{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T10:41:34Z","timestamp":1785148894413,"version":"3.55.0"},"reference-count":61,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T00:00:00Z","timestamp":1610668800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["EY-022408"],"award-info":[{"award-number":["EY-022408"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Mesenchymal stem cells (MSCs) are a promising therapy to improve vascular repair, yet their role in ischemic retinopathy is not fully understood. The aim of this study is to investigate the impact of modulating the neurotrophin receptor; p75NTR on the vascular protection of MSCs in an acute model of retinal ischemia\/reperfusion (I\/R). Wild type (WT) and p75NTR-\/- mice were subjected to I\/R injury by increasing intra-ocular pressure to 120 mmHg for 45 min, followed by perfusion. Murine GFP-labeled MSCs (100,000 cells\/eye) were injected intravitreally 2 days post-I\/R and vascular homing was assessed 1 week later. Acellular capillaries were counted using trypsin digest 10-days post-I\/R. In vitro, MSC-p75NTR was modulated either genetically using siRNA or pharmacologically using the p75NTR modulator; LM11A-31, and conditioned media were co-cultured with human retinal endothelial cells (HREs) to examine the angiogenic response. Finally, visual function in mice undergoing retinal I\/R and receiving LM11A-31 was assessed by visual-clue water-maze test. I\/R significantly increased the number of acellular capillaries (3.2-Fold) in WT retinas, which was partially ameliorated in p75NTR-\/- retinas. GFP-MSCs were successfully incorporated and engrafted into retinal vasculature 1 week post injection and normalized the number of acellular capillaries in p75NTR-\/- retinas, yet ischemic WT retinas maintained a 2-Fold increase. Silencing p75NTR on GFP-MSCs coincided with a higher number of cells homing to the ischemic WT retinal vasculature and normalized the number of acellular capillaries when compared to ischemic WT retinas receiving scrambled-GFP-MSCs. In vitro, silencing p75NTR-MSCs enhanced their secretome, as evidenced by significant increases in SDF-1, VEGF and NGF release in MSCs conditioned medium; improved paracrine angiogenic response in HREs, where HREs showed enhanced migration (1.4-Fold) and tube formation (2-Fold) compared to controls. In parallel, modulating MSCs-p75NTR using LM11A-31 resulted in a similar improvement in MSCs secretome and the enhanced paracrine angiogenic potential of HREs. Further, intervention with LM11A-31 significantly mitigated the decline in visual acuity post retinal I\/R injury. In conclusion, p75NTR modulation can potentiate the therapeutic potential of MSCs to harness vascular repair in ischemic retinopathy diseases.<\/jats:p>","DOI":"10.3390\/ijms22020829","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T12:44:05Z","timestamp":1610714645000},"page":"829","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Modulation of p75NTR on Mesenchymal Stem Cells Increases Their Vascular Protection in Retinal Ischemia-Reperfusion Mouse Model"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6462-8169","authenticated-orcid":false,"given":"Sally L.","family":"Elshaer","sequence":"first","affiliation":[{"name":"Augusta Biomedical Research Corporation, Charlie Norwood VA Medical Center, Augusta, GA 30901, USA"},{"name":"Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9130-8478","authenticated-orcid":false,"given":"Hang-soo","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Illinois at Chicago, Chicago, IL 60612, USA"},{"name":"Department of Obstetrics and Gynecology, University of Chicago, Chicago, IL 60637, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Pearson","sequence":"additional","affiliation":[{"name":"Augusta Biomedical Research Corporation, Charlie Norwood VA Medical Center, Augusta, GA 30901, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William D.","family":"Hill","sequence":"additional","affiliation":[{"name":"Augusta Biomedical Research Corporation, Charlie Norwood VA Medical Center, Augusta, GA 30901, USA"},{"name":"Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC 29403, USA"},{"name":"Ralph H. Johnson Veterans Affairs Medical Center, Charleston, SC 29403, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank M.","family":"Longo","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurological Sciences, Stanford University, Palo Alto, CA 94304, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4386-1989","authenticated-orcid":false,"given":"Azza B.","family":"El-Remessy","sequence":"additional","affiliation":[{"name":"Augusta Biomedical Research Corporation, Charlie Norwood VA Medical Center, Augusta, GA 30901, USA"},{"name":"Department of Surgery, University of Illinois at Chicago, Chicago, IL 60612, USA"},{"name":"Department of the Pharmacy, Doctors Hospital of Augusta, Augusta, GA 30909, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1016\/j.preteyeres.2003.08.001","article-title":"Retinal and choroidal angiogenesis: Pathophysiology and strategies for inhibition","volume":"22","author":"Das","year":"2003","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_2","first-page":"224","article-title":"Oxygen and blood flow: Players in the pathogenesis of glaucoma","volume":"14","author":"Mozaffarieh","year":"2008","journal-title":"Mol. Vis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"135","DOI":"10.4103\/0974-9233.154386","article-title":"Molecular mechanisms of diabetic retinopathy: Potential therapeutic targets","volume":"22","author":"Coucha","year":"2015","journal-title":"Middle East. Afr J. Ophthalmol."},{"key":"ref_4","first-page":"36","article-title":"Pan-American Collaborative Retina Study, G. Intravitreal Bevacizumab in Diabetic Retinopathy. Recommendations from the Pan-American Collaborative Retina Study Group (PACORES): The 2016 Knobloch Lecture","volume":"7","author":"Arevalo","year":"2018","journal-title":"Asia Pac. J. Ophthalmol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ding, S.L.S., Kumar, S., and Mok, P.L. (2017). Cellular Reparative Mechanisms of Mesenchymal Stem Cells for Retinal Diseases. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18081406"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1186\/s13287-017-0742-8","article-title":"Immunoprivileged no more: Measuring the immunogenicity of allogeneic adult mesenchymal stem cells","volume":"8","author":"Berglund","year":"2017","journal-title":"Stem Cell Res. Ther."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1016\/S0301-472X(03)00110-3","article-title":"HLA expression and immunologic properties of differentiated and undifferentiated mesenchymal stem cells","volume":"31","author":"Tammik","year":"2003","journal-title":"Exp. Hematol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Lalu, M.M., McIntyre, L., Pugliese, C., Fergusson, D., Winston, B.W., Marshall, J.C., Granton, J., Stewart, D.J., and Canadian Critical Care Trials, G. (2012). Safety of cell therapy with mesenchymal stromal cells (SafeCell): A systematic review and meta-analysis of clinical trials. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0047559"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.cytogfr.2009.10.002","article-title":"Mechanisms involved in the therapeutic properties of mesenchymal stem cells","volume":"20","author":"Fontes","year":"2009","journal-title":"Cytokine Growth Factor Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1186\/s13287-018-1059-y","article-title":"Adipose stem cells and their paracrine factors are therapeutic for early retinal complications of diabetes in the Ins2(Akita) mouse","volume":"9","author":"Elshaer","year":"2018","journal-title":"Stem Cell Res. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.3727\/096368911X623862","article-title":"Adipose stromal vascular fraction improves cardiac function in chronic myocardial infarction through differentiation and paracrine activity","volume":"21","author":"Mazo","year":"2012","journal-title":"Cell Transplant."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7742","DOI":"10.1523\/JNEUROSCI.23-21-07742.2003","article-title":"Differentiation of marrow stromal cells into photoreceptors in the rat eye","volume":"23","author":"Kicic","year":"2003","journal-title":"J. Neurosci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"107671","DOI":"10.1016\/j.exer.2019.05.011","article-title":"Mesenchymal stem cells provide paracrine neuroprotective resources that delay degeneration of co-cultured organotypic neuroretinal cultures","volume":"185","author":"Srivastava","year":"2019","journal-title":"Exp. Eye Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"321","DOI":"10.2217\/rme-2019-0022","article-title":"The use of mesenchymal stem cells for the treatment of progressive retinal diseases: A review","volume":"14","author":"Slugocka","year":"2019","journal-title":"Regen. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1080\/17469899.2017.1269602","article-title":"Implication of the neurotrophin receptor p75(NTR) in vascular diseases: Beyond the eye","volume":"12","author":"Elshaer","year":"2017","journal-title":"Expert Rev. Ophthalmol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2467","DOI":"10.1007\/s00018-014-1564-9","article-title":"Role of p75 neurotrophin receptor in stem cell biology: More than just a marker","volume":"71","author":"Tomellini","year":"2014","journal-title":"Cell Mol. Life Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.neures.2009.09.1711","article-title":"Adult rat mesenchymal stem cells differentiate into neuronal-like phenotype and express a variety of neuro-regulatory molecules in vitro","volume":"66","author":"Jiang","year":"2010","journal-title":"Neurosci. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1089\/scd.2010.0299","article-title":"CD271\/p75(NTR) inhibits the differentiation of mesenchymal stem cells into osteogenic, adipogenic, chondrogenic, and myogenic lineages","volume":"20","author":"Mikami","year":"2011","journal-title":"Stem Cells Dev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2329","DOI":"10.1007\/s00125-013-2998-6","article-title":"Modulation of p75(NTR) prevents diabetes- and proNGF-induced retinal inflammation and blood-retina barrier breakdown in mice and rats","volume":"56","author":"Mysona","year":"2013","journal-title":"Diabetologia"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"15013","DOI":"10.1038\/mtm.2015.13","article-title":"Silencing p75(NTR) prevents proNGF-induced endothelial cell death and development of acellular capillaries in rat retina","volume":"2","author":"Shanab","year":"2015","journal-title":"Mol. Ther Methods Clin. Dev."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Mohamed, R., Shanab, A.Y., and El Remessy, A.B. (2017). Deletion of the Neurotrophin Receptor p75(NTR) Prevents Diabetes-Induced Retinal Acellular Capillaries in Streptozotocin-Induced Mouse Diabetic Model. J. Diabetes Metab. Disord. Control, 4.","DOI":"10.15406\/jdmdc.2017.04.00129"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"12490","DOI":"10.1038\/s41598-018-30029-0","article-title":"Deletion of p75(NTR) prevents vaso-obliteration and retinal neovascularization via activation of Trk- A receptor in ischemic retinopathy model","volume":"8","author":"Elshaer","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"8826","DOI":"10.1523\/JNEUROSCI.4278-15.2016","article-title":"p75NTR and Its Ligand ProNGF Activate Paracrine Mechanisms Etiological to the Vascular, Inflammatory, and Neurodegenerative Pathologies of Diabetic Retinopathy","volume":"36","author":"Barcelona","year":"2016","journal-title":"J. Neurosci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.neulet.2007.10.012","article-title":"Enhanced protein expressions of sortilin and p75NTR in retina of rat following elevated intraocular pressure-induced retinal ischemia","volume":"429","author":"Wei","year":"2007","journal-title":"Neurosci. Lett."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/nrn1726","article-title":"The yin and yang of neurotrophin action","volume":"6","author":"Lu","year":"2005","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1634\/stemcells.2006-0494","article-title":"Identification of human oral keratinocyte stem\/progenitor cells by neurotrophin receptor p75 and the role of neurotrophin\/p75 signaling","volume":"25","author":"Nakamura","year":"2007","journal-title":"Stem Cells"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"7558","DOI":"10.1074\/jbc.275.11.7558","article-title":"Nerve growth factor activation of nuclear factor kappaB through its p75 receptor is an anti-apoptotic signal in RN22 schwannoma cells","volume":"275","author":"Gentry","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0079-6123(03)46003-2","article-title":"The role of neurotransmission and the Chopper domain in p75 neurotrophin receptor death signaling","volume":"146","author":"Coulson","year":"2004","journal-title":"Prog. Brain Res."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Johnston, A.L., Lun, X., Rahn, J.J., Liacini, A., Wang, L., Hamilton, M.G., Parney, I.F., Hempstead, B.L., Robbins, S.M., and Forsyth, P.A. (2007). The p75 neurotrophin receptor is a central regulator of glioma invasion. PLoS Biol., 5.","DOI":"10.1371\/journal.pbio.0050212"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1007\/s00125-019-4885-2","article-title":"Modulation of the p75 neurotrophin receptor using LM11A-31 prevents diabetes-induced retinal vascular permeability in mice via inhibition of inflammation and the RhoA kinase pathway","volume":"62","author":"Elshaer","year":"2019","journal-title":"Diabetologia"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.preteyeres.2011.02.001","article-title":"Vascular stem cells and ischaemic retinopathies","volume":"30","author":"Stitt","year":"2011","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/S0301-472X(02)00812-3","article-title":"Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factor receptor antibodies","volume":"30","author":"Quirici","year":"2002","journal-title":"Exp. Hematol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1007\/s00417-006-0382-7","article-title":"Transplantation of bone marrow-derived mesenchymal stem cells rescue photoreceptor cells in the dystrophic retina of the rhodopsin knockout mouse","volume":"245","author":"Arnhold","year":"2007","journal-title":"Graefes Arch. Clin. Exp. Ophthalmol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1007\/s00417-008-1009-y","article-title":"Effects of bone-marrow mesenchymal stem cells transplanted into vitreous cavity of rat injured by ischemia\/reperfusion","volume":"247","author":"Li","year":"2009","journal-title":"Graefes Arch. Clin. Exp. Ophthalmol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"601176","DOI":"10.3389\/fncel.2020.601176","article-title":"Mesenchymal Stromal Cell-Derived Extracellular Vesicles Reduce Neuroinflammation, Promote Neural Cell Proliferation and Improve Oligodendrocyte Maturation in Neonatal Hypoxic-Ischemic Brain Injury","volume":"14","author":"Kaminski","year":"2020","journal-title":"Front. Cell Neurosci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1002\/cbin.10515","article-title":"How do culture media influence in vitro perivascular cell behavior?","volume":"39","author":"Huber","year":"2015","journal-title":"Cell Biol. Int."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1096\/fj.201600951RRR","article-title":"A systematic review: Differentiation of stem cells into functional pericytes","volume":"31","author":"Xu","year":"2017","journal-title":"FASEB J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1093\/abbs\/gmy072","article-title":"Abeta inhibits mesenchymal stem cell-pericyte transition through MAPK pathway","volume":"50","author":"Xu","year":"2018","journal-title":"Acta Biochim. Biophys. Sin. (Shanghai)"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.yjmcc.2005.07.003","article-title":"Soluble factors released by endothelial progenitor cells promote migration of endothelial cells and cardiac resident progenitor cells","volume":"39","author":"Urbich","year":"2005","journal-title":"J. Mol. Cell Cardiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.ejcts.2009.04.052","article-title":"Mesenchymal stem cells over-expressing SDF-1 promote angiogenesis and improve heart function in experimental myocardial infarction in rats","volume":"36","author":"Tang","year":"2009","journal-title":"Eur. J. Cardiothorac Surg."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1186\/s12931-017-0620-z","article-title":"Stromal derived factor-1 mediates the lung regenerative effects of mesenchymal stem cells in a rodent model of bronchopulmonary dysplasia","volume":"18","author":"Reiter","year":"2017","journal-title":"Respir. Res."},{"key":"ref_42","first-page":"561098","article-title":"Enhancing the migration ability of mesenchymal stromal cells by targeting the SDF-1\/CXCR4 axis","volume":"2013","year":"2013","journal-title":"Biomed. Res. Int."},{"key":"ref_43","first-page":"1250","article-title":"Enhanced recruitment and hematopoietic reconstitution of bone marrow-derived mesenchymal stem cells in bone marrow failure by the SDF-1\/CXCR4","volume":"14","author":"Chen","year":"2020","journal-title":"J. Tissue Eng. Regen Med."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1038\/s41419-020-2512-2","article-title":"Atypical chemokine receptor ACKR3\/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling","volume":"11","author":"Wei","year":"2020","journal-title":"Cell Death Dis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.brainres.2014.05.035","article-title":"CXCR-7 receptor promotes SDF-1alpha-induced migration of bone marrow mesenchymal stem cells in the transient cerebral ischemia\/reperfusion rat hippocampus","volume":"1575","author":"Wang","year":"2014","journal-title":"Brain Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1007\/s12079-016-0352-8","article-title":"VEGF-A\/VEGFR2 signaling network in endothelial cells relevant to angiogenesis","volume":"10","author":"Abhinand","year":"2016","journal-title":"J. Cell Commun. Signal."},{"key":"ref_47","first-page":"1667","article-title":"VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms","volume":"17","author":"Ge","year":"2018","journal-title":"Mol. Med. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1167\/iovs.61.2.6","article-title":"TNFalpha-Mediated Priming of Mesenchymal Stem Cells Enhances Their Neuroprotective Effect on Retinal Ganglion Cells","volume":"61","author":"Mead","year":"2020","journal-title":"Investig. Ophthalmol Vis. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"398","DOI":"10.3389\/fncel.2019.00398","article-title":"Reversal of Cognitive Impairment in gp120 Transgenic Mice by the Removal of the p75 Neurotrophin Receptor","volume":"13","author":"Speidell","year":"2019","journal-title":"Front. Cell Neurosci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1038\/aps.2011.141","article-title":"Nerve growth factor induces cord formation of mesenchymal stem cell by promoting proliferation and activating the PI3K\/Akt signaling pathway","volume":"32","author":"Wang","year":"2011","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2030","DOI":"10.1167\/iovs.11-8430","article-title":"Nerve growth factor promotes endothelial progenitor cell-mediated angiogenic responses","volume":"53","author":"Jadhao","year":"2012","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1159\/000065081","article-title":"Modulation of Bcl-2 family proteins in primary endothelial cells during apoptosis","volume":"32","author":"Molostvov","year":"2002","journal-title":"Pathophysiol. Haemost. Thromb."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1523\/JNEUROSCI.0399-12.2013","article-title":"Oral administration of a small molecule targeted to block proNGF binding to p75 promotes myelin sparing and functional recovery after spinal cord injury","volume":"33","author":"Tep","year":"2013","journal-title":"J. Neurosci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5288","DOI":"10.1523\/JNEUROSCI.3547-05.2006","article-title":"Small, nonpeptide p75NTR ligands induce survival signaling and inhibit proNGF-induced death","volume":"26","author":"Massa","year":"2006","journal-title":"J. Neurosci."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2052","DOI":"10.1016\/j.neurobiolaging.2013.02.015","article-title":"Small molecule p75NTR ligand prevents cognitive deficits and neurite degeneration in an Alzheimer\u2019s mouse model","volume":"34","author":"Knowles","year":"2013","journal-title":"Neurobiol. Aging"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Coucha, M., Shanab, A.Y., Sayed, M., Vazdarjanova, A., and El-Remessy, A.B. (2019). Modulating Expression of Thioredoxin Interacting Protein (TXNIP) Prevents Secondary Damage and Preserves Visual Function in a Mouse Model of Ischemia\/Reperfusion. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20163969"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"3212","DOI":"10.1089\/ten.tea.2013.0663","article-title":"Total body irradiation is permissive for mesenchymal stem cell-mediated new bone formation following local transplantation","volume":"20","author":"Herberg","year":"2014","journal-title":"Tissue Eng. Part A"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/j.bbadis.2017.12.023","article-title":"Inducible overexpression of endothelial proNGF as a mouse model to study microvascular dysfunction","volume":"1864","author":"Mohamed","year":"2018","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"432659","DOI":"10.1155\/2013\/432659","article-title":"Pronerve growth factor induces angiogenesis via activation of TrkA: Possible role in proliferative diabetic retinopathy","volume":"2013","author":"Elshaer","year":"2013","journal-title":"J. Diabetes Res."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Park, H.S., Ashour, D., Elsharoud, A., Chugh, R.M., Ismail, N., El Andaloussi, A., and Al-Hendy, A. (2019). Towards Cell free Therapy of Premature Ovarian Insufficiency: Human Bone Marrow Mesenchymal Stem Cells Secretome Enhances Angiogenesis in Human Ovarian Microvascular Endothelial Cells. HSOA J. Stem Cells Res. Dev. Ther., 5.","DOI":"10.24966\/SRDT-2060\/100019"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"11568","DOI":"10.1038\/s41598-020-67289-8","article-title":"Angiogenesis Analyzer for ImageJ\u2014A comparative morphometric analysis of \u201cEndothelial Tube Formation Assay\u201d and \u201cFibrin Bead Assay\u201d","volume":"10","author":"Carpentier","year":"2020","journal-title":"Sci. Rep."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/2\/829\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:11:39Z","timestamp":1760159499000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/2\/829"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,15]]},"references-count":61,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,1]]}},"alternative-id":["ijms22020829"],"URL":"https:\/\/doi.org\/10.3390\/ijms22020829","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,15]]}}}