{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:10:18Z","timestamp":1790737818316,"version":"4.1.0"},"reference-count":48,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2020,12,20]],"date-time":"2020-12-20T00:00:00Z","timestamp":1608422400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003822","name":"Joint Institute for Nuclear Research","doi-asserted-by":"publisher","award":["Themes 02-1-1107-2011\/2021, 04-5-1131-2017\/2021 and 04-4-1121-2015\/2020"],"award-info":[{"award-number":["Themes 02-1-1107-2011\/2021, 04-5-1131-2017\/2021 and 04-4-1121-2015\/2020"]}],"id":[{"id":"10.13039\/501100003822","id-type":"DOI","asserted-by":"publisher"}]},{"name":"RO-JINR","award":["Themes 02-1-1107-2011\/2021, 04-5-1131-2017\/2021 and 04-4-1121-2015\/2020"],"award-info":[{"award-number":["Themes 02-1-1107-2011\/2021, 04-5-1131-2017\/2021 and 04-4-1121-2015\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In the last few years, a great amount of attention has been given to nanoparticles research due to their physicochemical properties that allow their use in analytical instruments or in promising imaging applications on biological systems. The use of ferrihydrite nanoparticles (Fh-NPs) in practical applications implies a particular control of their magnetic properties, stability, biocompatibility, interaction with the surface of the target, and low toxicity. In this study, the formation and organization of human serum albumin (HSA) molecules around the simple Fh-NPs and Fh-NPs doped with Co and Cu were examined by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) in terms of morphology and particle size. The topology of all Fh-NPs shows an organized area of HSA around each type of Fh-NP. Molecular docking studies were used in order to determine the probable location of the ferrihydrite in the HSA structure. The thermal stability of these nanohybrids was further investigated by fluorimetry, using 214-Trp residue from HSA as a spectral sensor. The denaturation temperature (Tm) was determined, and stabilization of the HSA structure in the presence of Fh-NPs was discussed. This study could be a starting point for the development of different applications targeting the structure and stability of Fh-NPs complexes with proteins.<\/jats:p>","DOI":"10.3390\/ijms21249734","type":"journal-article","created":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T01:01:08Z","timestamp":1608512468000},"page":"9734","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Exploring the Conformation and Thermal Stability of Human Serum Albumin Corona of Ferrihydrite Nanoparticles"],"prefix":"10.3390","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3662-9268","authenticated-orcid":false,"given":"Claudia G.","family":"Chilom","sequence":"first","affiliation":[{"name":"Faculty of Physics, University of Bucharest, Str Atomi\u0219tilor 405, CP MG 11, RO-077125 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"B\u0103lan","sequence":"additional","affiliation":[{"name":"Faculty of Physics, University of Bucharest, Str Atomi\u0219tilor 405, CP MG 11, RO-077125 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicoleta","family":"Sandu","sequence":"additional","affiliation":[{"name":"Faculty of Physics, University of Bucharest, Str Atomi\u0219tilor 405, CP MG 11, RO-077125 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0900-2654","authenticated-orcid":false,"given":"Maria","family":"B\u0103l\u0103\u015foiu","sequence":"additional","affiliation":[{"name":"Joint Institute for Nuclear Research, Joliot-Curie No.6, 141980 Dubna, Russia"},{"name":"Moscow Institute of Physics and Technology, Institutskiy Per. 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