{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T15:10:28Z","timestamp":1790435428214,"version":"4.1.0"},"reference-count":50,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2020,9,13]],"date-time":"2020-09-13T00:00:00Z","timestamp":1599955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Amphibian skin is a promising natural resource for antimicrobial peptides (AMPs), key effectors of innate immunity with attractive therapeutic potential to fight antibiotic-resistant pathogens. Our previous studies showed that the skin of the Sahara Frog (Pelophylax saharicus) contains broad-spectrum AMPs of the temporin family, named temporins-SH. Here, we focused our study on temporin-SHe, a temporin-SHd paralog that we have previously identified in this frog but was never structurally and functionally characterized. We synthesized and determined the structure of temporin-SHe. This non-amphipathic \u03b1-helical peptide was demonstrated to strongly destabilize the lipid chain packing of anionic multilamellar vesicles mimicking bacterial membranes. Investigation of the antimicrobial activity revealed that temporin-SHe targets Gram-negative and Gram-positive bacteria, including clinical isolates of multi-resistant Staphylococcus aureus strains. Temporin-SHe exhibited also antiparasitic activity toward different Leishmania species responsible for visceral leishmaniasis, as well as cutaneous and mucocutaneous forms. Functional assays revealed that temporin-SHe exerts bactericidal effects with membrane depolarization and permeabilization, via a membranolytic mechanism observed by scanning electron microscopy. Temporin-SHe represents a new member of the very limited group of antiparasitic temporins\/AMPs. Despite its cytotoxicity, it is nevertheless an interesting tool to study the AMP antiparasitic mechanism and design new antibacterial\/antiparasitic agents.<\/jats:p>","DOI":"10.3390\/ijms21186713","type":"journal-article","created":{"date-parts":[[2020,9,13]],"date-time":"2020-09-13T21:11:32Z","timestamp":1600031492000},"page":"6713","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Functional Characterization of Temporin-SHe, a New Broad-Spectrum Antibacterial and Leishmanicidal Temporin-SH Paralog from the Sahara Frog (Pelophylax saharicus)"],"prefix":"10.3390","volume":"21","author":[{"given":"Sonia","family":"Andr\u00e9","sequence":"first","affiliation":[{"name":"CNRS, Institut de Biologie Paris-Seine, IBPS, BIOSIPE, Sorbonne Universit\u00e9, F-75252 Paris, France"},{"name":"Cell death in host-pathogen interactions, CNRS-ERL3649, Universit\u00e9 de Paris, 75006 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahid","family":"Raja","sequence":"additional","affiliation":[{"name":"CNRS, Institut de Biologie Paris-Seine, IBPS, BIOSIPE, Sorbonne Universit\u00e9, F-75252 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6266-3956","authenticated-orcid":false,"given":"Vincent","family":"Humblot","sequence":"additional","affiliation":[{"name":"FEMTO-ST Institute, UMR CNRS 6174, Universit\u00e9 Bourgogne Franche-Comt\u00e9, 25030 Besan\u00e7on CEDEX, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Piesse","sequence":"additional","affiliation":[{"name":"CNRS, Institut de Biologie Paris-Seine, IBPS, Peptide Synthesis Facility, Sorbonne Universit\u00e9, F-75252 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Foulon","sequence":"additional","affiliation":[{"name":"CNRS, Institut de Biologie Paris-Seine, IBPS, BIOSIPE, Sorbonne Universit\u00e9, F-75252 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0034-9291","authenticated-orcid":false,"given":"Denis","family":"Sereno","sequence":"additional","affiliation":[{"name":"IRD, Universit\u00e9 de Montpellier, MiVegec, 34394 Montpellier CEDEX 5, France"},{"name":"IRD, Universit\u00e9 de Montpellier, InterTryp, 34394 Montpellier CEDEX 5, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Oury","sequence":"additional","affiliation":[{"name":"IRD, Universit\u00e9 de Montpellier, InterTryp, 34394 Montpellier CEDEX 5, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8582-1278","authenticated-orcid":false,"given":"Ali","family":"Ladram","sequence":"additional","affiliation":[{"name":"CNRS, Institut de Biologie Paris-Seine, IBPS, BIOSIPE, Sorbonne Universit\u00e9, F-75252 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e216","DOI":"10.1016\/S1473-3099(20)30327-3","article-title":"The value of antimicrobial peptides in the age of resistance","volume":"20","author":"Magana","year":"2020","journal-title":"Lancet Infect. 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PLoS ONE, 8.","DOI":"10.1371\/annotation\/dbb3e614-dc4c-40dd-b9e0-37787ae6b150"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/18\/6713\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:09:38Z","timestamp":1760177378000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/18\/6713"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,13]]},"references-count":50,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2020,9]]}},"alternative-id":["ijms21186713"],"URL":"https:\/\/doi.org\/10.3390\/ijms21186713","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,13]]}}}