{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:10:43Z","timestamp":1790838643240,"version":"4.1.0"},"reference-count":63,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T00:00:00Z","timestamp":1602201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Reims Champagne-Ardenne","award":["NA"],"award-info":[{"award-number":["NA"]}]},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Research Institute in Public Health","award":["NA"],"award-info":[{"award-number":["NA"]}]},{"name":"National Institute of Cancer","award":["NA"],"award-info":[{"award-number":["NA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Nicotinic acetylcholine receptors (nAChRs) are pentameric ligand-gated ion channels responsible for rapid neural and neuromuscular signal transmission. Although it is well documented that 16 subunits are encoded by the human genome, their presence in airway epithelial cells (AECs) remains poorly understood, and contribution to pathology is mainly discussed in the context of cancer. We analysed nAChR subunit expression in the human lungs of smokers and non-smokers using transcriptomic data for whole-lung tissues, isolated large AECs, and isolated small AECs. We identified differential expressions of nAChRs in terms of detection and repartition in the three modalities. Smoking-associated alterations were also unveiled. Then, we identified an nAChR transcriptomic print at the single-cell level. Finally, we reported the localizations of detectable nAChRs in bronchi and large bronchioles. Thus, we compiled the first complete atlas of pulmonary nAChR subunits to open new avenues to further unravel the involvement of these receptors in lung homeostasis and respiratory diseases.<\/jats:p>","DOI":"10.3390\/ijms21207446","type":"journal-article","created":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T10:19:23Z","timestamp":1602238763000},"page":"7446","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Nicotinic Receptor Subunits Atlas in the Adult Human Lung"],"prefix":"10.3390","volume":"21","author":[{"given":"Zania","family":"Diabasana","sequence":"first","affiliation":[{"name":"Inserm UMR-S1250, P3Cell, University of Reims Champagne-Ardenne, SFR CAP-SANTE, 51092 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanne-Marie","family":"Perotin","sequence":"additional","affiliation":[{"name":"Inserm UMR-S1250, P3Cell, University of Reims Champagne-Ardenne, SFR CAP-SANTE, 51092 Reims, France"},{"name":"Department of Respiratory Diseases, Centre Hospitalier Universitaire de Reims, H\u00f4pital Maison Blanche, 51092 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randa","family":"Belgacemi","sequence":"additional","affiliation":[{"name":"Inserm UMR-S1250, P3Cell, University of Reims Champagne-Ardenne, SFR CAP-SANTE, 51092 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3128-9348","authenticated-orcid":false,"given":"Julien","family":"Ancel","sequence":"additional","affiliation":[{"name":"Inserm UMR-S1250, P3Cell, University of Reims Champagne-Ardenne, SFR CAP-SANTE, 51092 Reims, France"},{"name":"Department of Respiratory Diseases, Centre Hospitalier Universitaire de Reims, H\u00f4pital Maison Blanche, 51092 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Mulette","sequence":"additional","affiliation":[{"name":"Inserm UMR-S1250, P3Cell, University of Reims Champagne-Ardenne, SFR CAP-SANTE, 51092 Reims, France"},{"name":"Department of Respiratory Diseases, Centre Hospitalier Universitaire de Reims, H\u00f4pital Maison Blanche, 51092 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonzague","family":"Delepine","sequence":"additional","affiliation":[{"name":"Inserm UMR-S1250, P3Cell, University of Reims Champagne-Ardenne, SFR CAP-SANTE, 51092 Reims, France"},{"name":"Department of Thoracic Surgery, Centre Hospitalier Universitaire de Reims, H\u00f4pital Maison Blanche, 51092 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4043-6429","authenticated-orcid":false,"given":"Philippe","family":"Gosset","sequence":"additional","affiliation":[{"name":"CNRS UMR9017, Inserm U1019, University of Lille, Centre Hospitalier R\u00e9gional Universitaire de Lille, Institut Pasteur, CIIL\u2014Center for Infection and Immunity of Lille, 59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe","family":"Maskos","sequence":"additional","affiliation":[{"name":"Integrative Neurobiology of Cholinergic Systems, Institut Pasteur, CNRS UMR 3571, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myriam","family":"Polette","sequence":"additional","affiliation":[{"name":"Inserm UMR-S1250, P3Cell, University of Reims Champagne-Ardenne, SFR CAP-SANTE, 51092 Reims, France"},{"name":"Department of Biopathology, Centre Hospitalier Universitaire de Reims, H\u00f4pital Maison Blanche, 51092 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ga\u00ebtan","family":"Desl\u00e9e","sequence":"additional","affiliation":[{"name":"Inserm UMR-S1250, P3Cell, University of Reims Champagne-Ardenne, SFR CAP-SANTE, 51092 Reims, France"},{"name":"Department of Respiratory Diseases, Centre Hospitalier Universitaire de Reims, H\u00f4pital Maison Blanche, 51092 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1725-371X","authenticated-orcid":false,"given":"Val\u00e9rian","family":"Dormoy","sequence":"additional","affiliation":[{"name":"Inserm UMR-S1250, P3Cell, University of Reims Champagne-Ardenne, SFR CAP-SANTE, 51092 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"338","DOI":"10.2174\/1570159X15666170912110450","article-title":"Neuronal and Extraneuronal Nicotinic Acetylcholine Receptors","volume":"16","author":"Zoli","year":"2018","journal-title":"Curr. 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