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Depending on the tissue of origin, MSCs have different characteristics and physiological effects. In various cancer studies, MSCs have been found to have either tumor-promoting or tumor-inhibiting action. This study investigated the effect of adipose tissue-MSCs (AT-MSCs) and bone marrow-MSCs (BM-MSCs) on global long interspersed nuclear element-1 (LINE-1) methylation, the expression level of microenvironment remodeling genes and cell proliferation, migration and invasion of oral tongue squamous cell carcinoma (OTSCC). Additionally, we studied the effect of human tongue squamous carcinoma (HSC-3)-conditioned media on LINE-1 methylation and the expression of microenvironment remodeling genes in AT-MSCs and BM-MSCs. Conditioned media from HSC-3 or MSCs did not affect LINE-1 methylation level in either cancer cells or MSCs, respectively. In HSC-3 cells, no effect of MSCs-conditioned media was detected on the expression of ICAM1, ITGA3 or MMP1. On the other hand, HSC-3-conditioned media upregulated ICAM1 and MMP1 expression in both types of MSCs. Co-cultures of AT-MSCs with HSC-3 did not induce proliferation, migration or invasion of the cancer cells. In conclusion, AT-MSCs, unlike BM-MSCs, seem not to participate in oral cancer progression.<\/jats:p>","DOI":"10.3390\/ijms21186455","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T11:24:24Z","timestamp":1599218664000},"page":"6455","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8080-5305","authenticated-orcid":false,"given":"Snehadri","family":"Sinha","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki, 00014 Helsinki, Finland"},{"name":"Helsinki University Central Hospital, 00290 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matilda","family":"Narjus-Sterba","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki, 00014 Helsinki, Finland"},{"name":"Translational Immunology Research Program (TRIMM), Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3330-1670","authenticated-orcid":false,"given":"Katja","family":"Tuomainen","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki, 00014 Helsinki, Finland"},{"name":"Translational Immunology Research Program (TRIMM), Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sippy","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki, 00014 Helsinki, Finland"},{"name":"Helsinki University Central Hospital, 00290 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riitta","family":"Sepp\u00e4nen-Kaijansinkko","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki, 00014 Helsinki, Finland"},{"name":"Helsinki University Central Hospital, 00290 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuula","family":"Salo","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki, 00014 Helsinki, Finland"},{"name":"Helsinki University Central Hospital, 00290 Helsinki, Finland"},{"name":"Translational Immunology Research Program (TRIMM), Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland"},{"name":"Cancer and Translational Medicine Research Unit, University of Oulu, 90014 Oulu, Finland"},{"name":"Medical Research Centre, Oulu University Hospital, 90014 Oulu, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina","family":"Mannerstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki, 00014 Helsinki, Finland"},{"name":"Helsinki University Central Hospital, 00290 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1938-2136","authenticated-orcid":false,"given":"Ahmed","family":"Al-Samadi","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Clinicum, University of Helsinki, 00014 Helsinki, Finland"},{"name":"Translational Immunology Research Program (TRIMM), Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2724","DOI":"10.1089\/scd.2011.0722","article-title":"Same or not the same? 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