{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T09:51:36Z","timestamp":1790934696057,"version":"4.1.0"},"reference-count":64,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,2,15]],"date-time":"2021-02-15T00:00:00Z","timestamp":1613347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI14\/01956"],"award-info":[{"award-number":["PI14\/01956"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002924","name":"Federaci\u00f3n Espa\u00f1ola de Enfermedades Raras","doi-asserted-by":"publisher","award":["PI18\/00316"],"award-info":[{"award-number":["PI18\/00316"]}],"id":[{"id":"10.13039\/501100002924","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002924","name":"Federaci\u00f3n Espa\u00f1ola de Enfermedades Raras","doi-asserted-by":"publisher","award":["PI17\/00050"],"award-info":[{"award-number":["PI17\/00050"]}],"id":[{"id":"10.13039\/501100002924","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007801","name":"Fundaci\u00f3n S\u00e9neca","doi-asserted-by":"publisher","award":["19873\/GERM\/15"],"award-info":[{"award-number":["19873\/GERM\/15"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Exosomes are extracellular vesicles that contain nucleic acids, lipids and metabolites, and play a critical role in health and disease as mediators of intercellular communication. The majority of extracellular vesicles in the blood are platelet-derived. Compared to adults, neonatal platelets are hyporeactive and show impaired granule release, associated with defects in Soluble N-ethylmaleimide-sensitive fusion Attachment protein REceptor (SNARE) proteins. Since these proteins participate in biogenesis of exosomes, we investigated the potential differences between newborn and adult plasma-derived exosomes. Plasma-derived exosomes were isolated by ultracentrifugation of umbilical cord blood from full-term neonates or peripheral blood from adults. Exosome characterization included size determination by transmission electron microscopy and quantitative proteomic analysis. Plasma-derived exosomes from neonates were significantly smaller and contained 65% less protein than those from adults. Remarkably, 131 proteins were found to be differentially expressed, 83 overexpressed and 48 underexpressed in neonatal (vs. adult) exosomes. Whereas the upregulated proteins in plasma exosomes from neonates are associated with platelet activation, coagulation and granule secretion, most of the underexpressed proteins are immunoglobulins. This is the first study showing that exosome size and content change with age. Our findings may contribute to elucidating the potential \u201cdevelopmental hemostatic mismatch risk\u201d associated with transfusions containing plasma exosomes from adults.<\/jats:p>","DOI":"10.3390\/ijms22041926","type":"journal-article","created":{"date-parts":[[2021,2,15]],"date-time":"2021-02-15T22:58:01Z","timestamp":1613429881000},"page":"1926","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Qualitative and Quantitative Comparison of Plasma Exosomes from Neonates and Adults"],"prefix":"10.3390","volume":"22","author":[{"given":"Julia","family":"Pe\u00f1as-Mart\u00ednez","sequence":"first","affiliation":[{"name":"Servicio de Hematolog\u00eda y Oncolog\u00eda M\u00e9dica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonaci\u00f3n, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda N.","family":"Barrachina","sequence":"additional","affiliation":[{"name":"Platelet Proteomics Group, Centro Singular de Investigaci\u00f3n en Medicina Molecular y Enfermedades Cr\u00f3nicas (CIMUS), Universidad de Santiago de Compostela e Instituto de Investigaci\u00f3n Sanitaria de Santiago (IDIS), 15706 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3601-4549","authenticated-orcid":false,"given":"Ernesto Jos\u00e9","family":"Cuenca-Zamora","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda y Oncolog\u00eda M\u00e9dica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonaci\u00f3n, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9940-0415","authenticated-orcid":false,"given":"Gin\u00e9s","family":"Luengo-Gil","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda y Oncolog\u00eda M\u00e9dica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonaci\u00f3n, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, Spain"},{"name":"Grupo de Investigaci\u00f3n en Patolog\u00eda Molecular y Farmacogen\u00e9tica, Departamento de Dermatolog\u00eda, Estomatolog\u00eda, Radiolog\u00eda y Medicina F\u00edsica, Hospital General Universitario Santa Luc\u00eda, Universidad de Murcia, IMIB-Arrixaca, 30202 Cartagena, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7615-3079","authenticated-orcid":false,"given":"Susana Bel\u00e9n","family":"Bravo","sequence":"additional","affiliation":[{"name":"Servicio de Proteomica, e Instituto de Investigaci\u00f3n Sanitaria de Santiago de Compostela (IDIS), Hospital Cl\u00ednicoUniversitario de Santiago de Compostela, 15706 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Caparr\u00f3s-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda y Oncolog\u00eda M\u00e9dica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonaci\u00f3n, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6741-9725","authenticated-orcid":false,"given":"Ra\u00fal","family":"Teruel-Montoya","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda y Oncolog\u00eda M\u00e9dica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonaci\u00f3n, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, Spain"},{"name":"U-765-CIBERER, Instituto de Salud Carlos III (ISCIII), 28220 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0511-3533","authenticated-orcid":false,"given":"Jos\u00e9","family":"Eliseo-Blanco","sequence":"additional","affiliation":[{"name":"Servicio de Obstetricia y Ginecolog\u00eda, Hospital Cl\u00ednico Virgen de la Arrixaca, 30120 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4278-3264","authenticated-orcid":false,"given":"Vicente","family":"Vicente","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda y Oncolog\u00eda M\u00e9dica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonaci\u00f3n, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, Spain"},{"name":"U-765-CIBERER, Instituto de Salud Carlos III (ISCIII), 28220 Madrid, 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Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9520-3243","authenticated-orcid":false,"given":"Francisca","family":"Ferrer-Mar\u00edn","sequence":"additional","affiliation":[{"name":"Servicio de Hematolog\u00eda y Oncolog\u00eda M\u00e9dica, Hospital Universitario Morales Meseguer, Centro Regional de Hemodonaci\u00f3n, Universidad de Murcia, IMIB-Arrixaca, 30003 Murcia, Spain"},{"name":"U-765-CIBERER, Instituto de Salud Carlos III (ISCIII), 28220 Madrid, Spain"},{"name":"Grado de Medicina, Universidad Cat\u00f3lica San Antonio de Murcia, 30107 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1182\/asheducation.V2012.1.506.3798532","article-title":"Platelets in the neonatal period: Developmental differences in platelet production, function, and hemostasis and the potential impact of therapies","volume":"2012","year":"2012","journal-title":"Hematol. Am. Soc. Hematol. Educ. Program"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1159\/000481016","article-title":"Decreased alpha-adrenergic receptors in newborn platelets: Cause of abnormal response to epinephrine","volume":"2","author":"Corby","year":"1981","journal-title":"Dev. Pharm."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1203\/00006450-199604000-00022","article-title":"Impaired mobilization of intracellular calcium in neonatal platelets","volume":"39","author":"Gelman","year":"1996","journal-title":"Pediatr. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1203\/00006450-199905010-00014","article-title":"Impaired signal transduction in neonatal platelets","volume":"45","author":"Israels","year":"1999","journal-title":"Pediatr. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"641","DOI":"10.3109\/09537104.2010.504869","article-title":"Newborn platelets: Lower levels of protease-activated receptors cause hypoaggregability to thrombin","volume":"21","author":"Schlagenhauf","year":"2010","journal-title":"Platelets"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1159\/000253755","article-title":"Closure times measured by the platelet function analyzer PFA-100\u00ae Are longer in neonatal blood compared to cord blood samples","volume":"97","author":"Saxonhouse","year":"2010","journal-title":"Neonatology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1080\/09537100400016862","article-title":"Neonatal platelets from cord blood and peripheral blood","volume":"16","author":"Sitaru","year":"2005","journal-title":"Platelets"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1182\/blood-2014-08-531582","article-title":"Platelets at the interface of thrombosis, inflammation, and cancer","volume":"126","author":"Franco","year":"2015","journal-title":"Blood"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2814","DOI":"10.1111\/trf.12343","article-title":"Distinct differences in platelet production and function between neonates and adults: Implications for platelet transfusion practice","volume":"53","author":"Stanworth","year":"2013","journal-title":"Transfusion"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3381","DOI":"10.1182\/blood-2013-06-508200","article-title":"Expansion of the neonatal platelet mass is achieved via an extension of platelet lifespan","volume":"123","author":"Liu","year":"2014","journal-title":"Blood"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1111\/jth.13270","article-title":"Assessment of neonatal platelet adhesion, activation, and aggregation","volume":"14","author":"Lattimore","year":"2016","journal-title":"J. Thromb. Haemost."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1182\/bloodadvances.2018024372","article-title":"Ontogeny of platelet function","volume":"3","author":"Margraf","year":"2019","journal-title":"Blood Adv."},{"key":"ref_13","first-page":"15","article-title":"Developmental differences in platelet inhibition response to prostaglandin E1","volume":"117","author":"Torregrosa","year":"2019","journal-title":"Neonatology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e36","DOI":"10.3324\/haematol.2016.152421","article-title":"Decreased numbers of dense granules in fetal and neonatal platelets","volume":"102","author":"Urban","year":"2017","journal-title":"Haematologica"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2079","DOI":"10.1160\/TH17-04-0241","article-title":"Down regulation of the Munc18b-syntaxin-11 complex and \u03921-Tubulin impairs secretion and spreading in neonatal platelets","volume":"117","author":"Torregrosa","year":"2017","journal-title":"Thromb. Haemost."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"120","DOI":"10.3109\/09687688.2015.1079934","article-title":"A Unique SNARE Machinery for Exocytosis of cytotoxic granules and platelets granules","volume":"32","author":"Tang","year":"2015","journal-title":"Mol. Membr. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1097\/MOH.0b013e328309ec74","article-title":"The platelet release reaction: Just when you thought platelet secretion was simple","volume":"15","author":"Ren","year":"2008","journal-title":"Curr. Opin. Hematol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1016\/j.bbamcr.2009.09.011","article-title":"TI-VAMP\/VAMP7 and VAMP3\/Cellubrevin: Two v-SNARE proteins involved in specific steps of the autophagy\/multivesicular body pathways","volume":"1793","author":"Fader","year":"2009","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/nrd3978","article-title":"Extracellular vesicles: Biology and emerging therapeutic opportunities","volume":"12","author":"Breakefield","year":"2013","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1038\/nri3622","article-title":"Regulation of immune responses by extracellular vesicles","volume":"14","author":"Robbins","year":"2014","journal-title":"Nat. Rev. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1182\/blood-2012-07-442756","article-title":"SNARing platelet granule secretion","volume":"120","author":"Marks","year":"2012","journal-title":"Blood"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.1111\/tra.12337","article-title":"Munc18-2 is required for syntaxin 11 localization on the plasma membrane in cytotoxic T-lymphocytes","volume":"16","author":"Dieckmann","year":"2015","journal-title":"Traffic"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"E2176","DOI":"10.1073\/pnas.1617981114","article-title":"SM protein munc18-2 facilitates transition of syntaxin 11-mediated lipid mixing to complete fusion for t-lymphocyte cytotoxicity","volume":"114","author":"Spessott","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1146\/annurev-cellbio-101512-122326","article-title":"Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles","volume":"30","author":"Colombo","year":"2014","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1080\/09537104.2016.1268255","article-title":"Extracellular vesicles from activated platelets: A semiquantitative cryo-electron microscopy and immuno-gold labeling study","volume":"28","author":"Brisson","year":"2017","journal-title":"Platelets"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1373\/clinchem.2007.097972","article-title":"Detection and characterization of placental microRNAs in maternal plasma","volume":"54","author":"Chim","year":"2008","journal-title":"Clin. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1055\/s-0037-1604092","article-title":"Vitamin K-dependent protein S: Beyond the protein C pathway","volume":"44","year":"2018","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3402\/jev.v2i0.20677","article-title":"Distinct RNA profiles in subpopulations of extracellular vesicles: Apoptotic bodies, microvesicles and exosomes","volume":"2","author":"Crescitelli","year":"2013","journal-title":"J. Extracell. Vesicles"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13578-019-0282-2","article-title":"Exosomes: Biogenesis, biologic function and clinical potential","volume":"9","author":"Zhang","year":"2019","journal-title":"Cell Biosci."},{"key":"ref_30","first-page":"1","article-title":"Differential MiRNA expression profile and proteome in plasma exosomes from patients with paroxysmal nocturnal hemoglobinuria","volume":"9","author":"Vallejo","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.thromres.2019.10.001","article-title":"First exploratory study on the metabolome from plasma exosomes in patients with paroxysmal nocturnal hemoglobinuria","volume":"183","author":"Vallejo","year":"2019","journal-title":"Thromb. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3791","DOI":"10.1182\/blood.V94.11.3791","article-title":"Activated platelets release two types of membrane vesicles: Microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and a-granules","volume":"94","author":"Heijnen","year":"1999","journal-title":"Blood J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2359","DOI":"10.1056\/NEJMcibr1503100","article-title":"Expanding on exosomes and ectosomes in cancer","volume":"372","year":"2015","journal-title":"N. Engl. J. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1038\/35057024","article-title":"A genomic perspective on membrane compartment organization","volume":"409","author":"Bock","year":"2001","journal-title":"Nature"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"7","DOI":"10.5772\/61186","article-title":"Exosomes: Mechanisms of uptake","volume":"4","author":"McKelvey","year":"2015","journal-title":"J. Circ. Biomark."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Caparr\u00f3s-P\u00e9rez, E., Teruel-Montoya, R., L\u00f3pez-Andreo, M.J., Llanos, M.C., Rivera, J., Palma-Barqueros, V., Blanco, J.E., Vicente, V., Mart\u00ednez, C., and Ferrer-Mar\u00edn, F. (2017). Comprehensive comparison of neonate and adult human platelet transcriptomes. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0183042"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1055\/s-0038-1646924","article-title":"Significant hypo-responsiveness to GPVI and CLEC-2 agonists in pre-term and full-term neonatal platelets and following immune thrombocytopenia","volume":"118","author":"Hardy","year":"2018","journal-title":"Thromb. Haemost."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1126\/science.aau6977","article-title":"The Biology, function, and biomedical applications of exosomes","volume":"367","author":"Kalluri","year":"2020","journal-title":"Science"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1586\/1744666X.2014.942288","article-title":"Immune responses in neonates","volume":"10","author":"Basha","year":"2014","journal-title":"Expert Rev. Clin. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.thromres.2009.11.023","article-title":"Role of the platelet chemokine platelet factor 4 (PF4) in hemostasis and thrombosis","volume":"125","author":"Kowalska","year":"2010","journal-title":"Thromb. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1161\/01.RES.0000251742.71301.16","article-title":"Activation of platelet function through G protein-coupled receptors","volume":"99","author":"Offermanns","year":"2006","journal-title":"Circ. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1055\/s-2003-42584","article-title":"Developmental hemostasis: Pro- and anticoagulant systems during childhood","volume":"29","author":"Kuhle","year":"2003","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1159\/000242646","article-title":"Elevated levels of A2-Macroglobulin-protease complexes in infants","volume":"51","author":"Levine","year":"1987","journal-title":"Biol. Neonate"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.ejogrb.2011.04.026","article-title":"Microparticles (MPs), Tissue Factor (TF) and Tissue Factor Inhibitor (TFPI) in cord blood plasma. A preliminary study and literature survey of procoagulant properties of MPs","volume":"158","author":"Zekanowska","year":"2011","journal-title":"Eur. J. Obs. Gynecol. Reprod. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.thromres.2011.01.013","article-title":"Microparticles in newborn cord blood: Slight elevation after normal delivery","volume":"128","author":"Schweintzger","year":"2011","journal-title":"Thromb. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1056\/NEJMoa1807320","article-title":"Randomized trial of platelet-transfusion thresholds in neonates","volume":"380","author":"Curley","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1111\/j.1538-7836.2011.04233.x","article-title":"Effects of in vitro adult platelet transfusions on neonatal hemostasis","volume":"9","author":"Chavda","year":"2011","journal-title":"J. Thromb. Haemost."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1647027","DOI":"10.1080\/20013078.2019.1647027","article-title":"Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles","volume":"8","author":"Clayton","year":"2019","journal-title":"J. Extracell. Vesicles"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Maisano, D., Mimmi, S., Russo, R., Fioravanti, A., Fiume, G., Vecchio, E., Nistic\u00f2, N., Quinto, I., and Iaccino, E. (2020). Uncovering the exosomes diversity: A window of opportunity for tumor progression monitoring. Pharmaceuticals, 13.","DOI":"10.3390\/ph13080180"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1042\/bst0240893","article-title":"A strategy for identifying gel-separated proteins in sequence databases by MS alone","volume":"24","author":"Shevchenko","year":"1996","journal-title":"Biochem. Soc. Trans."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"10512","DOI":"10.1002\/jcp.27723","article-title":"Cholinergic activity regulates the secretome of epicardial adipose tissue: Association with atrial fibrillation","volume":"234","author":"Fernandez","year":"2019","journal-title":"J. Cell. Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.cca.2019.10.027","article-title":"Potential clinical applications of the personalized, disease-specific protein corona on nanoparticles","volume":"501","author":"Bravo","year":"2020","journal-title":"Clin. Chim. Acta"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Tamara, C., Nerea, L.B., Bel\u00e9n, B.S., Aurelio, S., Iv\u00e1n, C., Fernando, S., Javier, B., Felipe, C.F., and Mar\u00eda, P. (2020). Vesicles Shed by pathological murine adipocytes spread pathology: Characterization and functional role of insulin resistant\/hypertrophied adiposomes. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21062252"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jprot.2016.07.020","article-title":"Proteomic identification of putative biomarkers for early detection of sudden cardiac death in a family with a LMNA gene mutation causing dilated cardiomyopathy","volume":"148","author":"Izquierdo","year":"2016","journal-title":"J. Proteom."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Del Pilar Chantada-V\u00e1zquez, M., L\u00f3pez, A.C., Vence, M.G., V\u00e1zquez-Est\u00e9vez, S., Acea-Nebril, B., Calatayud, D.G., Jardiel, T., Bravo, S.B., and N\u00fa\u00f1ez, C. (2020). Proteomic investigation on bio-corona of Au, Ag and Fe nanoparticles for the discovery of triple negative breast cancer serum protein biomarkers. J. Proteom., 212.","DOI":"10.1016\/j.jprot.2019.103581"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Chantada-V\u00e1zquez, M.D.P., L\u00f3pez, A.C., Garc\u00eda-Vence, M., Acea-Nebril, B., Bravo, S.B., and N\u00fa\u00f1ez, C. (2020). Protein corona gold nanoparticles fingerprinting reveals a profile of blood coagulation proteins in the serum of Her2-overexpressing breast cancer patients. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21228449"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Chantada-V\u00e1zquez, M.D.P., Garc\u00eda-Vence, M., V\u00e1zquez-Est\u00e9vez, S., Bravo, S.B., and N\u00fa\u00f1ez, C. (2020). Identification of a profile of neutrophil-derived granule proteins in the surface of gold nanoparticles after their interaction with human breast cancer sera. Nanomaterials, 10.","DOI":"10.3390\/nano10061223"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Hermida-Nogueira, L., Barrachina, M.N., Izquierdo, I., Garc\u00eda-Vence, M., Lacerenza, S., Bravo, S., Castrillo, A., and Garc\u00eda, \u00c1. (2020). Proteomic Analysis of extracellular vesicles derived from platelet concentrates treated with mirasol\u00ae identifies biomarkers of platelet storage lesion. J. Proteom., 210.","DOI":"10.1016\/j.jprot.2019.103529"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1038\/nmeth.2702","article-title":"Mapping differential interactomes by affinity purification coupled with data independent mass spectrometry acquisition","volume":"10","author":"Lambert","year":"2014","journal-title":"Nat. Methods"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Vence, M., Chantada-V\u00e1zquez, M.D.P., Cameselle-Teijeiro, J.M., Bravo, S.B., and N\u00fa\u00f1ez, C. (2020). A Novel nanoproteomic approach for the identification of molecular targets associated with thyroid tumors. Nanomaterials, 10.","DOI":"10.3390\/nano10122370"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Varela-Rodr\u00edguez, B.M., Juiz-Vali\u00f1a, P., Varela, L., Outeiri\u00f1o-Blanco, E., Bravo, S.B., Garc\u00eda-Brao, M.J., Mena, E., Noguera, J.F., Valero-Gasalla, J., and Cordido, F. (2020). Beneficial effects of bariatric surgery-induced by weight loss on the proteome of abdominal subcutaneous adipose tissue. J. Clin. Med., 9.","DOI":"10.3390\/jcm9010213"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"7974","DOI":"10.1021\/ac901143w","article-title":"Compensation for systematic cross-contribution improves normalization of mass spectrometry based metabolomics Data","volume":"81","author":"Redestig","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2597","DOI":"10.1002\/pmic.201400515","article-title":"FunRich: An open access standalone functional enrichment and interaction network analysis tool","volume":"15","author":"Pathan","year":"2015","journal-title":"Proteomics"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1321455","DOI":"10.1080\/20013078.2017.1321455","article-title":"A Novel community driven software for functional enrichment analysis of extracellular vesicles data","volume":"6","author":"Pathan","year":"2017","journal-title":"J. Extracell. 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