{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T06:52:43Z","timestamp":1781592763586,"version":"3.54.5"},"reference-count":55,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T00:00:00Z","timestamp":1608768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Sorbus commixta is a valuable hardwood plant with a high economical value for its medicinal and ornamental qualities. The aim of this work was to investigate the effects of the iron (Fe) source and medium pH on the growth and development of S. commixta in vitro. The Fe sources used, including non-chelated iron sulfate (FeSO4), iron ethylenediaminetetraacetic acid (Fe-EDTA), and iron diethylenetriaminepentaacetic acid (Fe-DTPA), were supplemented to the Multipurpose medium with a final Fe concentration of 2.78 mg\u00b7L\u22121. The medium without any supplementary Fe was used as the control. The pH of the agar-solidified medium was adjusted to either 4.70, 5.70, or 6.70. The experiment was conducted in a culture room for six weeks with 25 \u00b0C day and night temperatures, and a 16-h photoperiod with a light intensity of 50 mmol\u00b7m\u22122\u00b7s\u22121 photosynthetic photon flux density (PPFD). Both the Fe source and pH affected the growth and development of the micropropagated plants in vitro. The leaves were greener in the pH 4.70 and 5.70 treatments. The tissue Fe content decreased with the increase of the medium pH. The leaf chlorophyll content was similar between plants treated with FeSO4 and those with Fe-EDTA. The numbers of the shoots and roots of plantlets treated with FeSO4 were 2.5 and 2 times greater than those of the control, respectively. The fresh and dry weights of the shoot and the root were the greatest for plants treated with Fe-EDTA combined with pH 5.70. The calcium, magnesium, and manganese contents in the plantlets increased in the pH 5.70 treatments regardless of the Fe source. Supplementary Fe decreased the activity of ferric chelate reductase. Overall, although the plantlets absorbed more Fe at pH 4.70, Fe-EDTA combined with pH 5.70 was found to be the best for the growth and development of S. commixta in vitro.<\/jats:p>","DOI":"10.3390\/ijms22010133","type":"journal-article","created":{"date-parts":[[2020,12,24]],"date-time":"2020-12-24T22:56:45Z","timestamp":1608850605000},"page":"133","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Effect of Iron Source and Medium pH on Growth and Development of Sorbus commixta In Vitro"],"prefix":"10.3390","volume":"22","author":[{"given":"Jie","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Horticulture, Division of Applied Life Science (BK21 Program), Graduate School of Gyeongsang National University, Jinju 52828, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoo Gyeong","family":"Park","sequence":"additional","affiliation":[{"name":"Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Horticulture, Division of Applied Life Science (BK21 Program), Graduate School of Gyeongsang National University, Jinju 52828, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4146-7278","authenticated-orcid":false,"given":"Byoung Ryong","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Horticulture, Division of Applied Life Science (BK21 Program), Graduate School of Gyeongsang National University, Jinju 52828, Korea"},{"name":"Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea"},{"name":"Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.5352\/JLS.2007.17.12.1610","article-title":"Phylogenetic study of genus Sorbus in Korea by internal transcribed spacer sequence (ITS)","volume":"17","author":"Huh","year":"2007","journal-title":"J. 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