{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T17:32:51Z","timestamp":1778693571804,"version":"3.51.4"},"reference-count":26,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,8]],"date-time":"2021-01-08T00:00:00Z","timestamp":1610064000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Regional Fund and Croatian Ministry of Science Infrastructural Project grant","award":["KK.01.1.1.02.0015"],"award-info":[{"award-number":["KK.01.1.1.02.0015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The aim of this study was to develop and evaluate matrix assisted LASER desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry imaging (MSI) of blood smear. Integrated light microscope and MALDI IT-TOF mass spectrometer, together with a matrix sublimation device, were used for analysis of blood smears coming from healthy male donors. Different blood plasma removal, matrix deposition, and instrumental settings were evaluated using the negative and positive ionization modes while agreement between the light microscopy images and the lateral distributions of cellular marker compounds served as the MSI quality indicator. Red and white blood cells chemical composition was analyzed using the differential m\/z expression. Five seconds of exposure to ethanol followed by the 5 min of 9-aminoacridine or \u03b1-cyano-4-hydroxycinnamic acid deposition, together with two sets of instrumental settings, were selected for the MALDI TOF MSI experiments. Application of the thin and transparent matrix layers assured good correspondence between the LASER footprints and the preselected regions of interest. Cellular marker m\/z signals coincided well with the appropriate cells. A metabolite databases search using the differentially expressed m\/z produced hits which were consistent with the respective cell types. This study sets the foundations for application of blood smear MALDI TOF MSI in clinical diagnostics and research.<\/jats:p>","DOI":"10.3390\/ijms22020585","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T19:55:56Z","timestamp":1610308556000},"page":"585","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["MALDI TOF Mass Spectrometry Imaging of Blood Smear: Method Development and Evaluation"],"prefix":"10.3390","volume":"22","author":[{"given":"\u017deljko","family":"Debeljak","sequence":"first","affiliation":[{"name":"Clinical Institute of Laboratory Diagnostics, Osijek University Hospital, J. Huttlera 4, 31 000 Osijek, Croatia"},{"name":"Department of Pharmacology, Faculty of Medicine, JJ Strossmayer University of Osijek, J. Huttlera 4, 31 000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann-Christin","family":"Niehoff","sequence":"additional","affiliation":[{"name":"European Innovation Center, Shimadzu Europa GmbH, Albert-Hahn-Stra\u00dfe 6-10, 47269 Duisburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Bandjak","sequence":"additional","affiliation":[{"name":"Clinical Institute of Laboratory Diagnostics, Osijek University Hospital, J. Huttlera 4, 31 000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3792-4384","authenticated-orcid":false,"given":"Dario","family":"Mandi\u0107","sequence":"additional","affiliation":[{"name":"Clinical Institute of Laboratory Diagnostics, Osijek University Hospital, J. 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Huttlera 4, 31 000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Mr\u0111enovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Hematology, Internal Medicine Clinic, Osijek University Hospital, J. Huttlera 4, 31 000 Osijek, Croatia"},{"name":"Department of Internal Medicine, Family Medicine and History of Medicine, Faculty of Medicine Osijek, JJ Strossmayer University of Osijek, J. Huttlera 4, 31 000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Markovi\u0107","sequence":"additional","affiliation":[{"name":"Clinical Institute of Laboratory Diagnostics, Osijek University Hospital, J. Huttlera 4, 31 000 Osijek, Croatia"},{"name":"Department of Medical Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, JJ Strossmayer University of Osijek, J. Huttlera 4, 31 000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milorad","family":"Zjali\u0107","sequence":"additional","affiliation":[{"name":"Department of Medical Biology and Genetics, Faculty of Medicine, JJ Strossmayer University of Osijek, J. Huttlera 4, 31 000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vatroslav","family":"\u0160eri\u0107","sequence":"additional","affiliation":[{"name":"Clinical Institute of Laboratory Diagnostics, Osijek University Hospital, J. Huttlera 4, 31 000 Osijek, Croatia"},{"name":"Department of Medical Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, JJ Strossmayer University of Osijek, J. Huttlera 4, 31 000 Osijek, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,8]]},"reference":[{"key":"ref_1","unstructured":"McKenzie, S.B., and Williams, L. (2015). 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