{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:45:23Z","timestamp":1760233523663,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T00:00:00Z","timestamp":1611273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 CA99908","R01 CA184101"],"award-info":[{"award-number":["R01 CA99908","R01 CA184101"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Department of Obstetrics and Gynecology, University of Iowa","award":["Basic research fund"],"award-info":[{"award-number":["Basic research fund"]}]},{"name":"American Association of Obstetricians and Gynecologists Foundation","award":["Bridge Funding Award"],"award-info":[{"award-number":["Bridge Funding Award"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Long non-coding RNA\u2019s (lncRNA) are RNA sequences that do not encode proteins and are greater than 200 nucleotides in length. They regulate complex cellular mechanisms and have been associated with prognosis in various types of cancer. We aimed to identify lncRNA sequences that are associated with high grade serous ovarian cancer (HGSC) and assess their impact on overall survival. RNA was extracted from 112 HGSC patients and 12 normal fallopian tube samples from our Biobank tissue repository. RNA was sequenced and the Ultrafast and Comprehensive lncRNA detection and quantification pipeline (UClncR) was used for the identification of lncRNA sequences. Univariate logistic and multivariate lasso regression analyses identified lncRNA that was associated with HGSC. Univariate and multivariate Cox proportional hazard ratios were used to evaluate independent predictors of survival. 1943 of 16,325 investigated lncRNA\u2019s were differentially expressed in HGSC as compared to controls (p &lt; 0.001). Nine of these demonstrated association with cancer after multivariate lasso regression. Our multivariate analysis of survival identified four lncRNA\u2019s associated with survival in HGSC. Three out of these four were found to be independently significant after accounting for all clinical covariates. Lastly, seven lncRNAs were independently associated with initial response to chemotherapy; four portended a worse response, while three were associated with improved response. More research is needed, but there is potential for these lncRNAs to be used as biomarkers of HGSC or predictors of treatment outcome in the future.<\/jats:p>","DOI":"10.3390\/ijms22031079","type":"journal-article","created":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T11:13:53Z","timestamp":1611314033000},"page":"1079","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Identification of Novel lncRNAs in Ovarian Cancer and Their Impact on Overall Survival"],"prefix":"10.3390","volume":"22","author":[{"given":"Nicholas","family":"Cardillo","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, IA 52245, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, IA 52245, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7591-2483","authenticated-orcid":false,"given":"Andreea","family":"Newtson","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, IA 52245, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry","family":"Reyes","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology, University of Buffalo, Buffalo, NY 14203, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasmin","family":"Lyons","sequence":"additional","affiliation":[{"name":"Department of Gynecologic Oncology, University of Texas\u2014San Antonio, San Antonio, TX 78229, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7131-8604","authenticated-orcid":false,"given":"Eric","family":"Devor","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, IA 52245, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Bender","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, IA 52245, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Goodheart","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, IA 52245, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2079-4528","authenticated-orcid":false,"given":"Jesus","family":"Gonzalez-Bosquet","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, IA 52245, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,22]]},"reference":[{"key":"ref_1","unstructured":"Howlader, N., Noone, A.M., and Krapcho, M. 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