{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:55:26Z","timestamp":1790398526838,"version":"4.1.0"},"reference-count":29,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T00:00:00Z","timestamp":1608681600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Economy and Competitivity of Spain and Fondo Europeo de Desarrollo Regional FEDER","award":["BFU2014-57736-P, AGL2014-58883-R, SAF2017-88457-R, AGL2017-85270-R"],"award-info":[{"award-number":["BFU2014-57736-P, AGL2014-58883-R, SAF2017-88457-R, AGL2017-85270-R"]}]},{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda","doi-asserted-by":"publisher","award":["CTS235, CTS164"],"award-info":[{"award-number":["CTS235, CTS164"]}],"id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CIBERehd"],"award-info":[{"award-number":["CIBERehd"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The liver expresses tissue-nonspecific alkaline phosphatase (TNAP), which may participate in the defense against bacterial components, in cell regulation as part of the purinome or in bile secretion, among other roles. We aimed to study the role of TNAP in the development of hepatosteatosis. TNAP+\/\u2212 haplodeficient and wild type (WT) mice were fed a control diet (containing 10% fat w\/w) or the same diet deficient in methionine and choline (MCD diet). The MCD diet induced substantial weight loss together with hepatic steatosis and increased alanine aminotransferase (ALT) plasma levels, but no differences in IL-6, TNF, insulin or resistin. There were no substantial differences between TNAP+\/\u2212 and WT mice fed the MCD diet. In turn, TNAP+\/\u2212 mice receiving the control diet presented hepatic steatosis with alterations in metabolic parameters very similar to those induced by the MCD diet. Nevertheless, no weight loss, increased ALT plasma levels or hypoglycemia were observed. These mice also presented increased levels of liver TNF and systemic resistin and glucagon compared to WT mice. The phenotype of TNAP+\/\u2212 mice fed a standard diet was normal. In conclusion, TNAP haplodeficiency induces steatosis comparable to that produced by a MCD diet when fed a control diet.<\/jats:p>","DOI":"10.3390\/ijms22010051","type":"journal-article","created":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T08:38:43Z","timestamp":1608712723000},"page":"51","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Deficiency in Tissue Non-Specific Alkaline Phosphatase Leads to Steatohepatitis in Mice Fed a High Fat Diet Similar to That Produced by a Methionine and Choline Deficient Diet"],"prefix":"10.3390","volume":"22","author":[{"given":"Reyes","family":"G\u00e1mez-Belmonte","sequence":"first","affiliation":[{"name":"Department of Pharmacology, CIBERehd, School of Pharmacy, Instituto de Investigaci\u00f3n Biosanitaria ibs.GRANADA, University of Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8956-773X","authenticated-orcid":false,"given":"Mireia","family":"Tena-Garitaonaindia","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology 2, CIBERehd, School of Pharmacy, Instituto de Investigaci\u00f3n Biosanitaria ibs.GRANADA, Instituto de Nutrici\u00f3n y Tecnolog\u00eda de los Alimentos Jos\u00e9 Mataix, University of Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Hern\u00e1ndez-Chirlaque","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology 2, CIBERehd, School of Pharmacy, Instituto de Investigaci\u00f3n Biosanitaria ibs.GRANADA, Instituto de Nutrici\u00f3n y Tecnolog\u00eda de los Alimentos Jos\u00e9 Mataix, University of Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3815-1587","authenticated-orcid":false,"given":"Samir","family":"C\u00f3rdova","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology 2, CIBERehd, School of Pharmacy, Instituto de Investigaci\u00f3n Biosanitaria ibs.GRANADA, Instituto de Nutrici\u00f3n y Tecnolog\u00eda de los Alimentos Jos\u00e9 Mataix, University of Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1048-5905","authenticated-orcid":false,"given":"Diego","family":"Ceacero-Heras","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology 2, CIBERehd, School of Pharmacy, Instituto de Investigaci\u00f3n Biosanitaria ibs.GRANADA, Instituto de Nutrici\u00f3n y Tecnolog\u00eda de los Alimentos Jos\u00e9 Mataix, University of Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4516-5824","authenticated-orcid":false,"given":"Ferm\u00edn S\u00e1nchez","family":"de Medina","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, CIBERehd, School of Pharmacy, Instituto de Investigaci\u00f3n Biosanitaria ibs.GRANADA, University of Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8291-3468","authenticated-orcid":false,"given":"Olga","family":"Mart\u00ednez-Augustin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology 2, CIBERehd, School of Pharmacy, Instituto de Investigaci\u00f3n Biosanitaria ibs.GRANADA, Instituto de Nutrici\u00f3n y Tecnolog\u00eda de los Alimentos Jos\u00e9 Mataix, University of Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"206","DOI":"10.2741\/4892","article-title":"Current treatment paradigms and emerging therapies for NAFLD\/NASH","volume":"26","author":"Raza","year":"2021","journal-title":"Front. 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