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Due to the global challenge of antimicrobial resistance, alternative treatment strategies are needed. Here, we investigated whether inhibition of quorum sensing (QS) by sodium salicylate in different P. aeruginosa strains (QS-competent, QS-mutant, and chronic wound strains) influences biofilm formation and tolerance to silver. Biofilm formation was evaluated in simulated serum-containing wound fluid in the presence or absence of sodium salicylate (NaSa). Biofilms were established using a 3D collagen-based biofilm model, collagen coated glass, and the Calgary biofilm device. Furthermore, the susceptibility of 48-h-old biofilms formed by laboratory and clinical strains in the presence or absence of NaSa towards silver was evaluated by assessing cell viability. Biofilms formed in the presence of NaSa were more susceptible to silver and contained reduced levels of virulence factors associated with biofilm development than those formed in the absence of NaSa. Biofilm aggregates formed by the wild-type but not the QS mutant strain, were smaller and less heterogenous in size when grown in cultures with NaSa compared to control. These data suggest that NaSa, via a reduction of cell aggregation in biofilms, allows the antiseptic to become more readily available to cells.<\/jats:p>","DOI":"10.3390\/ijms22031060","type":"journal-article","created":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T11:49:40Z","timestamp":1611229780000},"page":"1060","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Sodium Salicylate Influences the Pseudomonas aeruginosa Biofilm Structure and Susceptibility Towards Silver"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5920-1956","authenticated-orcid":false,"given":"Erik","family":"Gerner","sequence":"first","affiliation":[{"name":"Department of Biomaterials, The Sahlgrenska Academy, University of Gothenburg, 405 30 Gothenburg, Sweden"},{"name":"M\u00f6lnlycke Health Care AB, 415 02 Gothenburg, Sweden"},{"name":"Center for Antibiotic Resistance Research (CARe), University of Gothenburg, 405 30 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1259-6860","authenticated-orcid":false,"given":"Sofia","family":"Almqvist","sequence":"additional","affiliation":[{"name":"M\u00f6lnlycke Health Care AB, 415 02 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3910-6665","authenticated-orcid":false,"given":"Peter","family":"Thomsen","sequence":"additional","affiliation":[{"name":"Department of Biomaterials, The Sahlgrenska Academy, University of Gothenburg, 405 30 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Werth\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Biomaterials, The Sahlgrenska Academy, University of Gothenburg, 405 30 Gothenburg, Sweden"},{"name":"Center for Antibiotic Resistance Research (CARe), University of Gothenburg, 405 30 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9253-6771","authenticated-orcid":false,"given":"Margarita","family":"Trobos","sequence":"additional","affiliation":[{"name":"Department of Biomaterials, The Sahlgrenska Academy, University of Gothenburg, 405 30 Gothenburg, Sweden"},{"name":"Center for Antibiotic Resistance Research (CARe), University of Gothenburg, 405 30 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S38","DOI":"10.1016\/S0002-9610(03)00303-9","article-title":"A specialized wound-healing center concept: Importance of a multidisciplinary department structure and surgical treatment facilities in the treatment of chronic wounds","volume":"187","author":"Gottrup","year":"2004","journal-title":"Am. 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