{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:02:46Z","timestamp":1790337766357,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T00:00:00Z","timestamp":1601942400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Spallation Source ERIC","award":["projects related to COVID-19"],"award-info":[{"award-number":["projects related to COVID-19"]}]},{"DOI":"10.13039\/501100003252","name":"Lund University","doi-asserted-by":"publisher","award":["Lund Protein Production Platform (LP3)"],"award-info":[{"award-number":["Lund Protein Production Platform (LP3)"]}],"id":[{"id":"10.13039\/501100003252","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), causing Coronavirus Disease 19 (COVID-19), emerged at the end of 2019 and quickly spread to cause a global pandemic with severe socio-economic consequences. The early sequencing of its RNA genome revealed its high similarity to SARS, likely to have originated from bats. The SARS-CoV-2 non-structural protein 10 (nsp10) displays high sequence similarity with its SARS homologue, which binds to and stimulates the 3\u2032-to-5\u2032 exoribonuclease and the 2\u2032-O-methlytransferase activities of nsps 14 and 16, respectively. Here, we report the biophysical characterization and 1.6 \u00c5 resolution structure of the unbound form of nsp10 from SARS-CoV-2 and compare it to the structures of its SARS homologue and the complex-bound form with nsp16 from SARS-CoV-2. The crystal structure and solution behaviour of nsp10 will not only form the basis for understanding the role of SARS-CoV-2 nsp10 as a central player of the viral RNA capping apparatus, but will also serve as a basis for the development of inhibitors of nsp10, interfering with crucial functions of the replication\u2013transcription complex and virus replication.<\/jats:p>","DOI":"10.3390\/ijms21197375","type":"journal-article","created":{"date-parts":[[2020,10,6]],"date-time":"2020-10-06T10:46:17Z","timestamp":1601981177000},"page":"7375","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Crystal Structure of Non-Structural Protein 10 from Severe Acute Respiratory Syndrome Coronavirus-2"],"prefix":"10.3390","volume":"21","author":[{"given":"Annika","family":"Rogstam","sequence":"first","affiliation":[{"name":"Department of Biology &amp; Lund Protein Production Platform, Lund University, S\u00f6lvegatan 35, 22362 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Nyblom","sequence":"additional","affiliation":[{"name":"Department of Biology &amp; Lund Protein Production Platform, Lund University, S\u00f6lvegatan 35, 22362 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Signe","family":"Christensen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Structural Biology, Lund University, Naturvetarv\u00e4gen 26, 22241 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celeste","family":"Sele","sequence":"additional","affiliation":[{"name":"Department of Biology &amp; Lund Protein Production Platform, Lund University, S\u00f6lvegatan 35, 22362 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1135-2744","authenticated-orcid":false,"given":"Vladimir O.","family":"Talibov","sequence":"additional","affiliation":[{"name":"BioMax, MAX IV Laboratory, Fotongatan 2, 22484 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Therese","family":"Lindvall","sequence":"additional","affiliation":[{"name":"Department of Biology &amp; Lund Protein Production Platform, Lund University, S\u00f6lvegatan 35, 22362 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Andersson","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Department of Biology &amp; Lund Protein Production Platform, Lund University, S\u00f6lvegatan 35, 22362 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingemar","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Structural Biology, Lund University, Naturvetarv\u00e4gen 26, 22241 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8287-0269","authenticated-orcid":false,"given":"Zo\u00eb","family":"Fisher","sequence":"additional","affiliation":[{"name":"Department of Biology &amp; Lund Protein Production Platform, Lund University, S\u00f6lvegatan 35, 22362 Lund, Sweden"},{"name":"Scientific Activities Division, Science Directorate, European Spallation Source ERIC, Box 176, SE-221 00 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Knecht","sequence":"additional","affiliation":[{"name":"Department of Biology &amp; Lund Protein Production Platform, Lund University, S\u00f6lvegatan 35, 22362 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6096-9102","authenticated-orcid":false,"given":"Frank","family":"Kozielski","sequence":"additional","affiliation":[{"name":"School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,6]]},"reference":[{"key":"ref_1","first-page":"S9","article-title":"SARS: Epidemiology","volume":"8","author":"Xu","year":"2020","journal-title":"Respirology"},{"key":"ref_2","unstructured":"(2020, June 14). 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