{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T15:26:11Z","timestamp":1775057171163,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2020,10,24]],"date-time":"2020-10-24T00:00:00Z","timestamp":1603497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Fi1700\/5-1; Fi1700\/7-1"],"award-info":[{"award-number":["Fi1700\/5-1; Fi1700\/7-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG TE 912\/2-2"],"award-info":[{"award-number":["DFG TE 912\/2-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020","doi-asserted-by":"publisher","award":["ADIPOA2 643809; Orthounion 733288"],"award-info":[{"award-number":["ADIPOA2 643809; Orthounion 733288"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Brown adipose tissue (BAT) is a thermogenic organ in rodents and humans. In mice, the transplantation of BAT has been successfully used to combat obesity and its comorbidities. While such beneficial properties of BAT are now evident, the developmental and cellular origins of brown, beige, and white adipocytes have remained only poorly understood, especially in humans. We recently discovered that CD90 is highly expressed in stromal cells isolated from human white adipose tissue (WAT) compared to BAT. Here, we studied whether CD90 interferes with brown or white adipogenesis or white adipocyte beiging. We applied flow cytometric sorting of human adipose tissue stromal cells (ASCs), a CRISPR\/Cas9 knockout strategy in the human Simpson-Golabi-Behmel syndrome (SGBS) adipocyte model system, as well as a siRNA approach in human approaches supports the hypothesis that CD90 affects brown or white adipogenesis or white adipocyte beiging in humans. Taken together, our findings call the conclusions drawn from previous studies, which claimed a central role of CD90 in adipocyte differentiation, into question.<\/jats:p>","DOI":"10.3390\/ijms21217907","type":"journal-article","created":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T02:34:54Z","timestamp":1603679694000},"page":"7907","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["CD90 Is Dispensable for White and Beige\/Brown Adipocyte Differentiation"],"prefix":"10.3390","volume":"21","author":[{"given":"Meike","family":"Dahlhaus","sequence":"first","affiliation":[{"name":"Division of Pediatric Endocrinology and Diabetes, Ulm University Medical Center, 89075 Ulm, Germany"},{"name":"Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, 89075 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2105-6671","authenticated-orcid":false,"given":"Julian","family":"Roos","sequence":"additional","affiliation":[{"name":"Division of Pediatric Endocrinology and Diabetes, Ulm University Medical Center, 89075 Ulm, Germany"},{"name":"Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, 89075 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Engel","sequence":"additional","affiliation":[{"name":"Division of Pediatric Endocrinology and Diabetes, Ulm University Medical Center, 89075 Ulm, Germany"},{"name":"Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, 89075 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Tews","sequence":"additional","affiliation":[{"name":"Division of Pediatric Endocrinology and Diabetes, Ulm University Medical Center, 89075 Ulm, Germany"},{"name":"Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, 89075 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Halbgebauer","sequence":"additional","affiliation":[{"name":"Division of Pediatric Endocrinology and Diabetes, Ulm University Medical Center, 89075 Ulm, Germany"},{"name":"Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, 89075 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan-Bernd","family":"Funcke","sequence":"additional","affiliation":[{"name":"Division of Pediatric Endocrinology and Diabetes, Ulm University Medical Center, 89075 Ulm, Germany"},{"name":"Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, 89075 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Kiener","sequence":"additional","affiliation":[{"name":"Division of Pediatric Endocrinology and Diabetes, Ulm University Medical Center, 89075 Ulm, Germany"},{"name":"Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, 89075 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1065-8709","authenticated-orcid":false,"given":"Patrick J.","family":"Schuler","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Centre, 89075 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes","family":"D\u00f6scher","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Centre, 89075 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas K.","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery, Ulm University Medical Centre, 89075 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Zinngrebe","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, 89075 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Rojewski","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine, University of Ulm, 89081 Ulm, Germany"},{"name":"Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service, Baden Wuerttemberg-Hessen, and University Hospital Ulm, 89081 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hubert","family":"Schrezenmeier","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine, University of Ulm, 89081 Ulm, Germany"},{"name":"Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service, Baden Wuerttemberg-Hessen, and University Hospital Ulm, 89081 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus-Michael","family":"Debatin","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, 89075 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Wabitsch","sequence":"additional","affiliation":[{"name":"Division of Pediatric Endocrinology and Diabetes, Ulm University Medical Center, 89075 Ulm, Germany"},{"name":"Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, 89075 Ulm, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3402-9840","authenticated-orcid":false,"given":"Pamela","family":"Fischer-Posovszky","sequence":"additional","affiliation":[{"name":"Division of Pediatric Endocrinology and Diabetes, Ulm 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