{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T05:06:36Z","timestamp":1788584796350,"version":"build-2803163510"},"reference-count":67,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T00:00:00Z","timestamp":1606435200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["133605"],"award-info":[{"award-number":["133605"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"publisher","award":["230552"],"award-info":[{"award-number":["230552"]}],"id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>(1) Background: The atopic march is defined by the increased prevalence of allergic diseases after atopic dermatitis onset. In fact, atopic dermatitis is believed to play an important role in allergen sensitization via the damaged skin barrier, leading to allergic diseases such as allergic asthma and allergic rhinitis. The eosinophil, a pro-inflammatory cell that contributes to epithelial damage, is one of the various cells recruited in the inflammatory reactions characterizing these diseases. Few studies were conducted on the transcriptome of this cell type and even less on their specific microRNA (miRNA) profile, which could modulate pathogenesis of allergic diseases and clinical manifestations post-transcriptionally. Actually, their implication in allergic diseases is not fully understood, but they are believed to play a role in inflammation-related patterns and epithelial cell proliferation. (2) Methods: Next-generation sequencing was performed on RNA samples from eosinophils of individuals with atopic dermatitis, atopy, allergic rhinitis and asthma to obtain differential counts of primary miRNA (pri-miRNA); these were also analyzed for asthma-related phenotypes such as forced expiratory volume in one second (FEV1), immunoglobulin E (IgE) and provocative concentration of methacholine inducing a 20% fall in forced expiratory volume in 1 s (PC20) levels, as well as FEV1 to forced vital capacity (FEV1\/FVC) ratio. (3) Results: Eighteen miRNAs from eosinophils were identified to be significantly different between affected individuals and unaffected ones. Based on counts from these miRNAs, individuals were then clustered into groups using Ward\u2019s method on Euclidian distances. Groups were found to be explained by asthma diagnosis, familial history of respiratory diseases and allergic rhinitis as well as neutrophil counts. (4) Conclusions: The 18 differential miRNA counts for the studying phenotypes allow a better understanding of the epigenetic mechanisms underlying the development of the allergic diseases included in the atopic march.<\/jats:p>","DOI":"10.3390\/ijms21239011","type":"journal-article","created":{"date-parts":[[2020,11,27]],"date-time":"2020-11-27T09:16:49Z","timestamp":1606468609000},"page":"9011","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Eosinophil microRNAs Play a Regulatory Role in Allergic Diseases Included in the Atopic March"],"prefix":"10.3390","volume":"21","author":[{"given":"\u00c9mile","family":"B\u00e9langer","sequence":"first","affiliation":[{"name":"D\u00e9partement des Sciences Fondamentales, Universit\u00e9 du Qu\u00e9bec \u00e0 Chicoutimi, Saguenay, QC G7H 2B1, Canada"},{"name":"Centre Intersectoriel en Sant\u00e9 Durable, Universit\u00e9 du Qu\u00e9bec \u00e0 Chicoutimi, Saguenay, QC G7H 2B1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Marie","family":"Madore","sequence":"additional","affiliation":[{"name":"D\u00e9partement des Sciences Fondamentales, Universit\u00e9 du Qu\u00e9bec \u00e0 Chicoutimi, Saguenay, QC G7H 2B1, Canada"},{"name":"Centre Intersectoriel en Sant\u00e9 Durable, Universit\u00e9 du Qu\u00e9bec \u00e0 Chicoutimi, Saguenay, QC G7H 2B1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Marie","family":"Boucher-Lafleur","sequence":"additional","affiliation":[{"name":"D\u00e9partement des Sciences Fondamentales, Universit\u00e9 du Qu\u00e9bec \u00e0 Chicoutimi, Saguenay, QC G7H 2B1, Canada"},{"name":"Centre Intersectoriel en Sant\u00e9 Durable, Universit\u00e9 du Qu\u00e9bec \u00e0 Chicoutimi, Saguenay, QC G7H 2B1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Michelle","family":"Simon","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, McGill University, Montreal, QC H3A 0C7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony","family":"Kwan","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, McGill University, Montreal, QC H3A 0C7, Canada"},{"name":"McGill University and G\u00e9nome Qu\u00e9bec Innovation Center, Montreal, QC H3A 0G1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomi","family":"Pastinen","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, McGill University, Montreal, QC H3A 0C7, Canada"},{"name":"McGill University and G\u00e9nome Qu\u00e9bec Innovation Center, Montreal, QC H3A 0G1, Canada"},{"name":"Center for Pediatric Genomic Medicine, Kansas City, MO 64108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5526-9945","authenticated-orcid":false,"given":"Catherine","family":"Laprise","sequence":"additional","affiliation":[{"name":"D\u00e9partement des Sciences Fondamentales, Universit\u00e9 du Qu\u00e9bec \u00e0 Chicoutimi, Saguenay, QC G7H 2B1, Canada"},{"name":"Centre Intersectoriel en Sant\u00e9 Durable, Universit\u00e9 du Qu\u00e9bec \u00e0 Chicoutimi, Saguenay, QC G7H 2B1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.jaci.2015.03.048","article-title":"The allergy epidemics: 1870\u20132010","volume":"136","year":"2015","journal-title":"J. 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