{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T11:09:54Z","timestamp":1782299394953,"version":"3.54.5"},"reference-count":33,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T00:00:00Z","timestamp":1596412800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/S004505\/1"],"award-info":[{"award-number":["EP\/S004505\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"name":"N8 Agrifood","award":["Food2Bone"],"award-info":[{"award-number":["Food2Bone"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>To improve the integration of a biomaterial with surrounding tissue, its surface properties may be modified by adsorption of biomacromolecules, e.g., fibrils. Whey protein isolate (WPI), a dairy industry by-product, supports osteoblastic cell growth. WPI\u2019s main component, \u03b2-lactoglobulin, forms fibrils in acidic solutions. In this study, aiming to develop coatings for biomaterials for bone contact, substrates were coated with WPI fibrils obtained at pH 2 or 3.5. Importantly, WPI fibrils coatings withstood autoclave sterilization and appeared to promote spreading and differentiation of human bone marrow stromal cells (hBMSC). In the future, WPI fibrils coatings could facilitate immobilization of biomolecules with growth stimulating or antimicrobial properties.<\/jats:p>","DOI":"10.3390\/ijms21155544","type":"journal-article","created":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T06:16:47Z","timestamp":1596435407000},"page":"5544","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Dairy-Inspired Coatings for Bone Implants from Whey Protein Isolate-Derived Self-Assembled Fibrils"],"prefix":"10.3390","volume":"21","author":[{"given":"Rebecca","family":"Rabe","sequence":"first","affiliation":[{"name":"Division of Food Technology, Kiel University, 24118 Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Hempel","sequence":"additional","affiliation":[{"name":"Institute of Physiological Chemistry, Technische Universit\u00e4t Dresden, 01069 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7579-965X","authenticated-orcid":false,"given":"Laurine","family":"Martocq","sequence":"additional","affiliation":[{"name":"Engineering Department, Lancaster University, Lancaster LA1 4YW, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4183-1794","authenticated-orcid":false,"given":"Julia K.","family":"Keppler","sequence":"additional","affiliation":[{"name":"Division of Food Technology, Kiel University, 24118 Kiel, Germany"},{"name":"Laboratory of Food Process Engineering, Wageningen University &amp; Research AFSG, 6708 PB Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6891-5133","authenticated-orcid":false,"given":"Jenny","family":"Aveyard","sequence":"additional","affiliation":[{"name":"School of Engineering, University of Liverpool, Liverpool L69 3BX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy E. L.","family":"Douglas","sequence":"additional","affiliation":[{"name":"Engineering Department, Lancaster University, Lancaster LA1 4YW, UK"},{"name":"Materials Science Institute (MSI), Lancaster University, Lancaster LA1 4YW, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"28","DOI":"10.3168\/jds.2017-13119","article-title":"Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells","volume":"101","author":"Douglas","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Gupta, D., Kocot, M., Tryba, A.M., Serafim, A., Stancu, I.C., Jaegermann, Z., Pamu\u0142a, E., Reilly, G.C., and Douglas, T.E.L. (2020). Novel naturally derived whey protein isolate and aragonite biocomposite hydrogels have potential for bone regeneration. 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