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Although binge drinking is the most studied prenatal alcohol exposure pattern, other types of exposure, such as the Mediterranean, are common in specific geographic areas. In this study, we analyze the effects of prenatal alcohol exposure in binge and Mediterranean human drinking patterns on placenta and brain development in C57BL\/6J mice. We also assess the impact of prenatal treatment with the epigallocatechin-3-gallate antioxidant in both groups. Study experimental groups for Mediterranean or binge patterns: (1) control; (2) ethanol; (3) ethanol + epigallocatechin-3-gallate. Brain and placental tissue were collected on gestational Day 19. The molecular pathways studied were fetal and placental growth, placental angiogenesis (VEGF-A, PLGF, VEGF-R), oxidative stress (Nrf2), and neurodevelopmental processes including maturation (NeuN, DCX), differentiation (GFAP) and neural plasticity (BDNF). Prenatal alcohol exposure resulted in fetal growth restriction and produced imbalances of placental angiogenic factors. Moreover, prenatal alcohol exposure increased oxidative stress and caused significant alterations in neuronal maturation and astrocyte differentiation. Epigallocatechin-3-gallate therapy ameliorated fetal growth restriction, attenuated alcohol-induced changes in placental angiogenic factors, and partially rescued neuronal nuclear antigen (NeuN), (doublecortin) DCX, and (glial fibrillary acidic protein) GFAP levels. Any alcohol consumption (Mediterranean or binge) during pregnancy may generate a fetal alcohol spectrum disorder phenotype and the consequences may be partially attenuated by a prenatal treatment with epigallocatechin-3-gallate.<\/jats:p>","DOI":"10.3390\/ijms22020715","type":"journal-article","created":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T11:52:32Z","timestamp":1610538752000},"page":"715","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Epigallocatechin Gallate Ameliorates the Effects of Prenatal Alcohol Exposure in a Fetal Alcohol Spectrum Disorder-Like Mouse Model"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9263-7222","authenticated-orcid":false,"given":"Laura","family":"Almeida-Toledano","sequence":"first","affiliation":[{"name":"BCNatal-Barcelona Center for Maternal Fetal and Neonatal Medicine, Hospital Sant Joan de D\u00e9u and Hospital Cl\u00ednic, 08950 Esplugues de Llobregat, Spain"},{"name":"Institut de Recerca Sant Joan de D\u00e9u, 08950 Esplugues de Llobregat, Spain"},{"name":"Maternal and Child Health and Development Network II (SAMID II), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7183-8706","authenticated-orcid":false,"given":"Vicente","family":"Andreu-Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Grup de Recerca Infancia i Entorn (GRIE), Institut d\u2019investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain"},{"name":"Valencian International University (VIU), 46002 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Aras-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Maternal and Child Health and Development Network II (SAMID II), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"},{"name":"Congenital Malformations Lab, Institute of Medicine and Molecular Genetic (INGEMM), Institute for Health Research of La Paz University Hospital (IdiPAZ), 28046 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5708-4362","authenticated-orcid":false,"given":"\u00d3scar","family":"Garc\u00eda-Algar","sequence":"additional","affiliation":[{"name":"BCNatal-Barcelona Center for Maternal Fetal and Neonatal Medicine, Hospital Sant Joan de D\u00e9u and Hospital Cl\u00ednic, 08950 Esplugues de Llobregat, Spain"},{"name":"Maternal and Child Health and Development Network II (SAMID II), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"},{"name":"Grup de Recerca Infancia i Entorn (GRIE), Institut d\u2019investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain"},{"name":"Department of Neonatology, Hospital Cl\u00ednic-Maternitat, ICGON, IDIBAPS, BCNatal, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leopoldo","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Maternal and Child Health and Development Network II (SAMID II), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"},{"name":"Congenital Malformations Lab, Institute of Medicine and Molecular Genetic (INGEMM), Institute for Health Research of La Paz University Hospital (IdiPAZ), 28046 Madrid, Spain"},{"name":"Department of Pediatric Surgery, Hospital Universitario La Paz, 28046 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Dolores","family":"G\u00f3mez-Roig","sequence":"additional","affiliation":[{"name":"BCNatal-Barcelona Center for Maternal Fetal and Neonatal Medicine, Hospital Sant Joan de D\u00e9u and Hospital Cl\u00ednic, 08950 Esplugues de Llobregat, Spain"},{"name":"Institut de Recerca Sant Joan de D\u00e9u, 08950 Esplugues de Llobregat, Spain"},{"name":"Maternal and Child Health and Development Network II (SAMID II), Instituto de Salud Carlos III (ISCIII), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e20154256","DOI":"10.1542\/peds.2015-4256","article-title":"Updated Clinical Guidelines for Diagnosing Fetal Alcohol Spectrum Disorders","volume":"138","author":"Hoyme","year":"2016","journal-title":"Pediatrics"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1111\/acer.13022","article-title":"Alcohol, Methamphetamine, and Marijuana Exposure Have Distinct Effects on the Human Placenta","volume":"40","author":"Carter","year":"2016","journal-title":"Alcohol Clin. 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