{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T16:03:02Z","timestamp":1785513782862,"version":"3.56.0"},"reference-count":50,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T00:00:00Z","timestamp":1606176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Ted Mullin Fund","award":["x"],"award-info":[{"award-number":["x"]}]},{"name":"Comer Hospital RBC Race Funds","award":["x"],"award-info":[{"award-number":["x"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Intercellular junctions maintain the integrity of the endothelium. We previously found that the adherens and tight junctions between endothelial cells are disrupted by plasma extracellular vesicles from patients with sickle cell disease (especially those with Acute Chest Syndrome). In the current study, we evaluated the effects of these vesicles on endothelial gap junctions. The vesicles from sickle cell patients (isolated during episodes of Acute Chest Syndrome) disrupted gap junction structures earlier and more severely than the other classes of intercellular junctions (as detected by immunofluorescence). These vesicles were much more potent than those isolated at baseline from the same subject. The treatment of endothelial cells with these vesicles led to reduced levels of connexin43 mRNA and protein. These vesicles severely reduced intercellular communication (transfer of microinjected Neurobiotin). Our data suggest a hierarchy of progressive disruption of different intercellular connections between endothelial cells by circulating extracellular vesicles that may contribute to the pathophysiology of the endothelial disturbances in sickle cell disease.<\/jats:p>","DOI":"10.3390\/ijms21238884","type":"journal-article","created":{"date-parts":[[2020,11,24]],"date-time":"2020-11-24T02:34:42Z","timestamp":1606185282000},"page":"8884","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Gap Junctions between Endothelial Cells Are Disrupted by Circulating Extracellular Vesicles from Sickle Cell Patients with Acute Chest Syndrome"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8652-6414","authenticated-orcid":false,"given":"Joanna","family":"Gemel","sequence":"first","affiliation":[{"name":"Department of Pediatrics, University of Chicago, Chicago, IL 60637, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Chicago, Chicago, IL 60637, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabrielle","family":"Lapping-Carr","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Chicago, Chicago, IL 60637, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8044-1356","authenticated-orcid":false,"given":"Eric C.","family":"Beyer","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Chicago, Chicago, IL 60637, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1152\/physrev.00035.2003","article-title":"Endothelial cell-to-cell junctions: Molecular organization and role in vascular homeostasis","volume":"84","author":"Bazzoni","year":"2004","journal-title":"Physiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1016\/j.ceb.2010.07.006","article-title":"VE-cadherin: At the front, center, and sides of endothelial cell organization and function","volume":"22","author":"Harris","year":"2010","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1016\/j.bbamem.2007.09.003","article-title":"Endothelial adherens and tight junctions in vascular homeostasis, inflammation and angiogenesis","volume":"1778","author":"Wallez","year":"2008","journal-title":"Biochim. Biophys. Acta Biomembr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e12860","DOI":"10.1111\/apha.12860","article-title":"Mind the gap: Mechanisms regulating the endothelial barrier","volume":"222","author":"Radeva","year":"2018","journal-title":"Acta Physiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/bs.apha.2016.04.004","article-title":"Communication through gap junctions in the endothelium","volume":"77","author":"Schmidt","year":"2016","journal-title":"Adv. Pharmacol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1083\/jcb.92.1.183","article-title":"Intercellular junctions and transfer of small molecules in primary vascular endothelial cultures","volume":"92","author":"Larson","year":"1982","journal-title":"J. Cell Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1089\/ars.2008.2117","article-title":"Gap Junctions in the control of vascular function","volume":"11","author":"Figueroa","year":"2009","journal-title":"Antioxid. Redox Signal."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1160\/TH16-03-0210","article-title":"Connexins in endothelial barrier function\u2014Novel therapeutic targets countering vascular hyperpermeability","volume":"116","author":"Soon","year":"2016","journal-title":"Thromb. Haemost."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1146\/annurev-pathmechdis-012418-012838","article-title":"Pathophysiology of sickle cell disease","volume":"14","author":"Sundd","year":"2019","journal-title":"Annu. Rev. Pathol. Mech. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1160\/TH16-12-0943","article-title":"Microvesicles in vascular homeostasis and diseases. Position paper of the European Society of Cardiology (ESC) Working Group on Atherosclerosis and Vascular Biology","volume":"117","author":"Ridger","year":"2017","journal-title":"Thromb. Haemost."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.3389\/fphar.2019.01479","article-title":"The contrasting role of extracellular vesicles in vascular inflammation and tissue repair","volume":"10","author":"Oggero","year":"2019","journal-title":"Front. Pharmacol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/bjh.14112","article-title":"Microparticles in sickle cell anaemia: Promise and pitfalls","volume":"174","author":"Hebbel","year":"2016","journal-title":"Br. J. Haematol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.3389\/fphys.2020.01063","article-title":"Circulating Extracellular Vesicles and Endothelial Damage in Sickle Cell Disease","volume":"11","author":"Gemel","year":"2020","journal-title":"Front. Physiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1111\/bjh.14104","article-title":"Extracellular microvesicle microRNAs in children with sickle cell anaemia with divergent clinical phenotypes","volume":"174","author":"Khalyfa","year":"2016","journal-title":"Br. J. Haematol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1002\/ppul.23698","article-title":"Exosomes contribute to endothelial integrity and acute chest syndrome risk: Preliminary findings","volume":"52","author":"Khalyfa","year":"2017","journal-title":"Pediatr. Pulmonol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Lapping-Carr, G., Gemel, J., Mao, Y., Sparks, G., Harrington, M., Peddinti, R., and Beyer, E.C. (2020). Circulating extracellular vesicles from patients with acute chest syndrome disrupt adherens junctions between endothelial cells. Pediatr. Res.","DOI":"10.1038\/s41390-020-0923-5"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.biocel.2011.10.005","article-title":"Exosomes: Small vesicles participating in intercellular communication","volume":"44","author":"Ludwig","year":"2012","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_18","first-page":"354","article-title":"Alternative methods for characterization of extracellular vesicles","volume":"3","author":"Balaj","year":"2012","journal-title":"Front. Physiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/0955-0674(90)90086-T","article-title":"Gap junctional intercellular communication and the regulation of connexin expression and function","volume":"2","author":"Musil","year":"1990","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1161\/01.RES.83.6.629","article-title":"Rapid turnover of Connexin43 in the adult rat heart","volume":"83","author":"Beardslee","year":"1998","journal-title":"Circ. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"10761","DOI":"10.1074\/jbc.M109.053348","article-title":"E-cadherin differentially regulates the assembly of Connexin43 and Connexin32 into gap junctions in human squamous carcinoma cells","volume":"285","author":"Chakraborty","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4089","DOI":"10.1091\/mbc.e10-05-0403","article-title":"Assembly of Connexin43 into gap junctions is regulated differentially by E-Cadherin and N-Cadherin in rat liver epithelial cells","volume":"21","author":"Govindarajan","year":"2010","journal-title":"Mol. Biol. Cell"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1083\/jcb.200105047","article-title":"Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions","volume":"154","author":"Xu","year":"2001","journal-title":"J. Cell Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1161\/01.RES.0000181132.11393.18","article-title":"Cardiac-specific loss of N-Cadherin leads to alteration in connexins with conduction sSlowing and arrhythmogenesis","volume":"97","author":"Li","year":"2005","journal-title":"Circ. Res."},{"key":"ref_25","first-page":"243","article-title":"Formation of the gap junction nexus: Binding partners for connexins","volume":"96","author":"Duffy","year":"2002","journal-title":"J. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.bbamem.2017.05.008","article-title":"The connexin 43 C-terminus: A tail of many tales","volume":"1860","author":"Leithe","year":"2018","journal-title":"Biochim. Biophys. Acta Biomembr."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3846","DOI":"10.1038\/s41467-018-06368-x","article-title":"Gap junction protein Connexin-43 is a direct transcriptional regulator of N-cadherin in vivo","volume":"9","author":"Kotini","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2615","DOI":"10.1096\/fj.201700699R","article-title":"Connexin 43 gap junctions contribute to brain endothelial barrier hyperpermeability in familial cerebral cavernous malformations type III by modulating tight junction structure","volume":"32","author":"Johnson","year":"2018","journal-title":"FASEB J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2911","DOI":"10.1182\/blood-2012-12-473017","article-title":"Regulation of vascular leak and recovery from ischemic injury by general and VE-cadherin-restricted miRNA antagonists of miR-27","volume":"122","author":"Young","year":"2013","journal-title":"Blood"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1002\/1873-3468.12565","article-title":"MicroRNA-22 regulates inflammation and angiogenesis via targeting VE-cadherin","volume":"591","author":"Gu","year":"2017","journal-title":"FEBS Lett."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1038\/onc.2012.68","article-title":"microRNA-125b inhibits tube formation of blood vessels through translational suppression of VE-cadherin","volume":"32","author":"Muramatsu","year":"2013","journal-title":"Oncogene"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e009244","DOI":"10.1161\/JAHA.118.009244","article-title":"Inhibition of MicroRNA-155 supports endothelial tight junction integrity following oxygen-glucose deprivation","volume":"7","author":"Gardiner","year":"2018","journal-title":"J. Am. Heart Assoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e944446","DOI":"10.4161\/21688362.2014.944446","article-title":"MicroRNAs regulate tight junction proteins and modulate epithelial\/endothelial barrier functions","volume":"2","author":"Cichon","year":"2014","journal-title":"Tissue Barriers"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2016\/5078627","article-title":"MicroRNA Regulation of endothelial junction proteins and clinical consequence","volume":"2016","author":"Zhuang","year":"2016","journal-title":"Mediat. Inflamm."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.yjmcc.2014.04.024","article-title":"Downregulation of connexin43 by microRNA-130a in cardiomyocytes results in cardiac arrhythmias","volume":"74","author":"Osbourne","year":"2014","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.1016\/j.hlc.2018.09.008","article-title":"MicroRNA-206 downregulates Connexin43 in cardiomyocytes to induce cardiac arrhythmias in a transgenic mouse model","volume":"28","author":"Jin","year":"2019","journal-title":"Hear. Lung Circ."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1038\/nm1569","article-title":"The muscle-specific microRNA miR-1 regulates cardiac arrhythmogenic potential by targeting GJA1 and KCNJ2","volume":"13","author":"Yang","year":"2007","journal-title":"Nat. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"H838","DOI":"10.1152\/ajpheart.1989.256.3.H838","article-title":"Conduction of vasomotor responses in arterioles: A role for cell-to-cell coupling?","volume":"256","author":"Segal","year":"1989","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1124\/pr.115.012062","article-title":"Connexins in cardiovascular and neurovascular health and disease: Pharmacological Implications","volume":"69","author":"Leybaert","year":"2017","journal-title":"Pharmacol. Rev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1942","DOI":"10.1038\/jcbfm.2011.86","article-title":"Connexin channels provide a target to manipulate brain endothelial calcium dynamics and blood\u2014brain barrier permeability","volume":"31","author":"Culot","year":"2011","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1002\/jcp.24563","article-title":"Functional role of connexins and pannexins in the interaction between vascular and nervous system","volume":"229","author":"Gaete","year":"2014","journal-title":"J. Cell. Physiol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Okamoto, T., and Suzuki, K. (2017). The role of gap junction-mediated endothelial cell-cell interaction in the crosstalk between inflammation and blood coagulation. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18112254"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1038\/s41419-020-2392-5","article-title":"Cx43 channels and signaling via IP3\/Ca2+, ATP, and ROS\/NO propagate radiation-induced DNA damage to non-irradiated brain microvascular endothelial cells","volume":"11","author":"Hoorelbeke","year":"2020","journal-title":"Cell Death Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"104600","DOI":"10.1016\/j.neuint.2019.104600","article-title":"Polarized hemichannel opening of pannexin 1\/connexin 43 contributes to dysregulation of transport function in blood-brain barrier endothelial cells","volume":"132","author":"Tachikawa","year":"2020","journal-title":"Neurochem. Int."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.1161\/01.RES.66.4.1074","article-title":"Gap junction messenger RNA expression by vascular wall cells","volume":"66","author":"Larson","year":"1990","journal-title":"Circ. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"H729","DOI":"10.1152\/ajpheart.1995.268.2.H729","article-title":"Connexin 43 and connexin 40 gap junctional proteins are present in arteriolar smooth muscle and endothelium in vivo","volume":"268","author":"Little","year":"1995","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1161\/01.RES.83.6.636","article-title":"Connexin43 is highly localized to sites of disturbed flow in rat aortic endothelium but connexin37 and connexin40 are more uniformly distributed","volume":"83","author":"Gabriels","year":"1998","journal-title":"Circ. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.yjmcc.2014.08.021","article-title":"Connexin40 abnormalities and atrial fibrillation in the human heart","volume":"76","author":"Gemel","year":"2014","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.yjmcc.2009.05.014","article-title":"Connexin40 and connexin43 determine gating properties of atrial gap junction channels","volume":"48","author":"Lin","year":"2010","journal-title":"J. Mol. Cell. Cardiol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/23\/8884\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:36:23Z","timestamp":1760178983000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/23\/8884"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,24]]},"references-count":50,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["ijms21238884"],"URL":"https:\/\/doi.org\/10.3390\/ijms21238884","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,24]]}}}