{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T08:18:13Z","timestamp":1788855493703,"version":"build-2803163510"},"reference-count":46,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2020,7,24]],"date-time":"2020-07-24T00:00:00Z","timestamp":1595548800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005969","name":"University of Bologna","doi-asserted-by":"publisher","award":["Alma Idea senior; RFO-MIUR2017; BIR 2015-Bando Attrezzature"],"award-info":[{"award-number":["Alma Idea senior; RFO-MIUR2017; BIR 2015-Bando Attrezzature"]}],"id":[{"id":"10.13039\/501100005969","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Hydrogen sulfide (H2S) is now considered not only for its toxicity, but also as an endogenously produced gas transmitter with multiple physiological roles, also in maintaining and regulating stem cell physiology. In the present work, we evaluated the effect of a common H2S donor, NaHS, on porcine vascular wall\u2013mesenchymal stem cells (pVW\u2013MSCs). pVW\u2013MSCs were treated for 24 h with increasing doses of NaHS, and the cell viability, cell cycle, and reactive oxygen species (ROS) production were evaluated. Moreover, the long-term effects of NaHS administration on the noteworthy characteristics of pVW\u2013MSCs were analyzed. The MTT test revealed no alteration in cell viability, however, the cell cycle analysis demonstrated that the highest NaHS dose tested (300 \u03bcM) determined a block in S phase, which did not depend on the ROS production. Moreover, NaHS (10 \u03bcM), continuously administered in culture for 21 days, was able to significantly reduce NG2, Nestin and PDGFR-\u03b2 expression. The pro-angiogenic attitude of pVW\u2013MSCs was partially reduced by NaHS: the cells maintained the ability to grow in spheroid and sprouting from that, but endothelial markers (Factor VIII and CD31) were reduced. In conclusion, NaHS can be toxic for pVW\u2013MSCs in high doses, while in low doses, it influences cellular physiology, by affecting the gene expression with a slowing down of the endothelial lineage.<\/jats:p>","DOI":"10.3390\/ijms21155267","type":"journal-article","created":{"date-parts":[[2020,7,24]],"date-time":"2020-07-24T11:16:35Z","timestamp":1595589395000},"page":"5267","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effects of Hydrogen Sulfide Donor NaHS on Porcine Vascular Wall-Mesenchymal Stem Cells"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3384-0244","authenticated-orcid":false,"given":"Chiara","family":"Bernardini","sequence":"first","affiliation":[{"name":"Department of Veterinary Medical Science, University of Bologna, Via Tolara di Sopra, 50-40064 Ozzano Emilia (BO), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5649-1852","authenticated-orcid":false,"given":"Debora","family":"La Mantia","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Science, University of Bologna, Via Tolara di Sopra, 50-40064 Ozzano Emilia (BO), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8569-7158","authenticated-orcid":false,"given":"Salvatore","family":"Nesci","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Science, University of Bologna, Via Tolara di Sopra, 50-40064 Ozzano Emilia (BO), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Salaroli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Science, University of Bologna, Via Tolara di Sopra, 50-40064 Ozzano Emilia (BO), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1742-9378","authenticated-orcid":false,"given":"Cristina","family":"Algieri","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Science, University of Bologna, Via Tolara di Sopra, 50-40064 Ozzano Emilia (BO), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Pagliarani","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Science, University of Bologna, Via Tolara di Sopra, 50-40064 Ozzano Emilia (BO), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2972-4921","authenticated-orcid":false,"given":"Augusta","family":"Zannoni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Science, University of Bologna, Via Tolara di Sopra, 50-40064 Ozzano Emilia (BO), Italy"},{"name":"Health Sciences and Technologies\u2014Interdepartmental Center for Industrial Research (CIRI-SDV), Alma Mater Studiorum\u2014University of Bologna, 40100 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1310-6202","authenticated-orcid":false,"given":"Monica","family":"Forni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Science, University of Bologna, Via Tolara di Sopra, 50-40064 Ozzano Emilia (BO), Italy"},{"name":"Health Sciences and Technologies\u2014Interdepartmental Center for Industrial Research (CIRI-SDV), Alma Mater Studiorum\u2014University of Bologna, 40100 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11788","DOI":"10.1039\/C4CC00968A","article-title":"Hydrogen sulfide (H2S) releasing agents: Chemistry and biological applications","volume":"50","author":"Zhao","year":"2014","journal-title":"Chem. 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