{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T06:41:02Z","timestamp":1762324862602,"version":"build-2065373602"},"reference-count":67,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T00:00:00Z","timestamp":1603929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CICYT-FEDER","award":["AGL 2017-83799-R","AGL 2013-43328-P"],"award-info":[{"award-number":["AGL 2017-83799-R","AGL 2013-43328-P"]}]},{"DOI":"10.13039\/501100006021","name":"DGA","doi-asserted-by":"publisher","award":["A-07_17R"],"award-info":[{"award-number":["A-07_17R"]}],"id":[{"id":"10.13039\/501100006021","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007637","name":"Colciencias","doi-asserted-by":"publisher","award":["Code Research Program 110157635854, contract 0607-2013"],"award-info":[{"award-number":["Code Research Program 110157635854, contract 0607-2013"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This work aimed to determine the contribution of the testis and epididymis and the effect of the photoperiodic regimen on ram seminal plasma (SP). Semen was collected from 15 mature rams located in an equatorial (Colombian Creole and Romney Marsh, eight intact and two vasectomized) or a temperate climate (Rasa Aragonesa, three intact and two vasectomized). SP proteins were analyzed by Bradford, SDS-PAGE and difference gel electrophoresis (DIGE). Melatonin and testosterone concentrations were quantified by ELISA, and activity of glutathione peroxidase (GPx), glutathione reductase (GRD), and catalase by enzymatic assays. Vasectomy increased protein concentration and the intensity of high molecular weight bands (p &lt; 0.001), with no differences between breeds. DIGE revealed the absence of six proteins in vasectomized rams: angiotensin-converting enzyme, lactotransferrin, phosphoglycerate kinase, sorbitol dehydrogenase, epididymal secretory glutathione peroxidase and epididymal secretory protein E1. Vasectomy also decreased melatonin concentrations in seasonal rams, and testosterone in all of them (p &lt; 0.001), but did not affect antioxidant enzyme activity. Equatorial rams showed lower melatonin and testosterone concentration (p &lt; 0.01) and catalase, but higher GPx activity (p &lt; 0.05). In conclusion, vasectomy modifies the protein profile and hormonal content of ram seminal plasma, whereas the exposure to a constant photoperiod affects hormonal concentration and antioxidant enzymes activity.<\/jats:p>","DOI":"10.3390\/ijms21218063","type":"journal-article","created":{"date-parts":[[2020,10,29]],"date-time":"2020-10-29T09:44:53Z","timestamp":1603964693000},"page":"8063","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Vasectomy and Photoperiodic Regimen Modify the Protein Profile, Hormonal Content and Antioxidant Enzymes Activity of Ram Seminal Plasma"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3929-5064","authenticated-orcid":false,"given":"Melissa","family":"Carvajal-Serna","sequence":"first","affiliation":[{"name":"Grupo BIOFITER (Biolog\u00eda, Fisiolog\u00eda y Tecnolog\u00edas de la Reproducci\u00f3n), Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular, Instituto Universitario de Investigaci\u00f3n en Ciencias Ambientales de Arag\u00f3n (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain"},{"name":"Departamento de Producci\u00f3n Animal, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogot\u00e1 111321, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meriem","family":"Fatnassi","sequence":"additional","affiliation":[{"name":"Livestock and Wildlife Laboratory, Arid Lands Institute, University of Gab\u00e8s, M\u00e9denine 6029, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felipe","family":"Torres-Ruda","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogot\u00e1 111321, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7744-2881","authenticated-orcid":false,"given":"Jaime Antonio","family":"Cardozo","sequence":"additional","affiliation":[{"name":"Researcher Center Tibaitat\u00e1, AGROSAVIA (Corporaci\u00f3n Colombiana de Investigaci\u00f3n Agropecuaria), Bogot\u00e1 250047, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry","family":"Grajales-Lombana","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogot\u00e1 111321, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Hammadi","sequence":"additional","affiliation":[{"name":"Livestock and Wildlife Laboratory, Arid Lands Institute, University of Gab\u00e8s, M\u00e9denine 6029, Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2827-3054","authenticated-orcid":false,"given":"Jose Alfonso","family":"Abecia","sequence":"additional","affiliation":[{"name":"Grupo BIOFITER (Biolog\u00eda, Fisiolog\u00eda y Tecnolog\u00edas de la Reproducci\u00f3n), Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular, Instituto Universitario de Investigaci\u00f3n en Ciencias Ambientales de Arag\u00f3n (IUCA), Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Mui\u00f1o-Blanco","sequence":"additional","affiliation":[{"name":"Grupo BIOFITER (Biolog\u00eda, Fisiolog\u00eda y Tecnolog\u00edas de la Reproducci\u00f3n), Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular, Instituto Universitario de Investigaci\u00f3n en Ciencias Ambientales de Arag\u00f3n (IUCA), Facultad de Veterinaria, Universidad 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