{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T22:44:47Z","timestamp":1776379487356,"version":"3.51.2"},"reference-count":21,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T00:00:00Z","timestamp":1602720000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>(1) Background: Circulating micro-RNAs (miRNAs) modulate the expression of molecules in diabetes. We evaluated the expression of serum miRNA-195-5p and -451a in diabetic patients with ischemic stroke and correlated them with two markers of brain tissue integrity. (2) Methods: Seventy-eight subjects with acute ischemic stroke (AIS) or transient ischemic attack (TIA) (40 with diabetes) were enrolled. Serum miRNA levels, brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor A (VEGF-A) were assessed at admission and 24 and 72 h after a post-ischemic stroke, and were compared to 20 controls. (3) Results: Both circulating miRNAs were two-fold up-regulated in diabetic AIS and TIA patients compared to non-diabetics. Their levels progressively decreased at 24 and 72 h in both AIS and TIA patients. Interestingly, in the non-diabetic TIA group, both circulating miRNAs, although higher than the controls, tended to achieve a complete decay after 72 h. Furthermore, miRNA-195-5p and miRNA-451a levels inversely correlated with both BDNF and VEGF-A serum levels. (4) Conclusions: These data show a different profile of both micro-RNAs in diabetic versus non-diabetic patients after acute ischemic stroke, suggesting their pivotal role in cerebrovascular ischemic attack.<\/jats:p>","DOI":"10.3390\/ijms21207615","type":"journal-article","created":{"date-parts":[[2020,10,15]],"date-time":"2020-10-15T09:02:03Z","timestamp":1602752523000},"page":"7615","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Circulating MiRNA-195-5p and -451a in Diabetic Patients with Transient and Acute Ischemic Stroke in the Emergency Department"],"prefix":"10.3390","volume":"21","author":[{"given":"Mauro","family":"Giordano","sequence":"first","affiliation":[{"name":"Department of Advanced Medical and Surgical Sciences, University of Campania \u201cL. Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9813-5955","authenticated-orcid":false,"given":"Maria Consiglia","family":"Trotta","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, Division of Pharmacology, University of Campania \u201cL. Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiziana","family":"Ciarambino","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Hospital of Marcianise, ASL, 81025 Caserta, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6899-0595","authenticated-orcid":false,"given":"Michele","family":"D\u2019Amico","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, Division of Pharmacology, University of Campania \u201cL. Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilena","family":"Galdiero","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, Division of Pharmacology, University of Campania \u201cL. Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Schettini","sequence":"additional","affiliation":[{"name":"Department of Advanced Medical and Surgical Sciences, University of Campania \u201cL. Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Paternosto","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, AORN Sant\u2019Anna e San Sebastiano,81100 Caserta, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Salzillo","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, AORN Sant\u2019Anna e San Sebastiano,81100 Caserta, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Alfano","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, Division of Pharmacology, University of Campania \u201cL. Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincenzo","family":"Andreone","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, AORN Sant\u2019Anna e San Sebastiano,81100 Caserta, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2944-2729","authenticated-orcid":false,"given":"Lorenzo Salvatore","family":"Malatino","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, University of Catania, 95126 Catania, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianni","family":"Biolo","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, University of Trieste, 34100 Trieste, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Paolisso","sequence":"additional","affiliation":[{"name":"Department of Advanced Medical and Surgical Sciences, University of Campania \u201cL. Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8453-4912","authenticated-orcid":false,"given":"Luigi Elio","family":"Adinolfi","sequence":"additional","affiliation":[{"name":"Department of Advanced Medical and Surgical Sciences, University of Campania \u201cL. Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.pneurobio.2013.11.004","article-title":"Vascular remodeling after ischemic stroke: Mechanisms and therapeutic potentials","volume":"115","author":"Liu","year":"2014","journal-title":"Prog. Neurobiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"352","DOI":"10.2174\/15701611113119990016","article-title":"Angiogenesis-regulating microRNAs and Ischemic Stroke","volume":"13","author":"Yin","year":"2015","journal-title":"Curr. Vasc. Pharmacol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Chen, Z., Lai, T.C., Jan, Y.H., Lin, F.M., Wang, W.C., Xiao, H., Wang, Y.T., Sun, W., Cui, X., and Li, Y.S. (2013). Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis. J. Clin. Investig.","DOI":"10.1172\/JCI65344"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.3892\/or.2016.4971","article-title":"miR-451 acts as a suppressor of angiogenesis in hepatocellular carcinoma by targeting the IL-6R-STAT3 pathway","volume":"36","author":"Liu","year":"2016","journal-title":"Oncol. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"5434","DOI":"10.3892\/mmr.2017.7230","article-title":"Downregulation of microRNA-195 promotes Angiogenesis induced by cerebral infarction via targeting VEGFA","volume":"16","author":"Zhao","year":"2017","journal-title":"Mol. Med. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Giordano, M., Ciarambino, T., D\u2019Amico, M., Trotta, M., Di Sette, A., Marfella, R., Malatino, L., Paolisso, G., and Adinolfi, L. (2019). Circulating MiRNA-195-5p and -451a in Transient and Acute Ischemic Stroke Patients in an Emergency Department. J. Clin. Med., 8.","DOI":"10.3390\/jcm8020130"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Seto, S.-W., Chang, D., Jenkins, A., Bensoussan, A., and Kiat, H. (2016). Angiogenesis in Ischemic Stroke and Angiogenic Effects of Chinese Herbal Medicine. J. Clin. Med., 5.","DOI":"10.3390\/jcm5060056"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Chen, R., Ovbiagele, B., and Feng, W. (2016). Diabetes and Stroke: Epidemiology, Pathophysiology, Pharmaceuticals and Outcomes. Am. J. Med. Sci.","DOI":"10.1016\/j.amjms.2016.01.011"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s12974-016-0774-5","article-title":"Cerebral ischemic damage in diabetes: An inflammatory perspective","volume":"14","author":"Shukla","year":"2017","journal-title":"J. Neuroinflamm."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Tuttolomondo, A., Maida, C., and Pinto, A. (2015). Diabetic foot syndrome: Immune-inflammatory features as possible cardiovascular markers in diabetes. World J. Orthop.","DOI":"10.1155\/2015\/268390"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Di Raimondo, D., Tuttolomondo, A., Butt\u00e0, C., Casuccio, A., Giarrusso, L., Miceli, G., Licata, G., and Pinto, A. (2013). Metabolic and anti-inflammatory effects of a home-based programme of aerobic physical exercise. Int. J. Clin. Pract.","DOI":"10.1111\/ijcp.12269"},{"key":"ref_12","first-page":"261","article-title":"Risk factors profile and clinical outcome of ischemic stroke patients admitted in a Department of Internal Medicine and classified by TOAST classification","volume":"25","author":"Pinto","year":"2006","journal-title":"Int. Angiol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Tuttolomondo, A., Di Raimondo, D., Pecoraro, R., Casuccio, A., Di Bona, D., Aiello, A., Accardi, G., Arnao, V., Clemente, G., and Corte, V. (2019). HLA and killer cell immunoglobulin-like receptor (KIRs) genotyping in patients with acute ischemic stroke. J. Neuroinflamm.","DOI":"10.1186\/s12974-019-1469-5"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"117906951983228","DOI":"10.1177\/1179069519832286","article-title":"A Review of MicroRNA Biomarkers in Traumatic Brain Injury","volume":"13","author":"Atif","year":"2019","journal-title":"J. Exp. Neurosci."},{"key":"ref_15","first-page":"298","article-title":"Angiogenesis, neurogenesis and brain recovery of function following injury","volume":"11","author":"Xiong","year":"2010","journal-title":"Curr. Opin. Investig. Drugs"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2270","DOI":"10.1161\/STROKEAHA.111.642710","article-title":"Angiogenesis: A harmonized target for recovery after stroke","volume":"43","author":"Ergul","year":"2012","journal-title":"Stroke"},{"key":"ref_17","first-page":"1327","article-title":"International Expert Committee Report on the Role of the A1C Assay in the Diagnosis of Diabetes","volume":"32","author":"Gillett","year":"2009","journal-title":"Clin. Biochem. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2276","DOI":"10.1161\/STROKEAHA.108.192218","article-title":"Definition and evaluation of transient ischemic attack: A scientific statement for health care professionals from the American Heart Association\/American Stroke Association Stroke Council; Council on Cardiovascular Surgery and Anesthesia; Council on Cardiovascular Radiology and Intervention; Council on Cardiovascular Nursing; and the Interdisciplinary Council on Peripheral Vascular Disease. The American Academy of Neurology affirms the value of this statement as an educational tool for neurologists","volume":"40","author":"Easton","year":"2009","journal-title":"Stroke"},{"key":"ref_19","first-page":"e277","article-title":"2018 Guidelines for the Early Management of Patients With Acute Ischemic Stroke: A Guideline for Healthcare Professionals From the American Heart Association\/American Stroke Association","volume":"50","author":"Powers","year":"2018","journal-title":"Stroke"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1093\/eurjhf\/hft088","article-title":"CirculatingmicroRNA changes in heart failure patients treated with cardiac resynchronization therapy: Responders vs. non-responders","volume":"15","author":"Marfella","year":"2013","journal-title":"Eur. J. Heart Fail."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Eyileten, C., Wicik, Z., De Rosa, S., Mirowska-Guzel, D., Soplinska, A., Indolfi, C., Jastrzebska-Kurkowska, I., Czlonkowska, A., and Postula, M. (2018). MicroRNAs as Diagnostic and Prognostic Biomarkers in Ischemic Stroke\u2014A Comprehensive Review and Bioinformatic Analysis. Cells, 7.","DOI":"10.3390\/cells7120249"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/20\/7615\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:21:32Z","timestamp":1760178092000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/20\/7615"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,15]]},"references-count":21,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2020,10]]}},"alternative-id":["ijms21207615"],"URL":"https:\/\/doi.org\/10.3390\/ijms21207615","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,15]]}}}