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Herein, we report that crude water extract (CWE) of G. uralensis enhanced myoblast proliferation and differentiation. Pretreatment of mice with the CWE of G. uralensis prior to cardiotoxin-induced muscle injury was found to enhance muscle regeneration by inducing myogenic gene expression and downregulating myostatin expression. Furthermore, this extract reduced nitrotyrosine protein levels and atrophy-related gene expression. Of the five different fractions of the CWE of G. uralensis obtained, the ethyl acetate (EtOAc) fraction more significantly enhanced myoblast proliferation and differentiation than the other fractions. Ten bioactive compounds were isolated from the EtOAc fraction and characterized by GC-MS and NMR. Of these compounds (4-hydroxybenzoic acid, liquiritigenin, (R)-(-)-vestitol, isoliquiritigenin, medicarpin, tetrahydroxymethoxychalcone, licochalcone B, liquiritin, liquiritinapioside, and ononin), liquiritigenin, tetrahydroxymethoxychalcone, and licochalcone B were found to enhance myoblast proliferation and differentiation, and myofiber diameters in injured muscles were wider with the liquiritigenin than the non-treated one. Computational analysis showed these compounds are non-toxic and possess good drug-likeness properties. These findings suggest that G. uralensis-extracted components might be useful therapeutic agents for the management of muscle-associated diseases.<\/jats:p>","DOI":"10.3390\/ijms22020876","type":"journal-article","created":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T05:17:34Z","timestamp":1610947054000},"page":"876","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Isolation and Characterization of Compounds from Glycyrrhiza uralensis as Therapeutic Agents for the Muscle Disorders"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2496-0463","authenticated-orcid":false,"given":"Eun Ju","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, Korea"},{"name":"Research Institute of Cell Culture, Yeungnam University, Gyeongsan 38541, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sibhghatulla","family":"Shaikh","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, Korea"},{"name":"Research Institute of Cell Culture, Yeungnam University, Gyeongsan 38541, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1095-8445","authenticated-orcid":false,"given":"Khurshid","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, Korea"},{"name":"Research Institute of Cell Culture, Yeungnam University, Gyeongsan 38541, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed Sayeed","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, Korea"},{"name":"Research Institute of Cell Culture, Yeungnam University, Gyeongsan 38541, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0375-8170","authenticated-orcid":false,"given":"Jeong Ho","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, Korea"},{"name":"Research Institute of Cell Culture, Yeungnam University, Gyeongsan 38541, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soyoung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine, Yeungnam University, Daegu 42415, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Jin","family":"Yang","sequence":"additional","affiliation":[{"name":"Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 701300, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won-Kyung","family":"Cho","sequence":"additional","affiliation":[{"name":"Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 701300, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang-Joon","family":"Park","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Ho","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science, Daegu Catholic University, Gyeongsan 38430, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So-Young","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine, Yeungnam University, Daegu 42415, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Yeul","family":"Ma","sequence":"additional","affiliation":[{"name":"Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu 701300, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inho","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, Korea"},{"name":"Research Institute of Cell Culture, Yeungnam University, Gyeongsan 38541, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/s00223-014-9915-y","article-title":"Skeletal muscle: A brief review of structure and function","volume":"96","author":"Frontera","year":"2015","journal-title":"Calcif. 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