{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T23:13:46Z","timestamp":1781478826951,"version":"3.54.1"},"reference-count":36,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T00:00:00Z","timestamp":1607472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Natural Sciences Foundation of China","award":["81974247 and 916422042"],"award-info":[{"award-number":["81974247 and 916422042"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Immunoglobulin (Ig\u03ba) has been reported to be expressed in sorted liver epithelial cells of \u03bcMT mice, and the sequence characteristics of hepatocyte-derived Ig\u03ba were different from those of classical B-cell-derived Ig\u03ba. However, the physiological function of hepatocyte-derived Ig\u03ba is still unclear. The expression of Ig\u03ba was firstly identified in primary hepatocytes and normal liver cell line (NCTC1469), and hepatocyte-derived Ig\u03ba expression was elevated and displayed unique localization in hepatocytes of concanavalin A (ConA)-induced hepatitis model. Moreover, Ig\u03ba knockout mice were more sensitive to ConA-induced hepatitis and had higher serum aspartate aminotransferase (AST) levels, more severe histological injury and a greater number of terminal deoxynucleotide transferase-mediated deoxyuridine triphosphate nick end-labeling (TUNEL)-positive cells as compared with littermate controls. Furthermore, knockdown of Ig\u03ba in primary hepatocytes and NCTC1469 cells led to accelerated activation of the mitochondrial death pathway and caspase-3 cleavage in vitro, which might be related to inhibition of NF-\u03baB signaling pathway and activation of JNK via the cytoskeleton dynamics. Taken together, these results indicate that hepatocyte-derived Ig\u03ba mediates cellular resistance to ConA-induced liver injury by inhibiting activation of caspase-3 and the mitochondrial death pathway, suggesting that Ig\u03ba plays an important role in hepatocyte survival and exerts a protective effect against ConA-induced liver injury in mice.<\/jats:p>","DOI":"10.3390\/ijms21249379","type":"journal-article","created":{"date-parts":[[2020,12,9]],"date-time":"2020-12-09T09:17:58Z","timestamp":1607505478000},"page":"9379","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Hepatocyte-Derived Ig\u03ba Exerts a Protective Effect against ConA-Induced Acute Liver Injury"],"prefix":"10.3390","volume":"21","author":[{"given":"Sha","family":"Yin","sequence":"first","affiliation":[{"name":"Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China"},{"name":"NHC Key Laboratory of Medical Immunology, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianwen","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China"},{"name":"NHC Key Laboratory of Medical Immunology, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwei","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China"},{"name":"NHC Key Laboratory of Medical Immunology, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China"},{"name":"NHC Key Laboratory of Medical Immunology, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China"},{"name":"NHC Key Laboratory of Medical Immunology, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China"},{"name":"NHC Key Laboratory of Medical Immunology, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0814-838X","authenticated-orcid":false,"given":"Jing","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100191, China"},{"name":"NHC Key Laboratory of Medical Immunology, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,9]]},"reference":[{"key":"ref_1","first-page":"6488","article-title":"Human epithelial cancers secrete immunoglobulin g with unidentified specificity to promote growth and survival of tumor cells","volume":"63","author":"Qiu","year":"2003","journal-title":"Cancer Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"40081","DOI":"10.18632\/oncotarget.5542","article-title":"Aberrant high expression of immunoglobulin G in epithelial stem\/progenitor-like cells contributes to tumor initiation and metastasis","volume":"6","author":"Liao","year":"2015","journal-title":"Oncotarget"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1604","DOI":"10.1016\/j.biocel.2007.12.004","article-title":"Expression of immunoglobulin gene with classical V-(D)-J rearrangement in mouse brain neurons","volume":"40","author":"Huang","year":"2008","journal-title":"Int. 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