{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T04:55:44Z","timestamp":1789275344205,"version":"build-2803163510"},"reference-count":78,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T00:00:00Z","timestamp":1608163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","award":["APVV-17-0369"],"award-info":[{"award-number":["APVV-17-0369"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006108","name":"KEGA","doi-asserted-by":"publisher","award":["016UK-4\/2018"],"award-info":[{"award-number":["016UK-4\/2018"]}],"id":[{"id":"10.13039\/501100006108","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The European Regional Development Fund","award":["No. ERDF ITMS: 26240220044."],"award-info":[{"award-number":["No. ERDF ITMS: 26240220044."]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Despite outstanding advances in diagnosis and the treatment of primary uveal melanoma (UM), nearly 50% of UM patients develop metastases via hematogenous dissemination, driven by the epithelial-mesenchymal transition (EMT). Despite the failure in UM to date, a liquid biopsy may offer a feasible non-invasive approach for monitoring metastatic disease progression and addressing protracted dormancy. To detect circulating tumor cells (CTCs) in UM patients, we evaluated the mRNA expression of EMT-associated transcription factors in CD45-depleted blood fraction, using qRT-PCR. ddPCR was employed to assess UM-specific GNA11, GNAQ, PLC\u03b24, and CYSLTR2 mutations in plasma DNA. Moreover, microarray analysis was performed on total RNA isolated from tumor tissues to estimate the prognostic value of EMT-associated gene expression. In total, 42 primary UM and 11 metastatic patients were enrolled. All CD45-depleted samples were negative for CTC when compared to the peripheral blood fraction of 60 healthy controls. Tumor-specific mutations were detected in the plasma of 21.4% patients, merely, in 9.4% of primary UM, while 54.5% in metastatic patients. Unsupervised hierarchical clustering of differentially expressed EMT genes showed significant differences between monosomy 3 and disomy 3 tumors. Newly identified genes can serve as non-invasive prognostic biomarkers that can support therapeutic decisions.<\/jats:p>","DOI":"10.3390\/ijms21249651","type":"journal-article","created":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T21:21:49Z","timestamp":1608240109000},"page":"9651","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Monosomy 3 Influences Epithelial-Mesenchymal Transition Gene Expression in Uveal Melanoma Patients; Consequences for Liquid Biopsy"],"prefix":"10.3390","volume":"21","author":[{"given":"Andrea","family":"Soltysova","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovicova 6, 841 04 Bratislava, Slovakia"},{"name":"Institute for Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Sedlackova","sequence":"additional","affiliation":[{"name":"Comenius University Science Park, Ilkovicova 8, 841 04 Bratislava, Slovakia"},{"name":"Geneton Ltd., Ilkovicova 8, 841 04 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Dvorska","sequence":"additional","affiliation":[{"name":"Laboratory of Genomics and Prenatal Diagnosis, Biomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 10701\/4A, 03 601 Martin, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Jasek","sequence":"additional","affiliation":[{"name":"Laboratory of Genomics and Prenatal Diagnosis, Biomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 10701\/4A, 03 601 Martin, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pooneh","family":"Chokhachi Baradaran","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia"},{"name":"Department of Genetics, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovicova 6, 841 04 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7081-0602","authenticated-orcid":false,"given":"Viera","family":"Horvathova Kajabova","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Demkova","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2466-9126","authenticated-orcid":false,"given":"Verona","family":"Buocikova","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terezia","family":"Kurucova","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovicova 6, 841 04 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2926-1664","authenticated-orcid":false,"given":"Darina","family":"Lyskova","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine, Comenius University in Bratislava, Ruzinovska 6, 821 01 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5689-892X","authenticated-orcid":false,"given":"Alena","family":"Furdova","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine, Comenius University in Bratislava, Ruzinovska 6, 821 01 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Minarik","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 4, 811 08, Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8170-1850","authenticated-orcid":false,"given":"Pavel","family":"Babal","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 4, 811 08 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"Dankova","sequence":"additional","affiliation":[{"name":"Laboratory of Genomics and Prenatal Diagnosis, Biomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 10701\/4A, 03 601 Martin, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4906-5652","authenticated-orcid":false,"given":"Bozena","family":"Smolkova","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,17]]},"reference":[{"key":"ref_1","first-page":"1000","article-title":"Incidence of noncutaneous melanomas in the US","volume":"103","author":"McLaughlin","year":"2005","journal-title":"Cancer Interdiscip. Int. J. Am. Cancer Soc."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Murray, T.G., and Boldt, H.C. (2014). Ocular Melanoma: Advances in Diagnostic and Therapeutic Strategies, Future Medicine Limited.","DOI":"10.2217\/9781780844268"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.ophtha.2010.05.023","article-title":"Prognosis of uveal melanoma in 500 cases using genetic testing of fine-needle aspiration biopsy specimens","volume":"118","author":"Shields","year":"2011","journal-title":"Ophthalmology"},{"key":"ref_4","first-page":"29","article-title":"Clinical management of uveal and conjunctival melanoma","volume":"30","author":"Blum","year":"2016","journal-title":"Oncol. (Williston Park)"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Kaliki, S., Shields, C.L., and Shields, J.A. (2015). Uveal melanoma: Estimating prognosis. Indian J. Ophthalmol., 63.","DOI":"10.4103\/0301-4738.154367"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Versluis, M., de Lange, M.J., van Pelt, S.I., Ruivenkamp, C.A., Kroes, W.G., Cao, J., Jager, M.J., Luyten, G.P., and van der Velden, P.A. (2015). Digital PCR validates 8q dosage as prognostic tool in uveal melanoma. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0116371"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1038\/bjc.2014.417","article-title":"Lack of BAP1 protein expression in uveal melanoma is associated with increased metastatic risk and has utility in routine prognostic testing","volume":"111","author":"Kalirai","year":"2014","journal-title":"Br. J. Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1596","DOI":"10.1016\/j.ophtha.2012.02.017","article-title":"Collaborative Ocular Oncology Group report number 1: Prospective validation of a multi-gene prognostic assay in uveal melanoma","volume":"119","author":"Onken","year":"2012","journal-title":"Ophthalmology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1038\/s41588-019-0440-9","article-title":"The genetic evolution of metastatic uveal melanoma","volume":"51","author":"Shain","year":"2019","journal-title":"Nat. Genet."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1038\/35000034","article-title":"The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells","volume":"2","author":"Batlle","year":"2000","journal-title":"Nat. Cell Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1038\/sj.onc.1208429","article-title":"DeltaEF1 is a transcriptional repressor of E-cadherin and regulates epithelial plasticity in breast cancer cells","volume":"24","author":"Eger","year":"2005","journal-title":"Oncogene"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1016\/S1097-2765(01)00260-X","article-title":"The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion","volume":"7","author":"Comijn","year":"2001","journal-title":"Mol. Cell"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1016\/j.cell.2004.06.006","article-title":"Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis","volume":"117","author":"Yang","year":"2004","journal-title":"Cell"},{"key":"ref_14","unstructured":"Asnaghi, L., Gezgin, G., Tripathy, A., Handa, J.T., Merbs, S.L., van der Velden, P.A., Jager, M.J., Harbour, J.W., and Eberhart, C.G. (2015). EMT-associated factors promote invasive properties of uveal melanoma cells. Mol. Vis., 21."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1443","DOI":"10.1016\/S0161-6420(00)00182-2","article-title":"Tumor doubling times in metastatic malignant melanoma of the uvea: Tumor progression before and after treatment","volume":"107","author":"Eskelin","year":"2000","journal-title":"Ophthalmology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1038\/nrc3066","article-title":"Cell-free nucleic acids as biomarkers in cancer patients","volume":"11","author":"Schwarzenbach","year":"2011","journal-title":"Nat. Rev. Cancer"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Park, J.J., Diefenbach, R.J., Byrne, N., Kefford, R., Long, G.V., Scolyer, R.A., Gray, E., Carlino, M.S., and Rizos, H. (2020). Circulating Tumor DNA (ctDNA) in Patients (pts) with Metastatic Uveal Melanoma (UM) Treated with Protein Kinase C Inhibitor (PKCi), American Society of Clinical Oncology.","DOI":"10.1200\/JCO.2020.38.15_suppl.e22054"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3552","DOI":"10.1158\/1078-0432.CCR-11-3086","article-title":"Identification of unique MEK-dependent genes in GNAQ mutant uveal melanoma involved in cell growth, tumor cell invasion, and MEK resistance","volume":"18","author":"Ambrosini","year":"2012","journal-title":"Clin. Cancer Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Mego, M., Cholujova, D., Minarik, G., Sedlackova, T., Gronesova, P., Karaba, M., Benca, J., Cingelova, S., Cierna, Z., and Manasova, D. (2016). CXCR4-SDF-1 interaction potentially mediates trafficking of circulating tumor cells in primary breast cancer. Bmc Cancer, 16.","DOI":"10.1186\/s12885-016-2143-2"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Anfuso, C.D., Longo, A., Distefano, A., Amorini, A.M., Salmeri, M., Zangh\u00ec, G., Giallongo, C., Giurdanella, G., and Lupo, G. (2020). Uveal Melanoma Cells Elicit Retinal Pericyte Phenotypical and Biochemical Changes in an in Vitro Model of Coculture. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21155557"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Fei, F., Qu, J., Zhang, M., Li, Y., and Zhang, S. (2017). S100A4 in cancer progression and metastasis: A systematic review. Oncotarget, 8.","DOI":"10.18632\/oncotarget.18016"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4602","DOI":"10.1158\/0008-5472.CAN-05-4196","article-title":"Functional gene expression analysis uncovers phenotypic switch in aggressive uveal melanomas","volume":"66","author":"Onken","year":"2006","journal-title":"Cancer Res."},{"key":"ref_23","first-page":"1","article-title":"Protein tyrosine phosphatase 4A3 (PTP4A3\/PRL-3) promotes the aggressiveness of human uveal melanoma through dephosphorylation of CRMP2","volume":"9","author":"Laura","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1158\/0008-5472.CAN-10-0605","article-title":"High PTP4A3 phosphatase expression correlates with metastatic risk in uveal melanoma patients","volume":"71","author":"Laurent","year":"2011","journal-title":"Cancer Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3922","DOI":"10.1096\/fj.201700180R","article-title":"Human salivary peptide histatin-1 stimulates epithelial and endothelial cell adhesion and barrier function","volume":"31","author":"Hoebe","year":"2017","journal-title":"Faseb J."},{"key":"ref_26","unstructured":"Proen\u00e7a, R.P., Fonseca, C., Goyeneche, A.A., Ito, H., Dias, A., Marques-Neves, C., and Burnier, M.N. (2020). The expression of ALK, RET, ROS1, c-MET, EGFR and IGF-R1 in uveal melanoma and its association to clinicopathological characteristics and prognosis. Investig. Ophthalmol. Vis. Sci., 61."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1515\/BC.2002.076","article-title":"Structural and functional diversity of connexin genes in the mouse and human genome","volume":"383","author":"Willecke","year":"2002","journal-title":"Biol. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.3892\/ijo.2015.3120","article-title":"The collagen receptor uPARAP\/Endo180 in tissue degradation and cancer","volume":"47","author":"Melander","year":"2015","journal-title":"Int. J. Oncol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1007\/s00417-011-1786-6","article-title":"GDF-15: A novel serum marker for metastases in uveal melanoma patients","volume":"250","author":"Suesskind","year":"2012","journal-title":"Graefe\u2019s Arch. Clin. Exp. Ophthalmol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1007\/s00428-003-0883-2","article-title":"Sequence analysis and high-throughput immunhistochemical profiling of KIT (CD 117) expression in uveal melanoma using tissue microarrays","volume":"443","author":"Pache","year":"2003","journal-title":"Virchows Arch."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1002\/path.5384","article-title":"Loss of BAP1 expression is associated with an immunosuppressive microenvironment in uveal melanoma, with implications for immunotherapy development","volume":"250","author":"Figueiredo","year":"2020","journal-title":"J. Pathol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Krishna, Y., Acha-Sagredo, A., Sabat-Po\u015bpiech, D., Kipling, N., Clarke, K., Figueiredo, C.R., Kalirai, H., and Coupland, S.E. (2020). Transcriptome Profiling Reveals New Insights into the Immune Microenvironment and Upregulation of Novel Biomarkers in Metastatic Uveal Melanoma. Cancers, 12.","DOI":"10.3390\/cancers12102832"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Fazilaty, H., Rago, L., Kass Youssef, K., Oca\u00f1a, O.H., Garcia-Asencio, F., Arcas, A., Galceran, J., and Nieto, M.A. (2019). A gene regulatory network to control EMT programs in development and disease. Nat. Commun., 10.","DOI":"10.1038\/s41467-019-13091-8"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Peng, W., Zhang, J., and Liu, J. (2014). URG11 predicts poor prognosis of pancreatic cancer by enhancing epithelial\u2013mesenchymal transition-driven invasion. Med. Oncol., 31.","DOI":"10.1007\/s12032-014-0064-y"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2717","DOI":"10.1002\/cam4.2152","article-title":"Prognostic role of cyclin D2\/D3 in multiple human malignant neoplasms: A systematic review and meta-analysis","volume":"8","author":"Ding","year":"2019","journal-title":"Cancer Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1093\/jb\/mvy008","article-title":"Cell surface chondroitin sulphate proteoglycan 4 (CSPG4) binds to the basement membrane heparan sulphate proteoglycan, perlecan, and is involved in cell adhesion","volume":"163","author":"Tang","year":"2018","journal-title":"J. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Asnaghi, L., Lin, M.H., Lim, K.S., Lim, K.J., Tripathy, A., Wendeborn, M., Merbs, S.L., Handa, J.T., Sodhi, A., and Bar, E.E. (2014). Hypoxia promotes uveal melanoma invasion through enhanced Notch and MAPK activation. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0105372"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"295","DOI":"10.3892\/or.2015.3949","article-title":"Mutant GNAQ promotes cell viability and migration of uveal melanoma cells through the activation of Notch signaling","volume":"34","author":"Liu","year":"2015","journal-title":"Oncol. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e947169","DOI":"10.4161\/21624011.2014.947169","article-title":"Molecular profiling of primary uveal melanomas with tumor-infiltrating lymphocytes","volume":"8","author":"Triozzi","year":"2019","journal-title":"Oncoimmunology"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Souri, Z., Wierenga, A., van Weeghel, C., van Der Velden, P.A., Kroes, W.G., Luyten, G.P., van Der Burg, S.H., Jochemsen, A.G., and Jager, M.J. (2019). Loss of BAP1 Is Associated with Upregulation of the NFkB Pathway and Increased HLA Class I Expression in Uveal Melanoma. Cancers, 11.","DOI":"10.3390\/cancers11081102"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1167\/iovs.05-0422","article-title":"Osteopontin expression and serum levels in metastatic uveal melanoma: A pilot study","volume":"47","author":"Kadkol","year":"2006","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-017-00916-z","article-title":"Deficiency of CCN5\/WISP-2-Driven Program in breast cancer Promotes Cancer Epithelial cells to mesenchymal stem cells and Breast Cancer growth","volume":"7","author":"Das","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1002\/ijc.32245","article-title":"Angiogenin promotes colorectal cancer metastasis via tiRNA production","volume":"145","author":"Li","year":"2019","journal-title":"Int. J. Cancer"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Sun, X., Zhang, Q., Chen, W., Hu, Q., Lou, Y., Fu, Q.-H., Zhang, J.-Y., Chen, Y.-W., Ye, L.-Y., and Wang, Y. (2017). Hook1 inhibits malignancy and epithelial\u2013mesenchymal transition in hepatocellular carcinoma. Tumor Biol., 39.","DOI":"10.1177\/1010428317711098"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1007\/978-1-62703-727-3_22","article-title":"A prognostic test to predict the risk of metastasis in uveal melanoma based on a 15-gene expression profile","volume":"1102","author":"Harbour","year":"2014","journal-title":"Methods Mol. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3544","DOI":"10.1002\/cam4.2226","article-title":"KMT2A histone methyltransferase contributes to colorectal cancer development by promoting cathepsin Z transcriptional activation","volume":"8","author":"Fang","year":"2019","journal-title":"Cancer Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2859","DOI":"10.3892\/or.2013.2776","article-title":"ETV1 induces epithelial to mesenchymal transition in human gastric cancer cells through the upregulation of Snail expression","volume":"30","author":"Li","year":"2013","journal-title":"Oncol. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Pereira, P.R., Odashiro, A.N., Lim, L.-A., Miyamoto, C., Blanco, P.L., Odashiro, M., Maloney, S., De Souza, D.F., and Burnier Jr, M.N. (2013). Current and emerging treatment options for uveal melanoma. Clin. Ophthalmol., 7.","DOI":"10.2147\/OPTH.S28863"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Palmirotta, R., Lovero, D., Cafforio, P., Felici, C., Mannavola, F., Pell\u00e8, E., Quaresmini, D., Tucci, M., and Silvestris, F. (2018). Liquid biopsy of cancer: A multimodal diagnostic tool in clinical oncology. Ther. Adv. Med. Oncol., 10.","DOI":"10.1177\/1758835918794630"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Mattox, A.K., Bettegowda, C., Zhou, S., Papadopoulos, N., Kinzler, K.W., and Vogelstein, B. (2019). Applications of liquid biopsies for cancer. Sci. Transl. Med., 11.","DOI":"10.1126\/scitranslmed.aay1984"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Tura, A., Lueke, J., and Grisanti, S. (2018). Liquid biopsy for uveal melanoma. Exon Publ., 37\u201348.","DOI":"10.15586\/codon.noncutaneousmelanoma.2018.ch3"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1167\/iovs.09-3824","article-title":"Intravascular presence of tumor cells as prognostic parameter in uveal melanoma: A 35-year survey","volume":"51","author":"Ly","year":"2010","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1038\/sj.eye.6702322","article-title":"Identification of circulating malignant cells and its correlation with prognostic factors and treatment in uveal melanoma. A prospective longitudinal study","volume":"21","author":"Callejo","year":"2007","journal-title":"Eye"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1002\/ijc.28436","article-title":"Detection rate and prognostic value of circulating tumor cells and circulating tumor DNA in metastatic uveal melanoma","volume":"134","author":"Bidard","year":"2014","journal-title":"Int. J. Cancer"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1016\/j.ebiom.2015.09.019","article-title":"Arterial Blood, Rather Than Venous Blood, is a Better Source for Circulating Melanoma Cells","volume":"2","author":"Terai","year":"2015","journal-title":"EBioMedicine"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Roszik, J., Gombos, D., Upshaw, J., Sarli, V., Meas, S., Lucci, A., Hall, C., Patel, S., and Anand, K. (2020). Abstract A12: Presence of Circulating Tumor Cells is an Adverse Risk Factor for Early-Stage Uveal Melanoma, AACR.","DOI":"10.1158\/1538-7445.MEL2019-A12"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1200\/PO.17.00279","article-title":"Clinical application of circulating tumor cells and circulating tumor DNA in uveal melanoma","volume":"2","author":"Beasley","year":"2018","journal-title":"Jco Precis. Oncol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/aos.12462","article-title":"Immunomagnetic detection of micrometastatic cells in bone marrow of uveal melanoma patients: A paradox","volume":"93","author":"Eide","year":"2015","journal-title":"Acta Ophthalmol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1002\/ijc.26037","article-title":"Expression of epithelial\u2013mesenchymal transition-inducing transcription factors in primary breast cancer: The effect of neoadjuvant therapy","volume":"130","author":"Mego","year":"2012","journal-title":"Int. J. Cancer"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.21873\/anticanres.13290","article-title":"Circulating Tumor Cells With Epithelial-to-mesenchymal Transition Phenotypes Associated With Inferior Outcomes in Primary Breast Cancer","volume":"39","author":"Mego","year":"2019","journal-title":"Anticancer Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"3934","DOI":"10.1158\/1078-0432.CCR-12-0309","article-title":"Pyrophosphorolysis-activated polymerization detects circulating tumor DNA in metastatic uveal melanoma","volume":"18","author":"Madic","year":"2012","journal-title":"Clin. Cancer Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1002\/cam4.61","article-title":"Ultradeep sequencing detects GNAQ and GNA11 mutations in cell-free DNA from plasma of patients with uveal melanoma","volume":"2","author":"Metz","year":"2013","journal-title":"Cancer Med."},{"key":"ref_63","first-page":"1","article-title":"ZEB1 regulates multiple oncogenic components involved in uveal melanoma progression","volume":"7","author":"Chen","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-019-52841-y","article-title":"Prospective validation in epithelial tumors of a gene expression predictor of liver metastasis derived from uveal melanoma","volume":"9","author":"Tsantoulis","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.jdermsci.2018.06.011","article-title":"miR-128 targets the CC chemokine ligand 18 gene (CCL18) in cutaneous malignant melanoma progression","volume":"91","author":"Song","year":"2018","journal-title":"J. Dermatol. Sci."},{"key":"ref_66","unstructured":"Hou, F., Huang, Q.-M., Hu, D.-N., Jonas, J.B., and Wei, W.-B. (2016). Immune oppression array elucidating immune escape and survival mechanisms in uveal melanoma. Int. J. Ophthalmol., 9."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"419","DOI":"10.2174\/156652410791316977","article-title":"CSPG4 in cancer: Multiple roles","volume":"10","author":"Wang","year":"2010","journal-title":"Curr. Mol. Med."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1136\/bjo.87.5.629","article-title":"Human uveal melanoma expresses NG2 immunoreactivity","volume":"87","author":"Li","year":"2003","journal-title":"Br. J. Ophthalmol."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Ilieva, K.M., Cheung, A., Mele, S., Chiaruttini, G., Crescioli, S., Griffin, M., Nakamura, M., Spicer, J.F., Tsoka, S., and Lacy, K.E. (2018). Chondroitin sulfate proteoglycan 4 and its potential as an antibody immunotherapy target across different tumor types. Front. Immunol., 8.","DOI":"10.3389\/fimmu.2017.01911"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1002\/path.4673","article-title":"Endo180 at the cutting edge of bone cancer treatment and beyond","volume":"238","author":"Sturge","year":"2016","journal-title":"J. Pathol."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Pan, B., Ye, Y., Liu, H., Zhen, J., Zhou, H., Li, Y., Qu, L., Wu, Y., Zeng, C., and Zhong, W. (2018). URG11 Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion. Biomed. Res. Int., 2018.","DOI":"10.1155\/2018\/4060728"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"197","DOI":"10.3727\/096504016X14648701447850","article-title":"Knockdown of downregulated Gene 11 (URG11) Inhibits Proliferation, Invasion, and \u03b2-Catenin Expression in Non-Small Cell Lung Cancer Cells","volume":"24","author":"Liu","year":"2016","journal-title":"Oncol. Res."},{"key":"ref_73","first-page":"1523","article-title":"Increased expression URG11 in hepatocellular carcinoma tissues promotes the growth of hepatocellular carcinoma cells","volume":"31","author":"Xie","year":"2015","journal-title":"Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.ydbio.2014.12.023","article-title":"Connexins in migration during development and cancer","volume":"401","author":"Kotini","year":"2015","journal-title":"Dev. Biol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.cell.2016.06.028","article-title":"EMT: 2016","volume":"166","author":"Nieto","year":"2016","journal-title":"Cell"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.immuni.2011.12.013","article-title":"Rarity of human T helper 17 cells is due to retinoic acid orphan receptor-dependent mechanisms that limit their expansion","volume":"36","author":"Santarlasci","year":"2012","journal-title":"Immunity"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"7205","DOI":"10.1158\/0008-5472.CAN-04-1750","article-title":"Gene expression profiling in uveal melanoma reveals two molecular classes and predicts metastatic death","volume":"64","author":"Onken","year":"2004","journal-title":"Cancer Res"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.molcel.2006.03.010","article-title":"Phosphorylation of SATB1, a global gene regulator, acts as a molecular switch regulating its transcriptional activity in vivo","volume":"22","author":"Kumar","year":"2006","journal-title":"Mol. 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