{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T06:15:41Z","timestamp":1780640141971,"version":"3.54.1"},"reference-count":26,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T00:00:00Z","timestamp":1600646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2016R1D1A1A02937214 and 2020R1A2C100407711"],"award-info":[{"award-number":["2016R1D1A1A02937214 and 2020R1A2C100407711"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003705","name":"Korea Institute of Toxicology","doi-asserted-by":"publisher","award":["KK-1806-01"],"award-info":[{"award-number":["KK-1806-01"]}],"id":[{"id":"10.13039\/501100003705","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The increase in drug-resistant Mycobacterium abscessus, which has become resistant to existing standard-of-care agents, is a major concern, and new antibacterial agents are strongly needed. In this study, we introduced etamycin that showed an excellent activity against M. abscessus. We found that etamycin significantly inhibited the growth of M. abscessus wild-type strain, three subspecies, and clinical isolates in vitro and inhibited the growth of M. abscessus that resides in macrophages without cytotoxicity. Furthermore, the in vivo efficacy of etamycin in the zebrafish (Danio rerio) infection model was greater than that of clarithromycin, which is recommended as the core agent for treating M. abscessus infections. Thus, we concluded that etamycin is a potential anti-M. abscessus candidate for further development as a clinical drug candidate.<\/jats:p>","DOI":"10.3390\/ijms21186908","type":"journal-article","created":{"date-parts":[[2020,9,21]],"date-time":"2020-09-21T08:18:01Z","timestamp":1600676281000},"page":"6908","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Etamycin as a Novel Mycobacterium abscessus Inhibitor"],"prefix":"10.3390","volume":"21","author":[{"given":"Bui Thi Bich","family":"Hanh","sequence":"first","affiliation":[{"name":"Molecular Mechanisms of Antibiotics, Division of Life Science, Research Institute of Life Science, Division of Applied Life Science (BK21plus Program), Gyeongsang National University, Jinju 52828, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae Ho","family":"Kim","sequence":"additional","affiliation":[{"name":"Molecular Mechanisms of Antibiotics, Division of Life Science, Research Institute of Life Science, Division of Applied Life Science (BK21plus Program), Gyeongsang National University, Jinju 52828, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"June-Woo","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jinju 52843, Korea"},{"name":"Human and Environmental Toxicology Program, Korea University of Science and Technology (UST), Daejeon 34113, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3787-6455","authenticated-orcid":false,"given":"Da-Gyum","family":"Lee","sequence":"additional","affiliation":[{"name":"Molecular Mechanisms of Antibiotics, Division of Life Science, Research Institute of Life Science, Division of Applied Life Science (BK21plus Program), Gyeongsang National University, Jinju 52828, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Sung","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute of Natural Science &amp; Technology, Hanyang University, Ansan 15588, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Eun","family":"Du","sequence":"additional","affiliation":[{"name":"Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4918-961X","authenticated-orcid":false,"given":"Chul-Su","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Molecular &amp; Life Science, Hanyang University, Ansan 15588, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Chan","family":"Oh","sequence":"additional","affiliation":[{"name":"Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jichan","family":"Jang","sequence":"additional","affiliation":[{"name":"Molecular Mechanisms of Antibiotics, Division of Life Science, Research Institute of Life Science, Division of Applied Life Science (BK21plus Program), Gyeongsang National University, Jinju 52828, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1002\/open.202000042","article-title":"Structure-Based Design and Synthesis of Piperidinol-Containing Molecules as New Mycobacterium abscessus Inhibitors","volume":"9","author":"Dupont","year":"2020","journal-title":"ChemistryOpen"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1038\/s41579-020-0331-1","article-title":"Non-tuberculous mycobacteria and the rise of Mycobacterium abscessus","volume":"18","author":"Johansen","year":"2020","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"27","DOI":"10.3389\/fmed.2017.00027","article-title":"Infection sources of a common non-tuberculous mycobacterial pathogen, Mycobacterium avium complex","volume":"4","author":"Nishiuchi","year":"2017","journal-title":"Front. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3690","DOI":"10.1038\/s41598-020-60452-1","article-title":"A mouse model of pulmonary Mycobacteroides abscessus infection","volume":"10","author":"Maggioncalda","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.3389\/fmicb.2019.01977","article-title":"Clinical efficacy and adverse effects of antibiotics used to treat mycobacterium abscessus pulmonary disease","volume":"10","author":"Chen","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.3389\/fmicb.2018.02179","article-title":"The role of antibiotic-target-modifying and antibiotic-modifying enzymes in mycobacterium abscessusdrug resistance","volume":"9","author":"Luthra","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.3389\/fmicb.2018.02417","article-title":"Novel screen to assess bactericidal activity of compounds against non-replicating mycobacterium abscessus","volume":"9","author":"Berube","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_8","first-page":"00154","article-title":"Amikacin exposure and susceptibility of macrolide-resistant Mycobacterium abscessus","volume":"5","author":"Shallom","year":"2019","journal-title":"ERJ Open Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1638","DOI":"10.3201\/2109.141634","article-title":"Mycobacterium abscessus Complex Infections in Humans","volume":"21","author":"Lee","year":"2015","journal-title":"Emerg. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1093\/jac\/dkr578","article-title":"Mycobacterium abscessus: A new antibiotic nightmare","volume":"67","author":"Nessar","year":"2012","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Kim, T.H., Bich Hanh, B.T., Kim, G., Lee, D.G., Park, J.W., Lee, S.E., Kim, J.S., Kim, B.S., Ryoo, S., and Jo, E.K. (2019). Thiostrepton: A Novel Therapeutic Drug Candidate for Mycobacterium abscessus Infection. Molecules, 24.","DOI":"10.3390\/molecules24244511"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1128\/IAI.00835-06","article-title":"Hypervirulence of a rough variant of the Mycobacterium abscessus type strain","volume":"75","author":"Catherinot","year":"2007","journal-title":"Infect. Immun."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1097\/QCO.0000000000000104","article-title":"Mycobacterium abscessus: Challenges in diagnosis and treatment","volume":"27","author":"Benwill","year":"2014","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Koh, W.-J. (2017). Nontuberculous Mycobacteria\u2014Overview. Microbiol. Spectr.","DOI":"10.1128\/microbiolspec.TNMI7-0024-2016"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1111\/mmi.13406","article-title":"A new piperidinol derivative targeting mycolic acid transport in Mycobacterium abscessus","volume":"101","author":"Dupont","year":"2016","journal-title":"Mol. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1502","DOI":"10.1016\/j.drudis.2018.04.001","article-title":"NTM drug discovery: Status, gaps and the way forward","volume":"23","author":"Wu","year":"2018","journal-title":"Drug Discov. Today"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Degiacomi, G., Sammartino, J.C., Chiarelli, L.R., Riabova, O., Makarov, V., and Pasca, M.R. (2019). Mycobacterium abscessus, an emerging and worrisome pathogen among cystic fibrosis patients. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20235868"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2204","DOI":"10.3389\/fmicb.2017.02204","article-title":"Reporter-based assays for high-throughput drug screening against Mycobacterium abscessus","volume":"8","author":"Gupta","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Jeong, J., Kim, G., Moon, C., Kim, H.J., Kim, T.H., and Jang, J. (2018). Pathogen Box screening for hit identification against Mycobacterium abscessus. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0195595"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1093\/jac\/dkr154","article-title":"Identification of antimicrobial activity among FDA-approved drugs for combating Mycobacterium abscessus and Mycobacterium chelonae","volume":"66","author":"Chopra","year":"2011","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e01008","DOI":"10.1128\/AAC.01008-19","article-title":"Extremely low hit rate in a diverse chemical drug screen targeting mycobacterium abscessus","volume":"63","author":"Malin","year":"2019","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Hanh, B.T.B., Park, J.W., Kim, T.H., Kim, J.S., Yang, C.S., Jang, K., Cui, J., Oh, D.C., and Jang, J. (2020). Rifamycin O, An Alternative Anti-Mycobacterium abscessus Agent. Molecules, 25.","DOI":"10.3390\/molecules25071597"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1038\/ja.2010.22","article-title":"Activity of the streptogramin antibiotic etamycin against methicillin-resistant Staphylococcus aureus","volume":"63","author":"Haste","year":"2010","journal-title":"J. Antibiot. (Tokyo)"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Hosoda, K., Koyama, N., Kanamoto, A., and Tomoda, H. (2019). Discovery of Nosiheptide, Griseoviridin, and Etamycin as Potent Anti-Mycobacterial Agents against Mycobacterium avium Complex. Molecules, 24.","DOI":"10.3390\/molecules24081495"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1021\/acs.jnatprod.6b00235","article-title":"Antibacterial Compounds from Marine Bacteria, 2010\u20132015","volume":"80","author":"Schinke","year":"2017","journal-title":"J. Nat. Prod."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1038\/nprot.2013.068","article-title":"Evaluation of the pathogenesis and treatment of Mycobacterium marinum infection in zebrafish","volume":"8","author":"Takaki","year":"2013","journal-title":"Nat. Protoc."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/18\/6908\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:11:53Z","timestamp":1760177513000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/18\/6908"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,21]]},"references-count":26,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2020,9]]}},"alternative-id":["ijms21186908"],"URL":"https:\/\/doi.org\/10.3390\/ijms21186908","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,21]]}}}