{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:51:26Z","timestamp":1761598286865,"version":"build-2065373602"},"reference-count":47,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2020,12,20]],"date-time":"2020-12-20T00:00:00Z","timestamp":1608422400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2020R1A6A1A03044977","2018008274"],"award-info":[{"award-number":["2020R1A6A1A03044977","2018008274"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In this study, hydrogen generation powder samples were prepared using zinc carbonate as a precursor, at a temperature varying from 400 to 700 \u00b0C in H2 atmosphere, and were characterized in terms of antioxidant activity. The concentration of dissolved hydrogen obtained by the powder samples was measured using a dissolved hydrogen meter as a function of time. In addition, the antioxidant activity of the samples was evaluated based on the Oyaizu\u2019s method, removal rate of \u00b7OH radicals, and ferric reducing antioxidant power. Finally, the hydrogen mask pack was fabricated using the hydrogen generation powder sample and gel-type emulsion. In the clinical test on the mask pack, the effect of the mask on skin aging was characterized and compared to that of a commercial sample. The skin densities of the participants in the experimental group and the control group increased by 18.41% and 9.93% after 4 weeks, respectively. The improved skin density of the participants who used the hydrogen mask pack in the experimental group, might be attributed to the recovery effect of the hydrogen molecule in the mask pack on the denatured thick skin layer.<\/jats:p>","DOI":"10.3390\/ijms21249731","type":"journal-article","created":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T01:01:08Z","timestamp":1608512468000},"page":"9731","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antioxidant Activity of Hydrogen Water Mask Pack Composed of Gel-Type Emulsion and Hydrogen Generation Powder"],"prefix":"10.3390","volume":"21","author":[{"given":"Hye-Jin","family":"Kwon","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, Soongsil University, Seoul 06978, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang-Beom","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Soongsil University, Seoul 06978, Korea"},{"name":"BoyazEnergy, 165 Gasandigital 2-ro, Geumcheon-gu, Seoul 08504, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung-Won","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Soongsil University, Seoul 06978, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"757","DOI":"10.2147\/CIA.S158513","article-title":"Oxidative stress, aging, and diseases","volume":"13","author":"Ilaria","year":"2018","journal-title":"Clin. Interv. Aging"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1111\/j.1532-5415.2010.02979.x","article-title":"Waist circumference but not body mass index predicts long-term mortality in elderly subjects with chronic heart failure","volume":"58","author":"Gianluca","year":"2010","journal-title":"J. Am. Geriatr. Soc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1097\/WOX.0b013e3182439613","article-title":"Oxidative stress and antioxidant defense","volume":"5","author":"Birben","year":"2012","journal-title":"World Allergy Organ. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.ejmech.2015.04.040","article-title":"The role of antioxidants in the chemistry of oxidative stress: A review","volume":"97","author":"Pisoschi","year":"2015","journal-title":"Eur. J. Med. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2076","DOI":"10.3390\/molecules24112076","article-title":"A New Approach for the Prevention and Treatment of Cardiovascular Disorders. Molecular Hydrogen Significantly Reduces the Eects of Oxidative Stress","volume":"24","author":"Tyler","year":"2019","journal-title":"Molecules"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1893","DOI":"10.1177\/147323001003800602","article-title":"Hydrogen as a Selective Antioxidant: Review of Clinical and Experimental Studies","volume":"38","author":"Hong","year":"2010","journal-title":"J. Int. Med. Res."},{"key":"ref_7","first-page":"158","article-title":"Medicalization, Markets and Consumers","volume":"45","author":"Peter","year":"2004","journal-title":"J. Health Soc. Behav."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Linda, S., Matias, R., Martin, N.F., and Gunnar, J. (2018). Percutaneous absorption of thirty-eight organic solvents in vitro using pig skin. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0205458"},{"key":"ref_9","first-page":"1","article-title":"Skin care and rejuvenation by cosmeceutical facial mask","volume":"17","author":"Mohammad","year":"2018","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1294\/jes.24.1","article-title":"Effect of treadmill exercise and hydrogen-rich water intake on serum oxidative and anti-oxidative metabolites in serum of thorough bred horses","volume":"24","author":"Tsubone","year":"2013","journal-title":"J. Equine Sci."},{"key":"ref_11","first-page":"66","article-title":"Hydrogen reduction of zinc and iron oxides containing mixtures","volume":"45B","author":"Motta","year":"2014","journal-title":"Metall. Mater. Trans. B"},{"key":"ref_12","first-page":"1","article-title":"Iron (FeII) Chelation, Ferric Reducing Antioxidant Power, and Immune Modulating Potential of Arisaema jacquemontii (Himalayan Cobra Lily)","volume":"2014","author":"Rasleen","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1093\/geronj\/11.3.298","article-title":"Aging\u2014A theory based on free-radical and radiation-chemistry","volume":"11","author":"Harman","year":"1956","journal-title":"J. Gerontol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1111\/j.1365-2133.2003.05618.x","article-title":"Aspartic acid racemization: Evidence for marked longevity of elastin in human skin","volume":"149","author":"Laumeier","year":"2003","journal-title":"Br. J. Dermatol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.maturitas.2011.04.011","article-title":"Molecular aspects of skin ageing","volume":"69","author":"Naylor","year":"2011","journal-title":"Maturitas"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1016\/S0047-6374(01)00425-0","article-title":"Molecular mechanisms of skin ageing","volume":"123","author":"Jenkins","year":"2002","journal-title":"Mech. Ageing Dev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1309\/LM93W7KTFNPZIXRR","article-title":"Ferric reducing capacity versus ferric reducing antioxidant power for measuring total antioxidant capacity","volume":"44","author":"Christopher","year":"2013","journal-title":"Lab. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pharmthera.2014.04.006","article-title":"Molecular hydrogen as a preventive and therapeutic medical gas: Initiation, development and potential of hydrogen medicine","volume":"144","author":"Ohta","year":"2014","journal-title":"Pharmacol. Ther."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"326","DOI":"10.21037\/atm.2017.06.27","article-title":"The role of reactive oxygen species in the pathophysiology of cardiovascular diseases and the clinical significance of myocardial redox","volume":"5","author":"Moris","year":"2017","journal-title":"Ann. Transl. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/nm1577","article-title":"Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals","volume":"13","author":"Ikuroh","year":"2007","journal-title":"Nat. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1126\/science.1166304","article-title":"Hyperbaric Hydrogen Therapy: A possible Treatment for Cancer","volume":"190","author":"Dole","year":"1975","journal-title":"Science"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"8402","DOI":"10.1073\/pnas.96.15.8402","article-title":"A Novel Function for Transglutaminase 1: Attachment of Long-Chain \u03c9 -Hydroxyceramides to Involucrin by Ester Bond Formation","volume":"96","author":"Lyuben","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.jphotobiol.2014.12.025","article-title":"A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins","volume":"144","author":"Alessandro","year":"2015","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/978-94-007-7893-1_3","article-title":"Basic components of connective tissues and extracellular matrix: Elastin, fibrillin, fibulins, fibrinogen, fibronectin, laminin, tenascins and thrombospondins","volume":"802","author":"Halper","year":"2014","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.jdermsci.2010.09.001","article-title":"Neutrophil elastase contributes to extracellular matrix damage induced by chronic lowdose UV irradiation in a hairless mouse photoaging model","volume":"60","author":"Takeuchi","year":"2010","journal-title":"J. Dermatol. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1111\/bjd.13436","article-title":"Propionibacterium species and follicular keratinocyte activation in acneic and normal skin","volume":"172","author":"Jahns","year":"2015","journal-title":"Br. J. Dermatol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1159\/000060656","article-title":"UV-induced oxidative stress and photoaging","volume":"29","author":"Wenk","year":"2001","journal-title":"Curr. Probl. Dermatol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1111\/j.1751-1097.1997.tb08571.x","article-title":"Interactions between vitamin E homologues and ascorbate free radicals in murine skin homogenates irradiated with ultraviolet light","volume":"65","author":"Kitazawa","year":"1997","journal-title":"Photochem. Photobiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Shin, M.H., Park, R., Nojima, H., Kim, H.C., Kim, Y.K., and Chung, J.H. (2013). Atomic Hydrogen Surrounded by Water Molecules, H(H2O)m, Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0061696"},{"key":"ref_30","first-page":"1247","article-title":"UV-induced reactive oxygen species in photocarcinogenesis and photoaging","volume":"378","author":"Wlaschek","year":"1997","journal-title":"Biol. Chem."},{"key":"ref_31","first-page":"12","article-title":"Collagen glycation triggers the formation of aged skin in vitro","volume":"17","author":"Pageon","year":"2007","journal-title":"Eur. J. Dermatol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1016\/j.jss.2018.03.024","article-title":"Hydrogen-rich water attenuates oxidative stress in rats with traumatic brain injury via Nrf2 pathway","volume":"228","author":"Yuan","year":"2018","journal-title":"J. Surg. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1139\/cjpp-2018-0604","article-title":"Molecular hydrogen: Potential in mitigating oxidative-stress-induced radiation injury","volume":"97","author":"Kura","year":"2019","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0033-0620(84)90003-3","article-title":"Radiation-induced heart disease: An update","volume":"27","author":"Stewart","year":"1984","journal-title":"Prog. Cardiovasc. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"35766","DOI":"10.1074\/jbc.M202959200","article-title":"Interferons inhibit tumor necrosis factor-alpha-mediated matrix metalloproteinase-9 activation via interferon regulatory factor-1 binding competition with NF-kappa B","volume":"277","author":"Sanceau","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1111\/1523-1747.ep12532770","article-title":"Age-related Changes in the Mechanical Properties of Human Skin","volume":"73","author":"Daly","year":"1979","journal-title":"J. Investig. Dermatol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1083\/jcb.201102095","article-title":"Signal transduction by reactive oxygen species","volume":"194","author":"Finkel","year":"2011","journal-title":"J. Cell Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1161\/CIRCRESAHA.109.206607","article-title":"Metabolic remodeling induced by mitochondrial aldehyde stress stimulates tolerance to oxidative stress in the heart","volume":"105","author":"Endo","year":"2009","journal-title":"Circ. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-230X-14-12","article-title":"Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release","volume":"14","author":"Liu","year":"2014","journal-title":"BMC Gastroenterol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.2174\/1389450111009011517","article-title":"Targeting Heme Oxygenase-1 for Neuroprotection and Neuroinflammation in Neurodegenerative Diseases","volume":"11","author":"Jazwa","year":"2010","journal-title":"Curr. Drug Targets"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"13291","DOI":"10.1074\/jbc.R900010200","article-title":"The Nrf2-antioxidant response element signaling pathway and its activation by oxidative stress","volume":"284","author":"Nguyen","year":"2009","journal-title":"J. Biol. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"307","DOI":"10.5264\/eiyogakuzashi.44.307","article-title":"Studies on products of browning reaction prepared from glucosamine","volume":"44","author":"Oyaizu","year":"1986","journal-title":"Jpn. J. Nutr."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1271\/bbb.61.118","article-title":"Hydroxyl Radical-scavenging Effects of Spices and Scavengers from Brown Mustard (Brassica nigra)","volume":"61","author":"Chung","year":"1997","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The ferric reducing ability of plasma (FRAP) as a measure of \u201cantioxidant power\u201d: The FRAP assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref_45","first-page":"31","article-title":"Considerations Regarding the Importance of In Vivo Data for the Development of an Alternative to Conventional Skin Irritation Testing","volume":"11","author":"Kojima","year":"2005","journal-title":"Altern. Anim. Test. Exp."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1111\/exd.13743","article-title":"Noninvasive analysis and minimally invasive in vivo experimental challenges of the skin barrier","volume":"27","author":"Piet","year":"2018","journal-title":"Exp. Dermatol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1111\/j.1600-0536.2007.01138.x","article-title":"Biophysical parameters of skin: Map of human face, regional, and age-related differences","volume":"57","author":"Marrakchi","year":"2007","journal-title":"Contact Dermat."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/24\/9731\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:47:36Z","timestamp":1760179656000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/21\/24\/9731"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,20]]},"references-count":47,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["ijms21249731"],"URL":"https:\/\/doi.org\/10.3390\/ijms21249731","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2020,12,20]]}}}