{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:47:44Z","timestamp":1791499664591,"version":"4.3.4"},"reference-count":86,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T00:00:00Z","timestamp":1602806400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1160139"],"award-info":[{"award-number":["1160139"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["IJMS"],"abstract":"<jats:p>Nerve Growth Factor (NGF) and its high-affinity receptor tropomyosin receptor kinase A (TRKA) increase their expression during the progression of epithelial ovarian cancer (EOC), promoting cell proliferation and angiogenesis through several oncogenic proteins, such as c-MYC and vascular endothelial growth factor (VEGF). The expression of these proteins is controlled by microRNAs (miRs), such as miR-145, whose dysregulation has been related to cancer. The aims of this work were to evaluate in EOC cells whether NGF\/TRKA decreases miR-145 levels, and the effect of miR-145 upregulation. The levels of miR-145-5p were assessed by qPCR in ovarian biopsies and ovarian cell lines (human ovarian surface epithelial cells (HOSE), A2780 and SKOV3) stimulated with NGF. Overexpression of miR-145 in ovarian cells was used to evaluate cell proliferation, migration, invasion, c-MYC and VEGF protein levels, as well as tumor formation and metastasis in vivo. In EOC samples, miR-145-5p levels were lower than in epithelial ovarian tumors. Overexpression of miR-145 decreased cell proliferation, migration and invasion of EOC cells, changes that were concomitant with the decrease in c-MYC and VEGF protein levels. We observed decreased tumor formation and suppressed metastasis behavior in mice injected with EOC cells that overexpressed miR-145. As expected, ovarian cell lines stimulated with NGF diminished miR-145-5p transcription and abundance. These results suggest that the tumoral effects of NGF\/TRKA depend on the regulation of miR-145-5p levels in EOC cells, and that its upregulation could be used as a possible therapeutic strategy for EOC.<\/jats:p>","DOI":"10.3390\/ijms21207657","type":"journal-article","created":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T08:56:48Z","timestamp":1602838608000},"page":"7657","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["NGF\/TRKA Decrease miR-145-5p Levels in Epithelial Ovarian Cancer Cells"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7234-3916","authenticated-orcid":false,"given":"Maritza P.","family":"Garrido","sequence":"first","affiliation":[{"name":"Laboratorio de Endocrinolog\u00eda y Biolog\u00eda de la Reproducci\u00f3n, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"},{"name":"Departamento de Obstetricia y Ginecolog\u00eda, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Torres","sequence":"additional","affiliation":[{"name":"Laboratorio de Endocrinolog\u00eda y Biolog\u00eda de la Reproducci\u00f3n, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Avila","sequence":"additional","affiliation":[{"name":"Centro de Medicina Regenerativa, Facultad de Medicina, Cl\u00ednica Alemana-Universidad del Desarrollo, Santiago 7710162, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2541-523X","authenticated-orcid":false,"given":"Jon\u00e1s","family":"Chnaiderman","sequence":"additional","affiliation":[{"name":"Programa de Virolog\u00eda, Instituto de Ciencias Biom\u00e9dicas, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8833-7382","authenticated-orcid":false,"given":"Manuel","family":"Valenzuela-Valderrama","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Celular. Instituto de Investigaci\u00f3n e Innovaci\u00f3n en Salud. Facultad de Ciencias de la Salud, Universidad Central de Chile, Santiago 8320000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Aramburo","sequence":"additional","affiliation":[{"name":"Laboratorio de Endocrinolog\u00eda y Biolog\u00eda de la Reproducci\u00f3n, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9563-4318","authenticated-orcid":false,"given":"Lorena","family":"Or\u00f3stica","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica (CIB), Facultad de Medicina, Universidad Diego Portales, Santiago 8370007, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6974-7004","authenticated-orcid":false,"given":"Eduardo","family":"Dur\u00e1n-Jara","sequence":"additional","affiliation":[{"name":"Centro de Medicina Regenerativa, Facultad de Medicina, Cl\u00ednica Alemana-Universidad del Desarrollo, Santiago 7710162, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Lobos-Gonzalez","sequence":"additional","affiliation":[{"name":"Centro de Medicina Regenerativa, Facultad de Medicina, Cl\u00ednica Alemana-Universidad del Desarrollo, Santiago 7710162, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Romero","sequence":"additional","affiliation":[{"name":"Laboratorio de Endocrinolog\u00eda y Biolog\u00eda de la Reproducci\u00f3n, Hospital Cl\u00ednico Universidad de Chile, Santiago 8380456, Chile"},{"name":"Departamento de Obstetricia y Ginecolog\u00eda, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,16]]},"reference":[{"key":"ref_1","first-page":"69","article-title":"Global cancer statistics","volume":"61","author":"Jemal","year":"2011","journal-title":"CA Cancer J. 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