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This study aimed to reveal the influence of spermatozoa, seminal plasma, or natural mating on mRNA expression of RBPs and TRP ion channels in different segments of the internal genital tract of oestrous, preovulatory sows. Particularly, we focused on mRNA expression changes of the cold-inducible proteins (CIPs) and related TRP channels. Pre-ovulatory sows were naturally mated (NM) or cervically infused with semen (Semen-AI) or sperm-free seminal plasma either from the entire ejaculate (SP-TOTAL) or the sperm-rich fraction (SP-AI). Samples (cervix to infundibulum) were collected by laparotomy under general anaesthesia for transcriptomic analysis (GeneChip\u00ae Porcine Gene 1.0 ST Array) 24 h after treatments. The NM treatment induced most of the mRNA expression changes, compared to Semen-AI, SP-AI, and SP-TOTAL treatments including unique significative changes in CIRBP, RBM11, RBM15B, RBMS1, TRPC1, TRPC4, TRPC7, and TRPM8. The findings on the differential mRNA expression on RBPs and TRP ion channels, especially to CIPs and related TRP ion channels, suggest that spermatozoa and seminal plasma differentially modulated both protein families during the preovulatory phase, probably related to a still unknown early signalling mechanism in the sow reproductive tract.<\/jats:p>","DOI":"10.3390\/ijms21155333","type":"journal-article","created":{"date-parts":[[2020,7,28]],"date-time":"2020-07-28T10:16:49Z","timestamp":1595931409000},"page":"5333","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The Expression of Cold-Inducible RNA-Binding Protein mRNA in Sow Genital Tract Is Modulated by Natural Mating, But Not by Seminal Plasma"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7524-2088","authenticated-orcid":false,"given":"Jaume","family":"Gardela","sequence":"first","affiliation":[{"name":"Department of Biomedical and Clinical Sciences (BKV), Division of Children\u2019s and Women Health (BKH), Obstetrics and Gynaecology, Link\u00f6ping University, 58185 Link\u00f6ping, Sweden"},{"name":"Department of Animal Health and Anatomy, Veterinary Faculty, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7092-7993","authenticated-orcid":false,"given":"Mateo","family":"Ruiz-Conca","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Clinical Sciences (BKV), Division of Children\u2019s and Women Health (BKH), Obstetrics and Gynaecology, Link\u00f6ping University, 58185 Link\u00f6ping, Sweden"},{"name":"Department of Animal Health and Anatomy, Veterinary Faculty, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6811-0191","authenticated-orcid":false,"given":"Cristina A.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Clinical Sciences (BKV), Division of Children\u2019s and Women Health (BKH), Obstetrics and Gynaecology, Link\u00f6ping University, 58185 Link\u00f6ping, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic","family":"Wright","sequence":"additional","affiliation":[{"name":"Department of Physics, Chemistry and Biology, Faculty of Science and Engineering, Link\u00f6ping University, 58185 Link\u00f6ping, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manel","family":"L\u00f3pez-B\u00e9jar","sequence":"additional","affiliation":[{"name":"Department of Animal Health and Anatomy, Veterinary Faculty, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"},{"name":"College of Veterinary Medicine, Western University of Health Sciences, Pomona, CA 91766, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5194-2124","authenticated-orcid":false,"given":"Heriberto","family":"Rodriguez-Martinez","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Clinical Sciences (BKV), Division of Children\u2019s and Women Health (BKH), Obstetrics and Gynaecology, Link\u00f6ping University, 58185 Link\u00f6ping, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0120-354X","authenticated-orcid":false,"given":"Manuel","family":"Alvarez-Rodriguez","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Clinical Sciences (BKV), Division of Children\u2019s and Women Health (BKH), Obstetrics and Gynaecology, Link\u00f6ping University, 58185 Link\u00f6ping, Sweden"},{"name":"Department of Animal Health and Anatomy, Veterinary Faculty, Universitat Aut\u00f2noma de Barcelona, 08193 Bellaterra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.theriogenology.2004.09.028","article-title":"Boar spermatozoa in the oviduct","volume":"63","author":"Saravia","year":"2005","journal-title":"Theriogenology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1002\/mrd.22674","article-title":"The role of mating in oviduct biology","volume":"83","author":"Reuquen","year":"2016","journal-title":"Mol. 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