{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T14:58:35Z","timestamp":1784818715529,"version":"3.55.0"},"reference-count":68,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T00:00:00Z","timestamp":1605657600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2018R1A2A3075600"],"award-info":[{"award-number":["NRF-2018R1A2A3075600"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF2017R1A5A2015391"],"award-info":[{"award-number":["NRF2017R1A5A2015391"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2015R1C1A2A01055438"],"award-info":[{"award-number":["NRF-2015R1C1A2A01055438"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In the present study, we examined the bone healing capacity of Meox2, a homeobox gene that plays essential roles in the differentiation of a range of developing tissues, and identified its putative function in palatogenesis. We applied the knocking down of Meox2 in human periodontal ligament fibroblasts to examine the osteogenic potential of Meox2. Additionally, we applied in vivo periodontitis induced experiment to reveal the possible application of Meox2 knockdown for 1 and 2 weeks in bone healing processes. We examined the detailed histomorphological changes using Masson\u2019s trichrome staining and micro-computed tomography evaluation. Moreover, we observed the localization patterns of various signaling molecules, including \u03b1-SMA, CK14, IL-1\u03b2, and MPO to examine the altered bone healing processes. Furthermore, we investigated the process of bone formation using immunohistochemistry of Osteocalcin and Runx2. On the basis of the results, we suggest that the knocking down of Meox2 via the activation of osteoblast and modulation of inflammation would be a plausible answer for bone regeneration as a gene therapy. Additionally, we propose that the purpose-dependent selection and application of developmental regulation genes are important for the functional regeneration of specific tissues and organs, where the pathological condition of tooth loss lesion would be.<\/jats:p>","DOI":"10.3390\/ijms21228701","type":"journal-article","created":{"date-parts":[[2020,11,18]],"date-time":"2020-11-18T07:41:00Z","timestamp":1605685260000},"page":"8701","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Facilitation of Bone Healing Processes Based on the Developmental Function of Meox2 in Tooth Loss Lesion"],"prefix":"10.3390","volume":"21","author":[{"given":"Tae-Young","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Kyung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Oral Biochemistry and Molecular Biology, Insititute of Translationnal Dental Sciences, Pusan National University School of Dentistry, Yangsan 50612, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yam","family":"Prasad Aryal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eui-Seon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2051-9865","authenticated-orcid":false,"given":"Sanjiv","family":"Neupane","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794-5215, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijin","family":"Sung","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elina","family":"Pokharel","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Yeol","family":"Yeon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Youn","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Dental Hygiene, College of Health Science, Gachon University, Incheon 21936, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Kwang","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine, School of Dentistry, IHBR, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Histology and Developmental Biology, Tokyo Dental College, Tokyo 101-0061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Hyeon","family":"An","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9049-6908","authenticated-orcid":false,"given":"Youngkyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wern-Joo","family":"Sohn","sequence":"additional","affiliation":[{"name":"Pre-Major of Cosmetics and Pharmaceutics, Daegu Haany University, Gyeongsan 38610, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Il-Ho","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Oral Biochemistry and Molecular Biology, Insititute of Translationnal Dental Sciences, Pusan National University School of Dentistry, Yangsan 50612, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seo-Young","family":"An","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6752-5683","authenticated-orcid":false,"given":"Jae-Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu 41940, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"dev184754","DOI":"10.1242\/dev.184754","article-title":"Molecular and cellular mechanisms of tooth development, homeostasis and 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