membrane-type matrix metalloproteinase-1 (MT1-MMP) and tissue inhibitor of metalloproteinases-2 (TIMP-2) expression by elastin-derived peptides in human HT-1080 fibrosarcoma cell line","volume":"16","author":"Brassart","year":"1998","journal-title":"Clin. Exp. Metastasis"},{"key":"ref_22","unstructured":"Kurman, R.J., Carcangiu, M.L., Herrington, C.S., and Young, R.H. (2014). WHO Classification of Tumors of Female Reproductive Organs, International Agency for Research on Cancer. [4th ed.]."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Davidson, B., Firat, P., and Michael, C.W. (2018). Ovarian carcinoma. Serous Effusions\u2014Etiology, Diagnosis, Prognosis and Therapy, Springer. [2nd ed.].","DOI":"10.1007\/978-3-319-76478-8"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/15\/5237\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:51:15Z","timestamp":1760176275000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/15\/5237"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,23]]},"references-count":23,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2020,8]]}},"alternative-id":["ijms21155237"],"URL":"https:\/\/doi.org\/10.3390\/ijms21155237","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,23]]}}}