{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T09:47:28Z","timestamp":1776246448154,"version":"3.50.1"},"reference-count":55,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T00:00:00Z","timestamp":1598400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Apicomplexan parasites, through their motor machinery, produce the required propulsive force critical for host cell-entry. The conserved components of this so-called glideosome machinery are myosin A and myosin A Tail Interacting Protein (MTIP). MTIP tethers myosin A to the inner membrane complex of the parasite through 20 amino acid-long C-terminal end of myosin A that makes direct contacts with MTIP, allowing the invasion of Plasmodium falciparum in erythrocytes. Here, we discovered through screening a peptide library, a de-novo peptide ZA1 that binds the myosin A tail domain. We demonstrated that ZA1 bound strongly to myosin A tail and was able to disrupt the native myosin A tail MTIP complex both in vitro and in vivo. We then showed that a shortened peptide derived from ZA1, named ZA1S, was able to bind myosin A and block parasite invasion. Overall, our study identified a novel anti-malarial peptide that could be used in combination with other antimalarials for blocking the invasion of Plasmodium falciparum.<\/jats:p>","DOI":"10.3390\/ijms21176158","type":"journal-article","created":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T09:05:37Z","timestamp":1598432737000},"page":"6158","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A De novo Peptide from a High Throughput Peptide Library Blocks Myosin A -MTIP Complex Formation in Plasmodium falciparum"],"prefix":"10.3390","volume":"21","author":[{"given":"Zill e","family":"Anam","sequence":"first","affiliation":[{"name":"Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7312-6112","authenticated-orcid":false,"given":"Nishant","family":"Joshi","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, School of Natural Sciences, Shiv Nadar University, Greater Noida, Uttar Pradesh 201304, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakshi","family":"Gupta","sequence":"additional","affiliation":[{"name":"School of Physical Sciences, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Preeti","family":"Yadav","sequence":"additional","affiliation":[{"name":"Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayushi","family":"Chaurasiya","sequence":"additional","affiliation":[{"name":"Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amandeep Kaur","family":"Kahlon","sequence":"additional","affiliation":[{"name":"Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shikha","family":"Kaushik","sequence":"additional","affiliation":[{"name":"Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manoj","family":"Munde","sequence":"additional","affiliation":[{"name":"School of Physical Sciences, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anand","family":"Ranganathan","sequence":"additional","affiliation":[{"name":"Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shailja","family":"Singh","sequence":"additional","affiliation":[{"name":"Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,26]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2018). 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