{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T19:26:37Z","timestamp":1790277997390,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T00:00:00Z","timestamp":1595808000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016307","name":"APIS-GENE","doi-asserted-by":"publisher","award":["Embryomim\u00e9tisme"],"award-info":[{"award-number":["Embryomim\u00e9tisme"]}],"id":[{"id":"10.13039\/100016307","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003032","name":"ANRT","doi-asserted-by":"publisher","award":["2017\/0684"],"award-info":[{"award-number":["2017\/0684"]}],"id":[{"id":"10.13039\/501100003032","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CPER","award":["Biom\u00e9dicaments"],"award-info":[{"award-number":["Biom\u00e9dicaments"]}]},{"name":"Union Evolution","award":["no grant number"],"award-info":[{"award-number":["no grant number"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Oviduct fluid extracellular vesicles (oEVs) have been proposed as bringing key molecules to the early developing embryo. In order to evaluate the changes induced by oEVs on embryo phospholipids, fresh bovine blastocysts developed in vitro in the presence or absence of oEVs were analyzed by intact cell MALDI-TOF (Matrix assisted laser desorption ionization\u2014Time of flight) mass spectrometry (ICM-MS). The development rates, cryotolerance, and total cell number of blastocysts were also evaluated. The exposure to oEVs did not affect blastocyst yield or cryotolerance but modified the phospholipid content of blastocysts with specific changes before and after blastocoel expansion. The annotation of differential peaks due to oEV exposure evidenced a shift of embryo phospholipids toward more abundant phosphatidylcholines (PC), phosphatidylethanolamines (PE), and sphingomyelins (SM) with long-chain fatty acids. The lipidomic profiling of oEVs showed that 100% and 33% of the overabundant masses in blastocysts and expanded blastocysts, respectively, were also present in oEVs. In conclusion, this study provides the first analysis of the embryo lipidome regulated by oEVs. Exposure to oEVs induced significant changes in the phospholipid composition of resulting embryos, probably mediated by the incorporation of oEV-phospholipids into embryo membranes and by the modulation of the embryonic lipid metabolism by oEV molecular cargos.<\/jats:p>","DOI":"10.3390\/ijms21155326","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T10:16:49Z","timestamp":1595931409000},"page":"5326","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Oviduct Fluid Extracellular Vesicles Change the Phospholipid Composition of Bovine Embryos Developed In Vitro"],"prefix":"10.3390","volume":"21","author":[{"given":"Charles","family":"Banliat","sequence":"first","affiliation":[{"name":"INRAE, CNRS, University of Tours, IFCE, UMR 85 PRC, F-37380 Nouzilly, France"},{"name":"Union Evolution, F-35530 Noyal-Sur-Vilaine, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Le Bourhis","sequence":"additional","affiliation":[{"name":"Allice, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oph\u00e9lie","family":"Bernardi","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, University of Tours, IFCE, UMR 85 PRC, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Tomas","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, University of Tours, IFCE, UMR 85 PRC, F-37380 Nouzilly, France"},{"name":"INRAE, Universit\u00e9 de Tours, CHU de Tours, Plate-forme CIRE, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Labas","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, University of Tours, IFCE, UMR 85 PRC, F-37380 Nouzilly, France"},{"name":"INRAE, Universit\u00e9 de Tours, CHU de Tours, Plate-forme CIRE, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4534-9949","authenticated-orcid":false,"given":"Pascal","family":"Salvetti","sequence":"additional","affiliation":[{"name":"Allice, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8257-4224","authenticated-orcid":false,"given":"Beno\u00eet","family":"Guyonnet","sequence":"additional","affiliation":[{"name":"Union Evolution, F-35530 Noyal-Sur-Vilaine, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9836-2506","authenticated-orcid":false,"given":"Pascal","family":"Mermillod","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, University of Tours, IFCE, UMR 85 PRC, F-37380 Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4569-5665","authenticated-orcid":false,"given":"Marie","family":"Saint-Dizier","sequence":"additional","affiliation":[{"name":"INRAE, CNRS, University of Tours, IFCE, UMR 85 PRC, F-37380 Nouzilly, France"},{"name":"Department Agrosciences, Faculty of Sciences and Techniques, University of Tours, F-37200 Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1530\/REP-12-0279","article-title":"Roles of the oviduct in mammalian fertilization","volume":"144","author":"Coy","year":"2012","journal-title":"Reproduction"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1111\/j.1469-185X.2011.00196.x","article-title":"Components of oviduct physiology in eutherian mammals","volume":"287","author":"Hunter","year":"2012","journal-title":"Biol Rev. 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