{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:56:56Z","timestamp":1761897416982,"version":"build-2065373602"},"reference-count":46,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2020,11,12]],"date-time":"2020-11-12T00:00:00Z","timestamp":1605139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003668","name":"Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries","doi-asserted-by":"publisher","award":["MAFRA 316027-5"],"award-info":[{"award-number":["MAFRA 316027-5"]}],"id":[{"id":"10.13039\/501100003668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The aim of this study was to identify an anti-obesity peptide from Allomyrina dichotoma and investigate the lipid metabolic mechanism. Enzymatically hydrolyzed A. dichotoma larvae were further separated using tangential flow filtration and consecutive chromatographic processes. Finally, an anti-obesity peptide that showed the highest inhibitory effect on lipid accumulation was obtained, and the sequence was Glu-Ile-Ala-Gln-Asp-Phe-Lys-Thr-Asp-Leu (EIA10). EIA10 decreased lipid aggregation in vitro and significantly reduced the accumulation of body weight gain, liver weight, and adipose tissue weight in high-fat-fed mice. Compared with the control group, the levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL), insulin, and homeostasis model assessment of insulin resistance (HOMA-IR) in the high-fat diet (HFD) group increased significantly, and the content of high-density lipoprotein cholesterol (HDL) in the serum decreased significantly. On the contrary, the levels of TC, TG, and insulin in the EIA10 group decreased significantly, and the HDL content increased significantly compared with the HFD group. Additionally, EIA10 dramatically decreased mRNA and protein levels of transcription factors involved in lipid adipogenesis. Taken together, our results suggest that EIA10 could be a promising agent for the treatment and prevention of obesity.<\/jats:p>","DOI":"10.3390\/ijms21228537","type":"journal-article","created":{"date-parts":[[2020,11,12]],"date-time":"2020-11-12T10:00:32Z","timestamp":1605175232000},"page":"8537","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Exploring the Role of a Novel Peptide from Allomyrina dichotoma Larvae in Ameliorating Lipid Metabolism in Obesity"],"prefix":"10.3390","volume":"21","author":[{"given":"Sung Mun","family":"Bae","sequence":"first","affiliation":[{"name":"Gyeongnam Agricultural Research and Extension Services, Jinju 52733, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiqi","family":"Fan","sequence":"additional","affiliation":[{"name":"Division of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young-Jin","family":"Choi","sequence":"additional","affiliation":[{"name":"Division of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujiao","family":"Tang","sequence":"additional","affiliation":[{"name":"Division of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, Chungju 27478, Korea"},{"name":"School of Bio-Science and Food Engineering, Changchun University of Science and Technology, Changchun 130600, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gwanghui","family":"Jeong","sequence":"additional","affiliation":[{"name":"Gyeongnam Agricultural Research and Extension Services, Jinju 52733, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyungjae","family":"Myung","sequence":"additional","affiliation":[{"name":"Center for Genomic Integrity, Institute for Basic Science, Ulsan 44919, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1152-4542","authenticated-orcid":false,"given":"Byung-gyu","family":"Kim","sequence":"additional","affiliation":[{"name":"Center for Genomic Integrity, Institute for Basic Science, Ulsan 44919, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4832-6427","authenticated-orcid":false,"given":"Eun-Kyung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Dong-A University, Busan 49315, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.foodchem.2014.10.114","article-title":"Review of food composition data for edible insects","volume":"193","author":"Nowak","year":"2016","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1093\/fqsafe\/fyy001","article-title":"Edible insects as a means to address global malnutrition and food insecurity issues","volume":"2","author":"Tao","year":"2018","journal-title":"Food Qual. Saf."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1292\/jvms.66.137","article-title":"Effect of modified oligopeptides from the beetle Allomyrina dichotoma on Escherichia coli infection in mice","volume":"66","author":"Yamada","year":"2004","journal-title":"J. Vet. Med. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"319","DOI":"10.3844\/ajabssp.2009.319.331","article-title":"The yellow mealworm as a novel source of protein","volume":"4","author":"Ghaly","year":"2009","journal-title":"Am. J. Agric. Biol. Sci."},{"key":"ref_5","first-page":"349","article-title":"Allomyrina dichotoma larva extracts protect streptozotocin-induced oxidative cytotoxicity","volume":"22","author":"Kim","year":"1986","journal-title":"J. Environ. Toxicol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/1748-5967.12044","article-title":"Anti-obesity effect of Allomyrina dichotoma (A rthropoda: Insecta) larvae ethanol extract on 3T3-L1 adipocyte differentiation","volume":"44","author":"Chung","year":"2014","journal-title":"Entomol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1111\/1748-5967.12140","article-title":"Exosome isolation from hemolymph of K orean rhinoceros beetle, Allomyrina dichotoma (Coleoptera: Scarabaeidae)","volume":"45","author":"Kim","year":"2015","journal-title":"Entomol. Res."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Kim, J., Yun, E.Y., Park, S.W., Goo, T.W., and Seo, M. (2016). Allomyrina dichotoma larvae regulate food intake and body weight in high fat diet-induced obese mice through mTOR and Mapk signaling pathways. Nutrients, 8.","DOI":"10.3390\/nu8020100"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Hu, F., Zhang, Y., Song, Y., and Baez, R.V. (2013). Lipid metabolism, metabolic syndrome, and cancer. Lipid Metab., 185\u2013210.","DOI":"10.5772\/51821"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"498","DOI":"10.3390\/nu5020498","article-title":"Fat depots, free fatty acids, and dyslipidemia","volume":"5","author":"Ebbert","year":"2013","journal-title":"Nutrients"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11427-019-1563-3","article-title":"Regulation of glucose and lipid metabolism in health and disease","volume":"62","author":"Chen","year":"2019","journal-title":"Sci. China Life Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e13048","DOI":"10.1111\/acel.13048","article-title":"The role of lipid metabolism in aging, lifespan regulation, and age-related disease","volume":"18","author":"Johnson","year":"2019","journal-title":"Aging Cell"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.metabol.2018.12.012","article-title":"The pathogenesis of obesity","volume":"92","author":"Oussaada","year":"2019","journal-title":"Metabolism"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1016\/j.fertnstert.2017.02.104","article-title":"Obesity pandemic: Causes, consequences, and solutions\u2014But do we have the will?","volume":"107","author":"Meldrum","year":"2017","journal-title":"Fertil. Steril."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1007\/s40264-014-0210-7","article-title":"Benefit-risk assessment of orlistat in the treatment of obesity","volume":"37","author":"Sumithran","year":"2014","journal-title":"Drug Saf."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"111","DOI":"10.3177\/jnsv.63.111","article-title":"Egg white hydrolysate can be a low-allergenic food material to suppress ectopic fat accumulation in rats fed an equicaloric diet","volume":"63","author":"Ochiai","year":"2017","journal-title":"J. Nutr. Sci. Vitaminol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Garc\u00e9s-Rim\u00f3n, M., Gonz\u00e1lez, C., Uranga, J.A., L\u00f3pez-Miranda, V., L\u00f3pez-Fandi\u00f1o, R., and Miguel, M. (2016). Pepsin egg white hydrolysate ameliorates obesity-related oxidative stress, inflammation and steatosis in Zucker Fatty Rats. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0151193"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"C de Campos Zani, S., Wu, J., and B Chan, C. (2018). Egg and soy-derived peptides and hydrolysates: A review of their physiological actions against diabetes and obesity. Nutrients, 10.","DOI":"10.3390\/nu10050549"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.foodchem.2018.03.082","article-title":"Characterization and identification of novel antidiabetic and anti-obesity peptides from camel milk protein hydrolysates","volume":"259","author":"Mudgil","year":"2018","journal-title":"Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.ijbiomac.2017.05.151","article-title":"Fish collagen peptide inhibits the adipogenic differentiation of preadipocytes and ameliorates obesity in high fat diet-fed mice","volume":"104","author":"Lee","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"77","DOI":"10.2298\/ABS170401027R","article-title":"Prevention of diet-induced obesity in rats by oral application of collagen fragments","volume":"70","author":"Raksha","year":"2018","journal-title":"Arch. Biol. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2907","DOI":"10.1172\/JCI107115","article-title":"Effect of age, sex, and site on the cellularity of the adipose tissue in mice and rats rendered obese by a high-fat diet","volume":"51","author":"Lemonnier","year":"1972","journal-title":"J. Clin. Investig."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Liou, C.J., Lee, Y.K., Ting, N.C., Chen, Y.L., Shen, S.C., Wu, S.J., and Huang, W.C. (2019). Protective effects of Licochalcone A ameliorates obesity and non-alcoholic fatty liver disease via promotion of the Sirt-1\/AMPK pathway in mice fed a high-fat diet. Cells, 8.","DOI":"10.3390\/cells8050447"},{"key":"ref_24","first-page":"245","article-title":"The effect of adropin on lipid and glucose metabolism in rats with hyperlipidemia","volume":"19","author":"Bayat","year":"2016","journal-title":"Iran. J. Basic Med. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12933-019-0954-6","article-title":"Liraglutide improves lipid metabolism by enhancing cholesterol efflux associated with ABCA1 and ERK1\/2 pathway","volume":"18","author":"Wu","year":"2019","journal-title":"Cardiovasc. Diabetol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jff.2019.03.043","article-title":"Polysaccharide peptides from Ganoderma lucidum ameliorate lipid metabolic disorders and gut microbiota dysbiosis in high-fat diet-fed rats","volume":"57","author":"Lv","year":"2019","journal-title":"J. Funct. Foods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2073","DOI":"10.1194\/jlr.P068585","article-title":"Statin action enriches HDL3 in polyunsaturated phospholipids and plasmalogens and reduces LDL-derived phospholipid hydroperoxides in atherogenic mixed dyslipidemia","volume":"57","author":"Orsoni","year":"2016","journal-title":"J. Lipid Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2663","DOI":"10.1021\/jf1043508","article-title":"Mulberry water extracts possess an anti-obesity effect and ability to inhibit hepatic lipogenesis and promote lipolysis","volume":"59","author":"Peng","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","first-page":"1","article-title":"Triglyceride metabolism in the liver","volume":"8","author":"Cohen","year":"2011","journal-title":"Compr. Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2133","DOI":"10.1152\/physrev.00063.2017","article-title":"Mechanisms of Insulin Action and Insulin Resistance","volume":"98","author":"Petersen","year":"2018","journal-title":"Physiol. Rev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/s12967-015-0412-5","article-title":"Caulerpa lentillifera extract ameliorates insulin resistance and regulates glucose metabolism in C57BL\/KsJ-db\/db mice via PI3K\/AKT signaling pathway in myocytes","volume":"13","author":"Sharma","year":"2015","journal-title":"J. Transl. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1016\/j.cmet.2011.03.009","article-title":"AMPK phosphorylates and inhibits SREBP activity to attenuate hepatic steatosis and atherosclerosis in diet-induced insulin-resistant mice","volume":"13","author":"Li","year":"2011","journal-title":"Cell Metab."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"332","DOI":"10.14218\/JCTH.2017.00061","article-title":"Role of SREBPs in liver diseases: A mini-review","volume":"6","author":"Moslehi","year":"2018","journal-title":"J. Clin. Transl. Hepatol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Gu, S.H., Wu, K.M., Guo, Y.Y., Pickett, J.A., Field, L.M., Zhou, J.J., and Zhang, Y.J. (2013). Identification of genes expressed in the sex pheromone gland of the black cutworm Agrotis ipsilon with putative roles in sex pheromone biosynthesis and transport. BMC Genomics, 14.","DOI":"10.1186\/1471-2164-14-636"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"902","DOI":"10.3389\/fphys.2017.00902","article-title":"Palmitic acid: Physiological role, metabolism and nutritional implications","volume":"8","author":"Carta","year":"2017","journal-title":"Front. Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1128\/MCB.01344-13","article-title":"Peroxisome proliferator-activated receptor \u03b3 and C\/EBP\u03b1 synergistically activate key metabolic adipocyte genes by assisted loading","volume":"34","author":"Madsen","year":"2014","journal-title":"Mol. Cell. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Wang, Y., Nakajima, T., Gonzalez, F.J., and Tanaka, N. (2020). PPARs as Metabolic Regulators in the Liver: Lessons from Liver-Specific PPAR-Null Mice. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21062061"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"6666","DOI":"10.1021\/jf050582+","article-title":"Reactive oxygen scavenging effect of enzymatic extracts from Sargassum thunbergii","volume":"53","author":"Park","year":"2005","journal-title":"J. Agr. Food Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.2337\/diabetes.47.10.1562","article-title":"Prolonged oxidative stress impairs insulin-induced GLUT4 translocation in 3T3-L1 adipocytes","volume":"47","author":"Rudich","year":"1998","journal-title":"Diabetes"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"5383","DOI":"10.1021\/ac025747h","article-title":"Empirical statistical model to estimate the accuracy of peptide identifications made by MS\/MS and database search","volume":"74","author":"Keller","year":"2002","journal-title":"Anal. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4646","DOI":"10.1021\/ac0341261","article-title":"A statistical model for identifying proteins by tandem mass spectrometry","volume":"75","author":"Nesvizhskii","year":"2003","journal-title":"Anal. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"D442","DOI":"10.1093\/nar\/gky1106","article-title":"The PRIDE database and related tools and resources in 2019: Improving support for quantification data","volume":"47","author":"Csordas","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.numecd.2019.08.001","article-title":"Insulin resistance, but not insulin response, during oral glucose tolerance test (OGTT) is associated to worse histological outcome in obese NAFLD","volume":"30","author":"Coccia","year":"2020","journal-title":"Nutr. Metab. Cardiovas."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.1007\/s00125-017-4340-1","article-title":"Use of HOMA-IR to diagnose non-alcoholic fatty liver disease: A population-based and inter-laboratory study","volume":"60","author":"Isokuortti","year":"2017","journal-title":"Diabetologia"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Fan, M., Choi, Y.J., Tang, Y., Bae, S.M., Yang, H.P., and Kim, E.K. (2019). Efficacy and Mechanism of Polymerized Anthocyanin from Grape-Skin Extract on High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease. Nutrients, 11.","DOI":"10.3390\/nu11112586"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Choi, Y.J., Lee, J.I., Fan, M., Tang, Y., Yoon, E.J., Ryu, Y.B., and Kim, E.K. (2020). Metabolomic Analysis of Morus Cultivar Root Extracts and Their Ameliorative Effect on Testosterone-Induced Prostate Enlargement in Sprague-Dawley Rats. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21041435"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/22\/8537\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:32:40Z","timestamp":1760178760000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/22\/8537"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,12]]},"references-count":46,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2020,11]]}},"alternative-id":["ijms21228537"],"URL":"https:\/\/doi.org\/10.3390\/ijms21228537","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2020,11,12]]}}}