{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:09:42Z","timestamp":1790190582113,"version":"4.1.0"},"reference-count":62,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T00:00:00Z","timestamp":1612396800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["Discovery Grant"],"award-info":[{"award-number":["Discovery Grant"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008921","name":"Ministry of Agriculture - Saskatchewan","doi-asserted-by":"publisher","award":["ADF"],"award-info":[{"award-number":["ADF"]}],"id":[{"id":"10.13039\/100008921","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Saskatchewan Wheat Development Commission","award":["SWDC-ADF grant"],"award-info":[{"award-number":["SWDC-ADF grant"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cuticular waxes are a mixture of hydrophobic very-long-chain fatty acids and their derivatives accumulated in the plant cuticle. Most studies define the role of cuticular wax largely based on reducing nonstomatal water loss. The present study investigated the role of cuticular wax in reducing both low-temperature and dehydration stress in plants using Arabidopsis thaliana mutants and transgenic genotypes altered in the formation of cuticular wax. cer3-6, a known Arabidopsis wax-deficient mutant (with distinct reduction in aldehydes, n-alkanes, secondary n-alcohols, and ketones compared to wild type (WT)), was most sensitive to water loss, while dewax, a known wax overproducer (greater alkanes and ketones compared to WT), was more resistant to dehydration compared to WT. Furthermore, cold-acclimated cer3-6 froze at warmer temperatures, while cold-acclimated dewax displayed freezing exotherms at colder temperatures compared to WT. Gas Chromatography-Mass Spectroscopy (GC-MS) analysis identified a characteristic decrease in the accumulation of certain waxes (e.g., alkanes, alcohols) in Arabidopsis cuticles under cold acclimation, which was additionally reduced in cer3-6. Conversely, the dewax mutant showed a greater ability to accumulate waxes under cold acclimation. Fourier Transform Infrared Spectroscopy (FTIR) also supported observations in cuticular wax deposition under cold acclimation. Our data indicate cuticular alkane waxes along with alcohols and fatty acids can facilitate avoidance of both ice formation and leaf water loss under dehydration stress and are promising genetic targets of interest.<\/jats:p>","DOI":"10.3390\/ijms22041554","type":"journal-article","created":{"date-parts":[[2021,2,4]],"date-time":"2021-02-04T12:02:10Z","timestamp":1612440130000},"page":"1554","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Dissecting the Roles of Cuticular Wax in Plant Resistance to Shoot Dehydration and Low-Temperature Stress in Arabidopsis"],"prefix":"10.3390","volume":"22","author":[{"given":"Tawhidur","family":"Rahman","sequence":"first","affiliation":[{"name":"College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxuan","family":"Shao","sequence":"additional","affiliation":[{"name":"College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shankar","family":"Pahari","sequence":"additional","affiliation":[{"name":"Saskatoon Research and Development Centre, Agriculture and Agri-Food Canada, Saskatoon, SK S7N 0X2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prakash","family":"Venglat","sequence":"additional","affiliation":[{"name":"College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9345-9640","authenticated-orcid":false,"given":"Raju","family":"Soolanayakanahally","sequence":"additional","affiliation":[{"name":"Saskatoon Research and Development Centre, Agriculture and Agri-Food Canada, Saskatoon, SK S7N 0X2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3006-899X","authenticated-orcid":false,"given":"Abidur","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Plant BioSciences, Faculty of Agriculture, Iwate University, Morioka, Iwate 0208550, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Tanino","sequence":"additional","affiliation":[{"name":"College of Agriculture and Bioresources, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1007\/s00299-015-1772-2","article-title":"Advances in the understanding of cuticular waxes in Arabidopsis thaliana and crop species","volume":"34","author":"Lee","year":"2015","journal-title":"Plant Cell Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"621","DOI":"10.3389\/fpls.2017.00621","article-title":"Molecular and evolutionary mechanisms of cuticular wax for plant drought tolerance","volume":"8","author":"Xue","year":"2017","journal-title":"Front. 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