{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:56:37Z","timestamp":1787061397033,"version":"build-2736575974"},"reference-count":35,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2020,11,22]],"date-time":"2020-11-22T00:00:00Z","timestamp":1606003200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["NSC-100-2221-E-040-001-"],"award-info":[{"award-number":["NSC-100-2221-E-040-001-"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Diabetic foot ulcers (DFUs) caused by diabetes are prone to serious and persistent infections. If not treated properly, it will cause tissue necrosis or septicemia due to peripheral blood vessel embolism. Therefore, it is an urgent challenge to accelerate wound healing and reduce the risk of bacterial infection in patients. In clinical practice, DFUs mostly use hydrogel dressing to cover the surface of the affected area as an auxiliary treatment. Polyvinyl alcohol (PVA) is a hydrophilic hydrogel polymer widely used in dressings, drug delivery, and medical applications. However, due to its weak bioactivity and antibacterial ability, leads to limited application. Filler adding is a useful way to enhance the biocompatibility of PVA. In our study, cobalt-substituted hydroxyapatite (CoHA) powder was prepared by the electrochemically-deposited method. PVA and PVA-CoHA nanocomposite were prepared by the solvent casting method. The bioactivity of the PVA and composite was evaluated by immersed in simulated body fluid for 7 days. In addition, L929 cells and E. coli were used to evaluate the cytotoxicity and antibacterial tests of PVA and PVA-CoHA nanocomposite. The results show that the addition of CoHA increases the mechanical properties and biological activity of PVA. Biocompatibility evaluation showed no significant cytotoxicity of PVA-CoHA composite. In addition, a small amount of cobalt ion was released to the culture medium from the nanocomposite in the cell culture period and enhanced cell growth. The addition of CoHA also confirmed that it could inhibit the growth of E. coli. PVA-CoHA composite may have potential applications in diabetic trauma healing and wound dressing.<\/jats:p>","DOI":"10.3390\/ijms21228831","type":"journal-article","created":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T01:28:48Z","timestamp":1606094928000},"page":"8831","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Evaluation of Polyvinyl Alcohol\/Cobalt Substituted Hydroxyapatite Nanocomposite as a Potential Wound Dressing for Diabetic Foot Ulcers"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9441-6784","authenticated-orcid":false,"given":"Wei-Chun","family":"Lin","sequence":"first","affiliation":[{"name":"School of Dental Technology, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2103-6670","authenticated-orcid":false,"given":"Cheng-Ming","family":"Tang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Oral Sciences, Chung Shan Medical University, Taichung City 40201, Taiwan"},{"name":"Department of Dentistry, Chung Shan Medical University Hospital, Taichung City 40201, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1007\/BF00400697","article-title":"A multicentre study of the prevalence of diabetic peripheral neuropathy in the united kingdom hospital clinic population","volume":"36","author":"Young","year":"1993","journal-title":"Diabetologia"},{"key":"ref_2","unstructured":"Krasner, D., and Sibbald, R. (2001). Diabetic foot ulcer care: Assessment and management. Levin and O\u2019Neals The Diabetic Foot, Mosby. [6th ed.]."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"224","DOI":"10.12968\/jowc.1994.3.5.224","article-title":"Comparing two dressings in the treatment of diabetic foot ulcers","volume":"3","author":"Foster","year":"1994","journal-title":"J. Wound Care"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2892","DOI":"10.1002\/jps.21210","article-title":"Wound healing dressings and drug delivery systems: A review","volume":"97","author":"Boateng","year":"2008","journal-title":"J. Pharm. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.jaad.2007.08.048","article-title":"Treating the chronic wound: A practical approach to the care of nonhealing wounds and wound care dressings","volume":"58","author":"Fonder","year":"2008","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_6","first-page":"1","article-title":"Biodegradation study of some food packaging biopolymers based on pva","volume":"73","author":"Popa","year":"2016","journal-title":"Bull. Uasvm Anim. Sci. Biotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1002\/jbm.b.32694","article-title":"A review of polyvinyl alcohol and its uses in cartilage and orthopedic applications","volume":"100","author":"Baker","year":"2012","journal-title":"J. Biomed. Mater. Res. Part B Appl. Biomater."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2255","DOI":"10.1016\/S0142-9612(98)00128-8","article-title":"Cell viability and inflammatory response in hydrogel sponges implanted in the rabbit cornea","volume":"19","author":"Vijayasekaran","year":"1998","journal-title":"Biomaterials"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.jconrel.2016.10.032","article-title":"Ph-degradable pva-based nanogels via photo-crosslinking of thermo-preinduced nanoaggregates for controlled drug delivery","volume":"259","author":"Chen","year":"2017","journal-title":"J. Control. Release"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1016\/0014-3057(95)00061-5","article-title":"The effect of carbon black particle size on multifunctional additive-carbon black interaction","volume":"31","author":"Ismail","year":"1995","journal-title":"Eur. Polym. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"4895","DOI":"10.3390\/ma8084895","article-title":"Poly(vinyl alcohol) nanocomposites reinforced with bamboo charcoal nanoparticles: Mineralization behavior and characterization","volume":"8","author":"Tang","year":"2015","journal-title":"Materials"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1080\/10426910903032113","article-title":"Hydrothermal synthesis of nanosized pure and cobalt-exchanged hydroxyapatite","volume":"24","year":"2009","journal-title":"Mater. Manuf. Process."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1107\/S0021889809051395","article-title":"Crystal structure of cobalt-substituted calcium hydroxyapatite nanopowders prepared by hydrothermal processing","volume":"43","year":"2010","journal-title":"J. Appl. Crystallogr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.apsusc.2015.02.065","article-title":"Synthesis, structural characterisation and antibacterial activity of ag+-doped fluorapatite nanomaterials prepared by neutralization method","volume":"337","author":"Budimir","year":"2015","journal-title":"Appl. Surf. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1007\/s10853-012-6779-2","article-title":"Synthesis and characterization of iron-substituted hydroxyapatite via a simple ion-exchange procedure","volume":"48","author":"Kramer","year":"2013","journal-title":"J. Mater. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3580","DOI":"10.1016\/j.biomaterials.2010.01.083","article-title":"Enhancing in vivo vascularized bone formation by cobalt chloride-treated bone marrow stromal cells in a tissue engineered periosteum model","volume":"31","author":"Fan","year":"2010","journal-title":"Biomaterials"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.actbio.2016.07.045","article-title":"Hypoxia-mimicking co doped tio2 microporous coating on titanium with enhanced angiogenic and osteogenic activities","volume":"43","author":"Zhou","year":"2016","journal-title":"Acta Biomater."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"11323","DOI":"10.1016\/j.ceramint.2015.05.090","article-title":"Improving the osteogenic and angiogenic properties of synthetic hydroxyapatite by dual doping of bivalent cobalt and magnesium ion","volume":"41","author":"Kulanthaivel","year":"2015","journal-title":"Ceram. Int."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.actbio.2016.03.031","article-title":"Stimulatory effect of cobalt ions incorporated into calcium phosphate coatings on neovascularization in an in vivo intramuscular model in goats","volume":"36","author":"Fennema","year":"2016","journal-title":"Acta Biomater."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1016\/S1672-6529(14)60150-5","article-title":"Enhanced osteogenesis of nanosized cobalt-substituted hydroxyapatite","volume":"12","author":"Ignjatovic","year":"2015","journal-title":"J. Bionic Eng."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Lin, W.-C., Chuang, C.-C., Wang, P.-T., and Tang, C.-M. (2019). A comparative study on the direct and pulsed current electrodeposition of cobalt-substituted hydroxyapatite for magnetic resonance imaging application. Materials, 12.","DOI":"10.3390\/ma12010116"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1016\/j.dental.2019.02.023","article-title":"Long-term in vitro degradation behavior and biocompatibility of polycaprolactone\/cobalt-substituted hydroxyapatite composite for bone tissue engineering","volume":"35","author":"Lin","year":"2019","journal-title":"Dent. Mater."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1039\/C5TB02132D","article-title":"Gelatin\u2013poly(vinyl alcohol) porous biocomposites reinforced with graphene oxide as biomaterials","volume":"4","author":"Ionita","year":"2016","journal-title":"J. Mater. Chem. B"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1016\/j.carbpol.2015.09.113","article-title":"A novel gellan-pva nanofibrous scaffold for skin tissue regeneration: Fabrication and characterization","volume":"136","author":"Vashisth","year":"2016","journal-title":"Carbohydr. Polym."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.jcis.2010.04.091","article-title":"Synthesis, characterisation and antibacterial activity of pva\/teos\/ag-np hybrid thin films","volume":"349","author":"Bryaskova","year":"2010","journal-title":"J. Colloid Interface Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.msec.2016.04.042","article-title":"Swelling, mechanical and friction properties of pva\/pvp hydrogels after swelling in osmotic pressure solution","volume":"65","author":"Shi","year":"2016","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.carbpol.2014.12.027","article-title":"Mechanically stable antimicrobial chitosan-pva-silver nanocomposite coatings deposited on titanium implants","volume":"121","author":"Mishra","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1016\/j.physb.2010.01.095","article-title":"Chitosan filler effects on the experimental characterization, spectroscopic investigation and thermal studies of pva\/pvp blend films","volume":"405","author":"Abdelrazek","year":"2010","journal-title":"Phys. B Condens. Matter."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.cej.2011.06.015","article-title":"Synthesis and application of a highly efficient polyvinylcalix [4] arene tetraacetic acid resin for adsorptive removal of lead from aqueous solutions","volume":"172","author":"Adhikari","year":"2011","journal-title":"Chem. Eng. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.colsurfb.2012.04.046","article-title":"Physicochemical characterization and biocompatibility in vitro of biphasic calcium phosphate\/polyvinyl alcohol scaffolds prepared by freeze-drying method for bone tissue engineering applications","volume":"100","author":"Nie","year":"2012","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_31","first-page":"333","article-title":"Surface characterizations of variously treated titanium materials","volume":"16","author":"Lim","year":"2001","journal-title":"Int. J. Oral Maxillofac. Implant."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1177\/154405910408300704","article-title":"Enhanced bone apposition to a chemically modified sla titanium surface","volume":"83","author":"Buser","year":"2004","journal-title":"J. Dent. Res."},{"key":"ref_33","first-page":"24","article-title":"Synthesis of calcium hydroxyapatite powder from hen\u2019s eggshell","volume":"9","author":"Saeed","year":"2011","journal-title":"Iraqi J. Phys."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Lin, W.C., Chuang, C.C., Chang, C.J., Chiu, Y.H., and Tang, C.M. (2019). The effect of electrode topography on the magnetic properties and mri application of electrochemically-deposited, synthesized, cobalt-substituted hydroxyapatite. Nanomaterials, 9.","DOI":"10.3390\/nano9020200"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.jallcom.2017.01.306","article-title":"Thermal decomposition of metal complex precursor as route to the synthesis of co 3 o 4 nanoparticles: Antibacterial activity and mechanism","volume":"704","author":"Kavitha","year":"2017","journal-title":"J. Alloy. Compd."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/22\/8831\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:35:38Z","timestamp":1760178938000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/22\/8831"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,22]]},"references-count":35,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2020,11]]}},"alternative-id":["ijms21228831"],"URL":"https:\/\/doi.org\/10.3390\/ijms21228831","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,22]]}}}