{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:33:47Z","timestamp":1791192827693,"version":"4.1.0"},"reference-count":196,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2020,11,26]],"date-time":"2020-11-26T00:00:00Z","timestamp":1606348800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP20K18501"],"award-info":[{"award-number":["JP20K18501"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Periodontal disease is a chronic inflammatory disease caused by periodontal bacteria. Recently, periodontal phototherapy, treatment using various types of lasers, has attracted attention. Photobiomodulation, the biological effect of low-power laser irradiation, has been widely studied. Although many types of lasers are applied in periodontal phototherapy, molecular biological effects of laser irradiation on cells in periodontal tissues are unclear. Here, we have summarized the molecular biological effects of diode, Nd:YAG, Er:YAG, Er,Cr:YSGG, and CO2 lasers irradiation on cells in periodontal tissues. Photobiomodulation by laser irradiation enhanced cell proliferation and calcification in osteoblasts with altering gene expression. Positive effects were observed in fibroblasts on the proliferation, migration, and secretion of chemokines\/cytokines. Laser irradiation suppressed gene expression related to inflammation in osteoblasts, fibroblasts, human periodontal ligament cells (hPDLCs), and endothelial cells. Furthermore, recent studies have revealed that laser irradiation affects cell differentiation in hPDLCs and stem cells. Additionally, some studies have also investigated the effects of laser irradiation on endothelial cells, cementoblasts, epithelial cells, osteoclasts, and osteocytes. The appropriate irradiation power was different for each laser apparatus and targeted cells. Thus, through this review, we tried to shed light on basic research that would ultimately lead to clinical application of periodontal phototherapy in the future.<\/jats:p>","DOI":"10.3390\/ijms21239002","type":"journal-article","created":{"date-parts":[[2020,11,26]],"date-time":"2020-11-26T22:00:33Z","timestamp":1606428033000},"page":"9002","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["In Vitro Cytological Responses against Laser Photobiomodulation for Periodontal Regeneration"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2843-173X","authenticated-orcid":false,"given":"Yujin","family":"Ohsugi","sequence":"first","affiliation":[{"name":"Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Niimi","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuyoshi","family":"Shimohira","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Hatasa","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayaka","family":"Katagiri","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Aoki","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5461-7298","authenticated-orcid":false,"given":"Takanori","family":"Iwata","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,26]]},"reference":[{"key":"ref_1","unstructured":"Lang, N.P., and Lindhe, J. (2015). Clinical Periodontology and Implant Dentistry, John Wiley & Sons."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"17038","DOI":"10.1038\/nrdp.2017.38","article-title":"Periodontal diseases","volume":"3","author":"Kinane","year":"2017","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/jcpe.13245","article-title":"Subgingival instrumentation for treatment of periodontitis. A systematic review","volume":"47","author":"Suvan","year":"2020","journal-title":"J. Clin. Periodontol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1111\/prd.12080","article-title":"Periodontal and peri-implant wound healing following laser therapy","volume":"68","author":"Aoki","year":"2015","journal-title":"Periodontoloy 2000"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1111\/prd.12123","article-title":"Lasers in minimally invasive periodontal and peri-implant therapy","volume":"71","author":"Mizutani","year":"2016","journal-title":"Periodontoloy 2000"},{"key":"ref_6","first-page":"739","article-title":"Photobiomodulation in Periodontology and Implant Dentistry: Part 1","volume":"37","author":"Gholami","year":"2019","journal-title":"Photobiomodul. Photomed. Laser Surg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s10103-017-2384-6","article-title":"Laser photobiomodulation in pressure ulcer healing of human diabetic patients: Gene expression analysis of inflammatory biochemical markers","volume":"33","author":"Ruh","year":"2018","journal-title":"Lasers Med. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1089\/pho.2016.4195","article-title":"Comparative Study of the Physiotherapeutic and Drug Protocol and Low-Level Laser Irradiation in the Treatment of Pain Associated with Temporomandibular Dysfunction","volume":"34","author":"Cavalcanti","year":"2016","journal-title":"Photomed. Laser Surg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1039\/c8pp00176f","article-title":"Photobiomodulation: Lasers vs. light emitting diodes?","volume":"17","author":"Heiskanen","year":"2018","journal-title":"Photochem. Photobiol. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1089\/pho.2015.9848","article-title":"Low-level light\/laser therapy versus photobiomodulation therapy","volume":"33","author":"Anders","year":"2015","journal-title":"Photomed. Laser Surg."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1364\/AO.12.000555","article-title":"Optical constants of water in the 200-nm to 200-\u00b5m wavelength region","volume":"12","author":"Hale","year":"1973","journal-title":"Appl. Opt."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Niemz, M.H. (1996). Laser-Tissue Interaction. Fundamentals and Applications, Springer.","DOI":"10.1007\/978-3-662-03193-3"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/j.1600-0757.2004.03679.x","article-title":"Lasers in non-surgical periodontal therapy","volume":"36","author":"Aoki","year":"2004","journal-title":"Periodontology 2000"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1196\/annals.1365.035","article-title":"Bone remodeling","volume":"1092","author":"Hadjidakis","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/PL00011357","article-title":"Stimulation of MCM3 gene expression in osteoblast by low level laser irradiation","volume":"16","author":"Yamamoto","year":"2001","journal-title":"Lasers Med. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1007\/s10103-003-0255-9","article-title":"Effect of low-level laser irradiation on osteoglycin gene expression in osteoblasts","volume":"18","author":"Hamajima","year":"2003","journal-title":"Lasers Med. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1089\/pho.2007.2055","article-title":"The effects of laser irradiation on osteoblast and osteosarcoma cell proliferation and differentiation in vitro","volume":"25","author":"Renno","year":"2007","journal-title":"Photomed. Laser Surg."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1002\/lsm.20880","article-title":"Low-intensity laser irradiation stimulates mineralization via increased BMPs in MC3T3-E1 cells","volume":"42","author":"Fujimoto","year":"2010","journal-title":"Lasers Surg. Med."},{"key":"ref_19","first-page":"47","article-title":"Enhancement of microtubule-associated protein-1 Alpha gene expression in osteoblasts by low level laser irradiation","volume":"20","author":"Kanenari","year":"2011","journal-title":"Laser"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1007\/s10103-014-1556-x","article-title":"Laser-induced osteoblast proliferation is mediated by ROS production","volume":"29","author":"Migliario","year":"2014","journal-title":"Lasers Med. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/s10103-012-1238-5","article-title":"Laser and light-emitting diode effects on pre-osteoblast growth and differentiation","volume":"29","author":"Pagin","year":"2014","journal-title":"Lasers Med. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.jphotobiol.2017.02.020","article-title":"Low level laser therapy modulates viability, alkaline phosphatase and matrix metalloproteinase-2 activities of osteoblasts","volume":"169","author":"Oliveira","year":"2017","journal-title":"J. Photochem. Photobiol. B"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1007\/s10103-017-2145-6","article-title":"A novel combination treatment to stimulate bone healing and regeneration under hypoxic conditions: Photobiomodulation and melatonin","volume":"32","author":"Son","year":"2017","journal-title":"Lasers Med. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1007\/s10103-017-2269-8","article-title":"Laser irradiation promotes the proliferation of mouse pre-osteoblast cell line MC3T3-E1 through hedgehog signaling pathway","volume":"32","author":"Li","year":"2017","journal-title":"Lasers Med. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1007\/s10103-017-2426-0","article-title":"Effects of high-frequency near-infrared diode laser irradiation on the proliferation and migration of mouse calvarial osteoblasts","volume":"33","author":"Kunimatsu","year":"2018","journal-title":"Lasers Med. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1002\/lsm.22775","article-title":"Low-level ultrahigh-frequency and ultrashort-pulse blue laser irradiation enhances osteoblast extracellular calcification by upregulating proliferation and differentiation via transient receptor potential vanilloid 1","volume":"50","author":"Mikami","year":"2018","journal-title":"Lasers Surg. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1034\/j.1600-0544.2001.040102.x","article-title":"The effects of low level laser irradiation on osteoblastic cells","volume":"4","author":"Coombe","year":"2001","journal-title":"Clin. Orthod. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.ijom.2012.03.026","article-title":"Effect of low level laser therapy and zoledronate on the viability and ALP activity of Saos-2 cells","volume":"42","author":"Bayram","year":"2013","journal-title":"Int. J. Oral. Maxillofac. Surg."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"128006","DOI":"10.1117\/1.JBO.18.12.128006","article-title":"Investigation of low-level laser therapy potentiality on proliferation and differentiation of human osteoblast-like cells in the absence\/presence of osteogenic factors","volume":"18","author":"Bloise","year":"2013","journal-title":"J. Biomed. Opt."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"108002","DOI":"10.1117\/1.JBO.19.10.108002","article-title":"Different doses of low-level laser irradiation modulate the in vitro response of osteoblast-like cells","volume":"19","author":"Checchi","year":"2014","journal-title":"J. Biomed. Opt."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"78002","DOI":"10.1117\/1.JBO.20.7.078002","article-title":"Photobiomodulation with low-level diode laser promotes osteoblast migration in an in vitro micro wound model","volume":"20","author":"Tschon","year":"2015","journal-title":"J. Biomed. Opt."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1111\/jop.12442","article-title":"The enhancing effect of a laser photochemotherapy with cisplatin or zolendronic acid in primary human osteoblasts and osteosarcoma cells in vitro","volume":"45","author":"Heymann","year":"2016","journal-title":"J. Oral. Pathol. Med."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Tani, A., Chellini, F., Giannelli, M., Nosi, D., Zecchi-Orlandini, S., and Sassoli, C. (2018). Red (635 nm), Near-Infrared (808 nm) and Violet-Blue (405 nm) Photobiomodulation Potentiality on Human Osteoblasts and Mesenchymal Stromal Cells: A Morphological and Molecular in vitro Study. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19071946"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1007\/s00508-008-0932-6","article-title":"Initial effects of low-level laser therapy on growth and differentiation of human osteoblast-like cells","volume":"120","author":"Stein","year":"2008","journal-title":"Wien Klin Wochenschr"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1002\/lsm.20762","article-title":"Superpulsed laser irradiation increases osteoblast activity via modulation of bone morphogenetic factors","volume":"41","author":"Saracino","year":"2009","journal-title":"Lasers Surg. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1007\/s10103-011-1006-y","article-title":"Low-level diode laser therapy reduces lipopolysaccharide (LPS)-induced bone cell inflammation","volume":"27","author":"Huang","year":"2012","journal-title":"Lasers Med. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1177\/1099800413482695","article-title":"Effect and clinical implications of the low-energy diode laser on bone cell proliferation","volume":"16","author":"Huertas","year":"2014","journal-title":"Biol. Res. Nurs."},{"key":"ref_38","first-page":"255","article-title":"Effect of low-level laser irradiation on osteoblast-like cells cultured on porous hydroxyapatite scaffolds","volume":"49","author":"Panseri","year":"2013","journal-title":"Ann. Ist. Super Sanita"},{"key":"ref_39","first-page":"1479","article-title":"The effects of low-level diode laser irradiation on differentiation, antigenic profile, and phagocytic capacity of osteoblast-like cells (MG-63)","volume":"29","author":"Ruiz","year":"2014","journal-title":"Lasers Med. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1016\/j.jcms.2015.08.026","article-title":"The effect of low-level diode laser therapy on early differentiation of osteoblast via BMP-2\/TGF-beta1 and its receptors","volume":"43","author":"Ruiz","year":"2015","journal-title":"J. Craniomaxillofac. Surg."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1007\/s10103-012-1109-0","article-title":"Low-level laser therapy induces the expressions of BMP-2, osteocalcin, and TGF-beta1 in hypoxic-cultured human osteoblasts","volume":"28","author":"Pyo","year":"2013","journal-title":"Lasers Med. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"128001","DOI":"10.1117\/1.JBO.18.12.128001","article-title":"Effect of 940 nm low-level laser therapy on osteogenesis in vitro","volume":"18","author":"Jawad","year":"2013","journal-title":"J. Biomed. Opt."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"14","DOI":"10.3892\/br.2014.389","article-title":"Effects of a low-level diode laser on oral keratinocytes, oral fibroblasts, endothelial cells and osteoblasts incubated with bisphosphonates: An in vitro study","volume":"3","author":"Walter","year":"2015","journal-title":"Biomed. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s40902-016-0095-8","article-title":"Effect of low-level laser therapy on bisphosphonate-treated osteoblasts","volume":"38","author":"Shin","year":"2016","journal-title":"Maxillofac. Plast Reconstr. Surg."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1007\/s10103-017-2153-6","article-title":"Investigation of photobiomodulation potentiality by 635 and 809 nm lasers on human osteoblasts","volume":"32","author":"Gulsoy","year":"2017","journal-title":"Lasers Med. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1007\/s10103-018-2530-9","article-title":"Indocyanine green-mediated photobiomodulation on human osteoblast cells","volume":"33","author":"Ates","year":"2018","journal-title":"Lasers Med. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"259","DOI":"10.5125\/jkaoms.2018.44.6.259","article-title":"Combined effect of recombinant human bone morphogenetic protein-2 and low level laser irradiation on bisphosphonate-treated osteoblasts","volume":"44","author":"Jeong","year":"2018","journal-title":"J. Korean Assoc. Oral. Maxillofac. Surg."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1002\/lsm.20298","article-title":"Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence","volume":"38","author":"Fujihara","year":"2006","journal-title":"Lasers Surg. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1007\/s00223-006-0072-9","article-title":"Optimal low-energy laser irradiation causes temporal G2\/M arrest on rat calvarial osteoblasts","volume":"79","author":"Fukuhara","year":"2006","journal-title":"Calcif. Tissue Int."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1002\/lsm.20521","article-title":"Low-intensity laser irradiation stimulates bone nodule formation via insulin-like growth factor-I expression in rat calvarial cells","volume":"39","author":"Shimizu","year":"2007","journal-title":"Lasers Surg. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1089\/pho.2008.2283","article-title":"Low-intensity pulsed laser irradiation affects RANKL and OPG mRNA expression in rat calvarial cells","volume":"27","author":"Xu","year":"2009","journal-title":"Photomed. Laser Surg."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/S8756-3282(97)00294-9","article-title":"Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells","volume":"22","author":"Ozawa","year":"1998","journal-title":"Bone"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"55","DOI":"10.2334\/josnusd.43.55","article-title":"Pulse irradiation of low-power laser stimulates bone nodule formation","volume":"43","author":"Ueda","year":"2001","journal-title":"J. Oral. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1089\/104454703322564479","article-title":"Effects of pulse frequency of low-level laser therapy (LLLT) on bone nodule formation in rat calvarial cells","volume":"21","author":"Ueda","year":"2003","journal-title":"J. Clin. Laser Med. Surg."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3503","DOI":"10.1016\/j.biomaterials.2004.09.033","article-title":"Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material","volume":"26","author":"Khadra","year":"2005","journal-title":"Biomaterials"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Cardoso, M.V., do Vale Placa, R., Sant\u2019Ana, A.C.P., Greghi, S.L.A., Zangrando, M.S.R., de Rezende, M.L.R., Oliveira, R.C., and Damante, C.A. (2020). Laser and LED photobiomodulation effects in osteogenic or regular medium on rat calvaria osteoblasts obtained by newly forming bone technique. Lasers Med. Sci.","DOI":"10.1007\/s10103-020-03056-5"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1590\/S0103-64402010000600003","article-title":"Effects of low-level laser therapy on human osteoblastic cells grown on titanium","volume":"21","author":"Petri","year":"2010","journal-title":"Braz. Dent J."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1007\/s10103-012-1165-5","article-title":"Low-level laser therapy vs. pulsed electromagnetic field on neonatal rat calvarial osteoblast-like cells","volume":"28","author":"Emes","year":"2013","journal-title":"Lasers Med. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e201900210","DOI":"10.1590\/s0102-8650201900210","article-title":"Effect of low power laser in biomodulation of cultured osteoblastic cells of Wistar rats1","volume":"34","author":"Morsoleto","year":"2019","journal-title":"Acta Cir. Bras"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1089\/pho.2007.2101","article-title":"Evaluation of low-level laser therapy of osteoblastic cells","volume":"26","author":"Zangaro","year":"2008","journal-title":"Photomed Laser Surg."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1034\/j.1600-0501.2000.011006540.x","article-title":"Biostimulation of bone marrow cells with a diode soft laser","volume":"11","author":"Dortbudak","year":"2000","journal-title":"Clin. Oral. Implant. Res."},{"key":"ref_62","first-page":"603","article-title":"Effect of low-level laser irradiation on osteoblast proliferation and bone formation","volume":"25","author":"Grassi","year":"2011","journal-title":"J. Biol. Regul. Homeost. Agents"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1007\/s10103-018-2453-5","article-title":"Effects of 915 nm laser irradiation on human osteoblasts: A preliminary in vitro study","volume":"33","author":"Mergoni","year":"2018","journal-title":"Lasers Med. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1002\/jcb.22872","article-title":"Low-level laser irradiation enhances BMP-induced osteoblast differentiation by stimulating the BMP\/Smad signaling pathway","volume":"111","author":"Hirata","year":"2010","journal-title":"J. Cell Biochem."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1177\/40.8.1619274","article-title":"In situ hybridization of bone matrix proteins in undecalcified adult rat bone sections","volume":"40","author":"Ikeda","year":"1992","journal-title":"J. Histochem. Cytochem."},{"key":"ref_66","first-page":"168","article-title":"Molecular Mechanism of Runx2-Dependent Bone Development","volume":"43","author":"Komori","year":"2020","journal-title":"Mol. Cells"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0092-8674(01)00622-5","article-title":"The Novel Zinc Finger-Containing Transcription Factor Osterix Is Required for Osteoblast Differentiation and Bone Formation","volume":"108","author":"Nakashima","year":"2002","journal-title":"Cell"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1093\/jb\/mvp148","article-title":"Bone morphogenetic protein receptors and signal transduction","volume":"147","author":"Miyazono","year":"2010","journal-title":"J. Biochem."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/37284","article-title":"TGF-beta signalling from cell membrane to nucleus through SMAD proteins","volume":"390","author":"Heldin","year":"1997","journal-title":"Nature"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1359\/jbmr.060315","article-title":"Function and regulation of osteopontin in response to mechanical stress","volume":"21","author":"Fujihara","year":"2006","journal-title":"J. Bone Min. Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1002\/jcp.1080","article-title":"Divergence and convergence of TGF-beta\/BMP signaling","volume":"187","author":"Miyazono","year":"2001","journal-title":"J. Cell Physiol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1210\/mend.11.11.0011","article-title":"Stage-specific expression of Dlx5 during osteoblast differentiation: Involvement in regulation of osteocalcin gene expression","volume":"11","author":"Ryoo","year":"1997","journal-title":"Mol. Endocrinol."},{"key":"ref_73","first-page":"2651","article-title":"The diverse functions of osteoglycin: A deceitful dwarf, or a master regulator of disease?","volume":"30","author":"Deckx","year":"2016","journal-title":"Faseb J. Off. Publ. Fed. Am. Soc. Exp. Biol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1007\/s11914-019-00523-z","article-title":"Osteoglycin and Bone-a Systematic Review","volume":"17","author":"Viggers","year":"2019","journal-title":"Curr. Osteoporos. Rep."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/s10103-006-0398-6","article-title":"Effects of Nd:Yag laser irradiation on osteoblast cell cultures","volume":"21","author":"Arisu","year":"2006","journal-title":"Lasers Med. Sci."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1002\/lsm.20861","article-title":"Low pulse energy Nd:YAG laser irradiation exerts a biostimulative effect on different cells of the oral microenvironment: \u201can in vitro study\u201d","volume":"42","author":"Chellini","year":"2010","journal-title":"Lasers Surg. Med."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"e12417","DOI":"10.1111\/cpr.12417","article-title":"Low level laser therapy induces increased viability and proliferation in isolated cancer cells","volume":"51","author":"Kara","year":"2018","journal-title":"Cell Prolif."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/s10103-018-2586-6","article-title":"Effects of Nd:YAG low-level laser irradiation on cultured human osteoblasts migration and ATP production: In vitro study","volume":"34","author":"Tsuka","year":"2019","journal-title":"Lasers Med. Sci."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"138","DOI":"10.34172\/jlms.2020.24","article-title":"Examination of the Effect of the Combined Use of Nd: YAG Laser Irradiation and Mechanical Force Loading on Bone Metabolism Using Cultured Human Osteoblasts","volume":"11","author":"Tsuka","year":"2020","journal-title":"J. Lasers Med. Sci."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1002\/lsm.20870","article-title":"High power-pulsed Nd:YAG laser as a new stimulus to induce BMP-2 expression in MC3T3-E1 osteoblasts","volume":"42","author":"Kim","year":"2010","journal-title":"Lasers Surg. Med."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/8756-3282(94)00040-9","article-title":"Osteoblasts and osteoclasts in adult human osteophyte tissue express the mRNAs for insulin-like growth factors I and II and the type 1 IGF receptor","volume":"16","author":"Middleton","year":"1995","journal-title":"Bone"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/s10103-004-0305-y","article-title":"Effects of an Er:YAG laser on mitochondrial activity of human osteosarcoma-derived osteoblasts in vitro","volume":"19","author":"Schwarz","year":"2004","journal-title":"Lasers Med. Sci."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1007\/s10103-010-0761-5","article-title":"Low-level Er:YAG laser irradiation enhances osteoblast proliferation through activation of MAPK\/ERK","volume":"25","author":"Aleksic","year":"2010","journal-title":"Lasers Med. Sci."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"459","DOI":"10.3389\/fcell.2020.00459","article-title":"Effects of Low-Level Er:YAG Laser Irradiation on Proliferation and Calcification of Primary Osteoblast-Like Cells Isolated From Rat Calvaria","volume":"8","author":"Niimi","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1128\/MMBR.00031-10","article-title":"Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases","volume":"75","author":"Cargnello","year":"2011","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1016\/j.bone.2011.06.033","article-title":"Differential roles of CCN family proteins during osteoblast differentiation: Involvement of Smad and MAPK signaling pathways","volume":"49","author":"Kawaki","year":"2011","journal-title":"Bone"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1002\/jcb.23287","article-title":"Integration of BMP, Wnt, and notch signaling pathways in osteoblast differentiation","volume":"112","author":"Lin","year":"2011","journal-title":"J. Cell Biochem."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"51","DOI":"10.2334\/josnusd.53.51","article-title":"Transcriptional regulation of bone sialoprotein gene by CO(2) laser irradiation","volume":"53","author":"Sasaki","year":"2011","journal-title":"J. Oral. Sci."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1038\/s41556-018-0237-6","article-title":"Stem cell dynamics, migration and plasticity during wound healing","volume":"21","author":"Dekoninck","year":"2019","journal-title":"Nat. Cell Biol."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"407","DOI":"10.12968\/jowc.2013.22.8.407","article-title":"Wound healing and the role of fibroblasts","volume":"22","author":"Bainbridge","year":"2013","journal-title":"J. Wound Care"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1083\/jcb.124.4.401","article-title":"Fibroblasts, myofibroblasts, and wound contraction","volume":"124","author":"Grinnell","year":"1994","journal-title":"J. Cell Biol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"337","DOI":"10.3934\/biophy.2017.3.337","article-title":"Mechanisms and applications of the anti-inflammatory effects of photobiomodulation","volume":"4","author":"Hamblin","year":"2017","journal-title":"Aims Biophys."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1007\/s10103-016-2013-9","article-title":"Laser biostimulation of wound healing: Bioimpedance measurements support histology","volume":"31","author":"Solmaz","year":"2016","journal-title":"Lasers Med. Sci."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1034\/j.1600-0722.2000.00783.x","article-title":"Inhibitory effect of low-level laser irradiation on LPS-stimulated prostaglandin E2 production and cyclooxygenase-2 in human gingival fibroblasts","volume":"108","author":"Sakurai","year":"2000","journal-title":"Eur. J. Oral. Sci."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/s101030050045","article-title":"Reduction of Plasminogen Activator Activity Stimulated by Lipopolysaccharide from Periodontal Pathogen in Human Gingival Fibroblasts by Low-energy Laser Irradiation","volume":"15","author":"Takema","year":"2000","journal-title":"Lasers Med. Sci."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1007\/PL00011358","article-title":"Inhibition of interleukin-1beta production and gene expression in human gingival fibroblasts by low-energy laser irradiation","volume":"16","author":"Nomura","year":"2001","journal-title":"Lasers Med. Sci."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1002\/lsm.1107","article-title":"Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence","volume":"29","author":"Rigau","year":"2001","journal-title":"Lasers Surg. Med."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"719452","DOI":"10.1155\/2012\/719452","article-title":"In vitro wound healing improvement by low-level laser therapy application in cultured gingival fibroblasts","volume":"2012","author":"Basso","year":"2012","journal-title":"Int. J. Dent"},{"key":"ref_99","first-page":"1071","article-title":"The Effects of Low Level Laser Therapy on the Expression of Collagen Type I Gene and Proliferation of Human Gingival Fibroblasts (Hgf3-Pi 53): In vitro Study","volume":"16","author":"Frozanfar","year":"2013","journal-title":"Iran J. Basic Med. Sci."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s10103-007-0477-3","article-title":"Effects of laser irradiation on the release of basic fibroblast growth factor (bFGF), insulin like growth factor-1 (IGF-1), and receptor of IGF-1 (IGFBP3) from gingival fibroblasts","volume":"23","author":"Saygun","year":"2008","journal-title":"Lasers Med. Sci."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1111\/php.12445","article-title":"Biomodulation of Inflammatory Cytokines Related to Oral Mucositis by Low-Level Laser Therapy","volume":"91","author":"Basso","year":"2015","journal-title":"Photochem. Photobiol."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1007\/s10103-010-0879-5","article-title":"Effects of different setting of diode laser on the mRNA expression of growth factors and type I collagen of human gingival fibroblasts","volume":"27","author":"Hakki","year":"2012","journal-title":"Lasers Med. Sci."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1007\/s10103-008-0582-y","article-title":"Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts","volume":"24","author":"Damante","year":"2009","journal-title":"Lasers Med. Sci."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1002\/lsm.1072","article-title":"Effect of diode laser irradiation on the survival rate of gingival fibroblast cell cultures","volume":"28","author":"Kreisler","year":"2001","journal-title":"Lasers Surg. Med."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1002\/lsm.10107","article-title":"Effect of low-power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts","volume":"31","author":"Pereira","year":"2002","journal-title":"Lasers Surg. Med."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1002\/lsm.22441","article-title":"Low intensity 635\u2009nm diode laser irradiation inhibits fibroblast-myofibroblast transition reducing TRPC1 channel expression\/activity: New perspectives for tissue fibrosis treatment","volume":"48","author":"Sassoli","year":"2016","journal-title":"Lasers Surg. Med."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.actbio.2016.06.022","article-title":"Multipotent mesenchymal stromal cell sheet therapy for bisphosphonate-related osteonecrosis of the jaw in a rat model","volume":"42","author":"Kaibuchi","year":"2016","journal-title":"Acta Biomater."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1002\/lsm.20008","article-title":"Effect of low-power laser irradiation on protein synthesis and ultrastructure of human gingival fibroblasts","volume":"34","author":"Marques","year":"2004","journal-title":"Lasers Surg. Med."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1097\/00000637-198309000-00007","article-title":"Effects of the Nd:YAG laser on DNA synthesis and collagen production in human skin fibroblast cultures","volume":"11","author":"Castro","year":"1983","journal-title":"Ann. Plast Surg."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1002\/lsm.1900030403","article-title":"Nonthermal effects of ND:YAG laser on biological functions of human skin fibroblasts in culture","volume":"3","author":"Abergel","year":"1984","journal-title":"Lasers Surg. Med."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1111\/j.1524-4725.1998.tb04165.x","article-title":"The Q-switched neodymium (Nd):YAG laser with quadruple frequency. Clinical histological evaluation of facial resurfacing using different wavelengths","volume":"24","author":"Cisneros","year":"1998","journal-title":"Derm. Surg."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1762","DOI":"10.1038\/sj.jid.5700765","article-title":"Regulation of MMP-2 gene transcription in dermal wounds","volume":"127","author":"Jansen","year":"2007","journal-title":"J. Investig. Derm."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1007\/s10103-018-2635-1","article-title":"Q-switched 1064 nm Nd-Yag nanosecond laser effects on skin barrier function and on molecular rejuvenation markers in keratinocyte-fibroblasts interaction","volume":"34","author":"Perfetto","year":"2019","journal-title":"Lasers Med. Sci."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1089\/pho.2012.3221","article-title":"Investigation of the 1064\u2009nm Q-switched Nd:YAG laser on collagen expression in an animal model","volume":"30","author":"Ye","year":"2012","journal-title":"Photomed. Laser Surg."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1007\/s10103-009-0657-4","article-title":"Effects of the 532-nm and 1064-nm Q-switched Nd: YAG lasers on collagen turnover of cultured human skin fibroblasts: A comparative study","volume":"25","author":"Dang","year":"2010","journal-title":"Lasers Med. Sci."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1089\/clm.1996.14.67","article-title":"Clinical assessments of the erbium:YAG laser for soft tissue surgery and scaling","volume":"14","author":"Watanabe","year":"1996","journal-title":"J. Clin. Laser Med. Surg."},{"key":"ref_117","first-page":"674","article-title":"Current status of clinical laser applications in periodontal therapy","volume":"56","author":"Aoki","year":"2008","journal-title":"Gen Dent"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1902\/jop.2005.76.2.187","article-title":"Effect of low-level Er:YAG laser irradiation on cultured human gingival fibroblasts","volume":"76","author":"Pourzarandian","year":"2005","journal-title":"J. Periodontol."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1111\/j.1600-0765.2005.00789.x","article-title":"Er:YAG laser irradiation increases prostaglandin E production via the induction of cyclooxygenase-2 mRNA in human gingival fibroblasts","volume":"40","author":"Pourzarandian","year":"2005","journal-title":"J. Periodontal Res."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1007\/s10103-014-1691-4","article-title":"Increased cell proliferation and differential protein expression induced by low-level Er:YAG laser irradiation in human gingival fibroblasts: Proteomic analysis","volume":"30","author":"Ogita","year":"2015","journal-title":"Lasers Med. Sci."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"201700157","DOI":"10.1002\/jbio.201700157","article-title":"Biological effects of Er:YAG laser irradiation on the proliferation of primary human gingival fibroblasts","volume":"11","author":"Kong","year":"2018","journal-title":"J. Biophotonics"},{"key":"ref_122","first-page":"251","article-title":"In Vitro Study of Er:YAG and Er, Cr:YSGG Laser Irradiation on Human Gingival Fibroblast Cell Line","volume":"54","author":"Torshabi","year":"2016","journal-title":"Acta Med. Iran"},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1007\/s10103-020-03079-y","article-title":"Examination of the effect of combined use of Er:YAG laser irradiation and mechanical force loading on bone metabolism using primary human gingival fibroblasts","volume":"35","author":"Tsuka","year":"2020","journal-title":"Lasers Med. Sci."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1054\/ijom.2001.0189","article-title":"Interventional laser surgery: An effective surgical and diagnostic tool in oral precancer management","volume":"31","author":"Thomson","year":"2002","journal-title":"Int. J. Oral. Maxillofac. Surg."},{"key":"ref_125","first-page":"787","article-title":"Effect of CO\u2082 laser irradiation on hormesis induction in human pulp and periodontal ligament fibroblasts","volume":"25","author":"Iwasaka","year":"2011","journal-title":"In Vivo"},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1111\/j.1600-0765.2004.00751.x","article-title":"Behavior of human periodontal ligament cells on CO2 laser irradiated dentinal root surfaces: An in vitro study","volume":"39","author":"Pant","year":"2004","journal-title":"J. Periodontal Res."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1002\/lsm.20712","article-title":"Remodeling of the rat gingiva induced by CO2 laser coagulation mode","volume":"40","author":"Yamasaki","year":"2008","journal-title":"Lasers Surg. Med."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1097\/00006534-200005000-00019","article-title":"The effect of superpulsed carbon dioxide laser energy on keloid and normal dermal fibroblast secretion of growth factors: A serum-free study","volume":"105","author":"Nowak","year":"2000","journal-title":"Plast Reconstr. Surg."},{"key":"ref_129","doi-asserted-by":"crossref","unstructured":"Shingyochi, Y., Kanazawa, S., Tajima, S., Tanaka, R., Mizuno, H., and Tobita, M. (2017). A Low-Level Carbon Dioxide Laser Promotes Fibroblast Proliferation and Migration through Activation of Akt, ERK, and JNK. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0168937"},{"key":"ref_130","first-page":"493","article-title":"Carcinogenic potential of in vitro carbon dioxide laser exposure of fibroblast","volume":"61","author":"Apfelberg","year":"1983","journal-title":"Obs. Gynecol."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/prd.12027","article-title":"Role of integrins in the periodontal ligament: Organizers and facilitators","volume":"63","author":"Barczyk","year":"2013","journal-title":"Periodontoloy 2000"},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1038\/ijos.2013.38","article-title":"Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate","volume":"5","author":"Wu","year":"2013","journal-title":"Int. J. Oral. Sci."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"075602","DOI":"10.1088\/1612-2011\/11\/7\/075602","article-title":"A low-level diode laser therapy reduces the lipopolysaccharide (LPS)-induced periodontal ligament cell inflammation","volume":"11","author":"Huang","year":"2014","journal-title":"Laser Phys. Lett."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1002\/lsm.20871","article-title":"Effect of Ga-Al-As laser irradiation on COX-2 and cPLA2-alpha expression in compressed human periodontal ligament cells","volume":"42","author":"Mayahara","year":"2010","journal-title":"Lasers Surg. Med."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1177\/00220345950740071001","article-title":"Inhibition of prostaglandin E2 and interleukin 1-beta production by low-power laser irradiation in stretched human periodontal ligament cells","volume":"74","author":"Shimizu","year":"1995","journal-title":"J. Dent Res."},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1002\/(SICI)1096-9101(1997)21:5<456::AID-LSM7>3.0.CO;2-P","article-title":"Low-energy diode laser irradiation reduced plasminogen activator activity in human periodontal ligament cells","volume":"21","author":"Ozawa","year":"1997","journal-title":"Lasers Surg. Med."},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1089\/pho.2012.3359","article-title":"Low-level laser effects on simulated orthodontic tension side periodontal ligament cells","volume":"31","author":"Huang","year":"2013","journal-title":"Photomed. Laser Surg."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"105","DOI":"10.5051\/jpis.2010.40.3.105","article-title":"Biological effects of a semiconductor diode laser on human periodontal ligament fibroblasts","volume":"40","author":"Choi","year":"2010","journal-title":"J. Periodontal. Implant Sci."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"213","DOI":"10.4103\/1735-3327.284732","article-title":"The effect of laser irradiation and doxycycline application on the production of matrix metalloproteinase-8 and collagen I from cultured human periodontal ligament cells","volume":"17","author":"Dehdashtizadeh","year":"2020","journal-title":"Dent. Res. J. Isfahan"},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1034\/j.1600-051X.2003.00001.x","article-title":"Effect of low-level GaAlAs laser irradiation on the proliferation rate of human periodontal ligament fibroblasts: An in vitro study","volume":"30","author":"Kreisler","year":"2003","journal-title":"J. Clin. Periodontol."},{"key":"ref_141","doi-asserted-by":"crossref","unstructured":"Lin, T., Yu, C.C., Liu, C.M., Hsieh, P.L., Liao, Y.W., Yu, C.H., and Chen, C.J. (2020). Er:YAG laser promotes proliferation and wound healing capacity of human periodontal ligament fibroblasts through Galectin-7 induction. J. Med. Assoc.","DOI":"10.1016\/j.jfma.2020.06.005"},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1089\/pho.2008.2446","article-title":"Laser photobiomodulation of wound healing: A review of experimental studies in mouse and rat animal models","volume":"28","author":"Peplow","year":"2010","journal-title":"Photomed. Laser Surg."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/j.1600-0757.2009.00301.x","article-title":"The impact of laser application on periodontal and peri-implant wound healing","volume":"51","author":"Schwarz","year":"2009","journal-title":"Periodontology 2000"},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1146\/annurev-physiol-022516-034356","article-title":"Mechanisms of Organ Injury and Repair by Macrophages","volume":"79","author":"Vannella","year":"2017","journal-title":"Annu. Rev. Physiol."},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1007\/s10103-018-2623-5","article-title":"Photobiomodulation with 808-nm diode laser light promotes wound healing of human endothelial cells through increased reactive oxygen species production stimulating mitochondrial oxidative phosphorylation","volume":"34","author":"Amaroli","year":"2019","journal-title":"Lasers Med. Sci."},{"key":"ref_146","first-page":"313","article-title":"Influence of low-power laser illumination on pro-inflammatory response in human endothelial cells stimulated with interleukin-1a","volume":"27","author":"Protasiewicz","year":"2013","journal-title":"In Vivo"},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1046\/j.1365-2133.2003.05070.x","article-title":"Direct stimulatory effect of low-intensity 670 nm laser irradiation on human endothelial cell proliferation","volume":"148","author":"Schindl","year":"2003","journal-title":"Br. J. Derm."},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s10103-014-1650-0","article-title":"Effect of LLLT on endothelial cells culture","volume":"30","author":"Drela","year":"2015","journal-title":"Lasers Med. Sci."},{"key":"ref_149","first-page":"2380","article-title":"Heat shock protein 70 expression induced by diode laser irradiation on choroid-retinal endothelial cells in vitro","volume":"18","author":"Du","year":"2012","journal-title":"Mol. Vis."},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"51","DOI":"10.21873\/invivo.11024","article-title":"Gene Expression Analysis of Cultured Rat-Endothelial Cells after Nd:YAG Laser Irradiation by Affymetrix GeneChip Array","volume":"31","author":"Masuda","year":"2017","journal-title":"In Vivo"},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1902\/jop.2009.080648","article-title":"In vitro evaluation of the effects of low-intensity Nd:YAG laser irradiation on the inflammatory reaction elicited by bacterial lipopolysaccharide adherent to titanium dental implants","volume":"80","author":"Giannelli","year":"2009","journal-title":"J. Periodontol."},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1034\/j.1600-0757.2000.2240105.x","article-title":"Molecular and cell biology of cementum","volume":"24","author":"Saygin","year":"2000","journal-title":"Periodontoloy 2000"},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1007\/s10103-017-2192-z","article-title":"Biostimulation with diode laser positively regulates cementoblast functions, in vitro","volume":"32","author":"Bozkurt","year":"2017","journal-title":"Lasers Med. Sci."},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1172\/JCI39104","article-title":"The basics of epithelial-mesenchymal transition","volume":"119","author":"Kalluri","year":"2009","journal-title":"J. Clin. Investig."},{"key":"ref_155","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.molmed.2018.01.004","article-title":"Inflammasome, Inflammation, and Tissue Homeostasis","volume":"24","author":"Rathinam","year":"2018","journal-title":"Trends Mol. Med."},{"key":"ref_156","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1007\/s10103-019-02745-0","article-title":"Photobiomodulation therapy modulates epigenetic events and NF-kappaB expression in oral epithelial wound healing","volume":"34","author":"Wagner","year":"2019","journal-title":"Lasers Med. Sci."},{"key":"ref_157","first-page":"223","article-title":"Healing of rat mouth mucosa after irradiation with CO2, Nd:YAG, and CO2-Nd:YAG combination lasers","volume":"102","author":"Luomanen","year":"1994","journal-title":"Scand. J. Dent. Res."},{"key":"ref_158","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1007\/s10103-013-1478-z","article-title":"Gingival tissue healing following Er:YAG laser ablation compared to electrosurgery in rats","volume":"30","author":"Sawabe","year":"2015","journal-title":"Lasers Med. Sci."},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1097\/00042728-200503000-00016","article-title":"Low-level laser therapy for wound healing: Mechanism and efficacy","volume":"31","author":"Posten","year":"2005","journal-title":"Derm. Surg."},{"key":"ref_160","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1002\/(SICI)1096-9101(1998)22:4<212::AID-LSM5>3.0.CO;2-S","article-title":"780 nm low power diode laser irradiation stimulates proliferation of keratinocyte cultures: Involvement of reactive oxygen species","volume":"22","author":"Grossman","year":"1998","journal-title":"Lasers Surg. Med."},{"key":"ref_161","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1007\/s10103-012-1057-8","article-title":"Biostimulatory effect of low-level laser therapy on keratinocytes in vitro","volume":"28","author":"Basso","year":"2013","journal-title":"Lasers Med. Sci."},{"key":"ref_162","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1177\/039463201002300420","article-title":"Inhibition of HSV-1 replication by laser diode-irradiation: Possible mechanism of action","volume":"23","author":"Donnarumma","year":"2010","journal-title":"Int. J. Immunopathol. Pharm."},{"key":"ref_163","first-page":"1024","article-title":"[Low level laser irradiation in the visible spectra induces HeLa cells proliferation]","volume":"32","author":"Yang","year":"2012","journal-title":"Spectrosc. Spectr. Anal."},{"key":"ref_164","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1089\/pho.2004.22.523","article-title":"Cell growth modulation of human cells irradiated in vitro with low-level laser therapy","volume":"22","author":"Mognato","year":"2004","journal-title":"Photomed. Laser Surg."},{"key":"ref_165","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1089\/1080768041223594","article-title":"Laser treatment of cultured retinal pigment epithelial cells-evaluation of the cellular damage in vitro","volume":"20","author":"Varga","year":"2004","journal-title":"J. Ocul. Pharm."},{"key":"ref_166","doi-asserted-by":"crossref","first-page":"2587","DOI":"10.1167\/iovs.04-0920","article-title":"Laser induces apoptosis and ceramide production in human retinal pigment epithelial cells","volume":"46","author":"Barak","year":"2005","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_167","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1159\/000088263","article-title":"Pigment-epithelium-derived factor is upregulated in photocoagulated human retinal pigment epithelial cells","volume":"37","author":"Hattenbach","year":"2005","journal-title":"Ophthalmic Res."},{"key":"ref_168","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1089\/pho.2008.2445","article-title":"The effect of laser irradiation on proliferation of human breast carcinoma, melanoma, and immortalized mammary epithelial cells","volume":"28","author":"Powell","year":"2010","journal-title":"Photomed. Laser Surg."},{"key":"ref_169","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1111\/jre.12399","article-title":"Laser-activated transforming growth factor-\u03b21 induces human \u03b2-defensin 2: Implications for laser therapies for periodontitis and peri-implantitis","volume":"52","author":"Tang","year":"2017","journal-title":"J. Periodontal. Res."},{"key":"ref_170","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1007\/s10103-013-1292-7","article-title":"High-frequency low-level diode laser irradiation promotes proliferation and migration of primary cultured human gingival epithelial cells","volume":"29","author":"Ejiri","year":"2014","journal-title":"Lasers Med. Sci."},{"key":"ref_171","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1111\/jre.12042","article-title":"Antimicrobial photodynamic therapy using a diode laser with a potential new photosensitizer, indocyanine green-loaded nanospheres, may be effective for the clearance of Porphyromonas gingivalis","volume":"48","author":"Nagahara","year":"2013","journal-title":"J. Periodontal. Res."},{"key":"ref_172","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1007\/s10103-013-1439-6","article-title":"Irradiation with a low-level diode laser induces the developmental endothelial locus-1 gene and reduces proinflammatory cytokines in epithelial cells","volume":"29","author":"Fujimura","year":"2014","journal-title":"Lasers Med. Sci"},{"key":"ref_173","first-page":"8","article-title":"Low-power carbon dioxide laser irradiation reduces sclerostin expression, but stimulates Dmp-1 expression in osteocyte-like cells of rats","volume":"3","author":"Yokose","year":"2013","journal-title":"J. Bio-Integ."},{"key":"ref_174","doi-asserted-by":"crossref","unstructured":"Ohsugi, Y., Katagiri, S., Hirota, T., Niimi, H., Hatasa, M., Watanabe, K., Shimohira, T., Mizutani, K., Kitazawa, M., and Matsuzawa, A. (2020). Laser irradiation decreases sclerostin expression in bone and osteogenic cells. Faseb. J.","DOI":"10.1096\/fj.202001032R"},{"key":"ref_175","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1210\/edrv.20.3.0367","article-title":"Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families","volume":"20","author":"Suda","year":"1999","journal-title":"Endocr. Rev."},{"key":"ref_176","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1007\/s10103-005-0368-4","article-title":"Low-energy irradiation stimulates formation of osteoclast-like cells via RANK expression in vitro","volume":"21","author":"Aihara","year":"2006","journal-title":"Lasers Med. Sci."},{"key":"ref_177","unstructured":"Atala, A.L.R. (2012). Handbook of Stem Cells, Academic Press."},{"key":"ref_178","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s10103-007-0479-1","article-title":"The effect of low level laser irradiation on adult human adipose derived stem cells","volume":"23","author":"Mvula","year":"2008","journal-title":"Lasers Med. Sci."},{"key":"ref_179","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0140-6736(04)16627-0","article-title":"Investigation of multipotent postnatal stem cells from human periodontal ligament","volume":"364","author":"Seo","year":"2004","journal-title":"Lancet"},{"key":"ref_180","doi-asserted-by":"crossref","first-page":"13625","DOI":"10.1073\/pnas.240309797","article-title":"Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo","volume":"97","author":"Gronthos","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_181","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1146\/annurev.med.58.082405.095329","article-title":"Progress and potential for regenerative medicine","volume":"58","author":"Gurtner","year":"2007","journal-title":"Annu. Rev. Med."},{"key":"ref_182","doi-asserted-by":"crossref","first-page":"111658","DOI":"10.1016\/j.jphotobiol.2019.111658","article-title":"Photobiomodulation with 630 plus 810nm wavelengths induce more in vitro cell viability of human adipose stem cells than human bone marrow-derived stem cells","volume":"201","author":"Zare","year":"2019","journal-title":"J. Photochem. Photobiol. BBiol."},{"key":"ref_183","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1007\/s10103-011-0995-x","article-title":"Effects of low-level laser irradiation on mesenchymal stem cell proliferation: A microarray analysis","volume":"27","author":"Wu","year":"2012","journal-title":"Lasers Med. Sci."},{"key":"ref_184","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1002\/lsm.20709","article-title":"In vitro effects of low-level laser irradiation for bone marrow mesenchymal stem cells: Proliferation, growth factors secretion and myogenic differentiation","volume":"40","author":"Hou","year":"2008","journal-title":"Lasers Surg. Med."},{"key":"ref_185","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1002\/jcp.24119","article-title":"Photoactivation of bone marrow mesenchymal stromal cells with diode laser: Effects and mechanisms of action","volume":"228","author":"Giannelli","year":"2013","journal-title":"J. Cell. Physiol."},{"key":"ref_186","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1007\/s10103-020-03040-z","article-title":"Photobiomodulation with 808-nm diode laser enhances gingival wound healing by promoting migration of human gingival mesenchymal stem cells via ROS\/JNK\/NF-kappaB\/MMP-1 pathway","volume":"35","author":"Feng","year":"2020","journal-title":"Lasers Med. Sci."},{"key":"ref_187","doi-asserted-by":"crossref","first-page":"123","DOI":"10.3389\/fphys.2018.00123","article-title":"The Effects of Photobiomodulation of 808 nm Diode Laser Therapy at Higher Fluence on the in Vitro Osteogenic Differentiation of Bone Marrow Stromal Cells","volume":"9","author":"Amaroli","year":"2018","journal-title":"Front. Physiol."},{"key":"ref_188","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1002\/lsm.20791","article-title":"In vitro effect of carboplatin, cytarabine, paclitaxel, vincristine, and low-power laser irradiation on murine mesenchymal stem cells","volume":"41","author":"Balogh","year":"2009","journal-title":"Lasers Surg. Med."},{"key":"ref_189","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1007\/s10103-018-2637-z","article-title":"Short-term evaluation of photobiomodulation therapy on the proliferation and undifferentiated status of dental pulp stem cells","volume":"34","author":"Ferreira","year":"2019","journal-title":"Lasers Med. Sci."},{"key":"ref_190","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jphotobiol.2019.03.017","article-title":"Effects of photobiomodulation therapy on the extracellular matrix of human dental pulp cell sheets","volume":"194","author":"Garrido","year":"2019","journal-title":"J. Photochem. Photobiol. BBiol."},{"key":"ref_191","doi-asserted-by":"crossref","first-page":"111742","DOI":"10.1016\/j.jphotobiol.2019.111742","article-title":"Dopaminergic induction of human dental pulp stem cells by photobiomodulation: Comparison of 660 nm laser light and polychromatic light in the nir","volume":"204","author":"Yurtsever","year":"2020","journal-title":"J. Photochem. Photobiol. BBiol."},{"key":"ref_192","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/s10103-018-2673-8","article-title":"Low-level laser irradiation modulates the proliferation and the osteogenic differentiation of bone marrow mesenchymal stem cells under healthy and inflammatory condition","volume":"34","author":"Wang","year":"2019","journal-title":"Lasers Med. Sci."},{"key":"ref_193","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1089\/pho.2017.4344","article-title":"In Vitro Effects of High-Intensity Laser Photobiomodulation on Equine Bone Marrow-Derived Mesenchymal Stem Cell Viability and Cytokine Expression","volume":"36","author":"Peat","year":"2018","journal-title":"Photomed. Laser Surg."},{"key":"ref_194","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1002\/lsm.22083","article-title":"Visible 532 nm laser irradiation of human adipose tissue-derived stem cells: Effect on proliferation rates, mitochondria membrane potential and autofluorescence","volume":"44","author":"Anwer","year":"2012","journal-title":"Lasers Surg. Med."},{"key":"ref_195","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/s10103-016-2093-6","article-title":"CO2 laser increases the regenerative capacity of human adipose-derived stem cells by a mechanism involving the redox state and enhanced secretion of pro-angiogenic molecules","volume":"32","author":"Constantin","year":"2017","journal-title":"Lasers Med. Sci."},{"key":"ref_196","first-page":"38","article-title":"Periodontal regeneration with autologous periodontal ligament-derived cell sheets\u2014A safety and efficacy study in ten patients","volume":"9","author":"Iwata","year":"2018","journal-title":"Regen"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/23\/9002\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:38:01Z","timestamp":1760179081000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/23\/9002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,26]]},"references-count":196,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["ijms21239002"],"URL":"https:\/\/doi.org\/10.3390\/ijms21239002","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,26]]}}}