{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T10:35:50Z","timestamp":1792060550512,"version":"4.4.0"},"reference-count":48,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,2,16]],"date-time":"2021-02-16T00:00:00Z","timestamp":1613433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"POE FEDER 2014-20 of the Conseil G\u00e9n\u00e9ral de La R\u00e9union","award":["ZIKAlert program, N\u00b0 SYNERGIE: RE00001902"],"award-info":[{"award-number":["ZIKAlert program, N\u00b0 SYNERGIE: RE00001902"]}]},{"name":"POE FEDER 2014-20 of the Conseil G\u00e9n\u00e9ral de La R\u00e9union","award":["RUNDENG program, N\u00b0 SYNERGIE: RE0000xxxx"],"award-info":[{"award-number":["RUNDENG program, N\u00b0 SYNERGIE: RE0000xxxx"]}]},{"name":"POE FEDER 2014-20 of the Conseil G\u00e9n\u00e9ral de La R\u00e9union","award":["DIRED\/20181189"],"award-info":[{"award-number":["DIRED\/20181189"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>La Reunion island in the South West Indian Ocean is now endemic for dengue following the introduction of dengue virus serotype 2 (DENV-2) cosmopolitan-I genotype in 2017. DENV-2 infection causes a wide spectrum of clinical manifestations ranging from flu-like disease to severe dengue. The nonstructural glycoprotein 1 (NS1) has been identified as playing a key role in dengue disease severity. The intracellular NS1 exists as a homodimer, whereas a fraction is driven towards the plasma membrane or released as a soluble hexameric protein. Here, we characterized the NS1 glycoproteins from clinical isolates DES-14 and RUN-18 that were collected during the DENV-2 epidemics in Tanzania in 2014 and La Reunion island in 2018, respectively. In relation to hepatotropism of the DENV, expression of recombinant DES-14 NS1 and RUN-18 NS1 glycoproteins was compared in human hepatoma Huh7 cells. We observed that RUN-18 NS1 was poorly stable in Huh7 cells compared to DES-14 NS1. The instability of RUN-18 NS1 leading to a low level of NS1 secretion mostly relates to lysine residues on positions 272 and 324. Our data raise the issue of the consequences of a defect in NS1 stability in human hepatocytes in relation to the major role of NS1 in the pathogenesis of the DENV-2 infection.<\/jats:p>","DOI":"10.3390\/ijms22041951","type":"journal-article","created":{"date-parts":[[2021,2,16]],"date-time":"2021-02-16T08:09:09Z","timestamp":1613462949000},"page":"1951","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Instability of the NS1 Glycoprotein from La Reunion 2018 Dengue 2 Virus (Cosmopolitan-1 Genotype) in Huh7 Cells Is Due to Lysine Residues on Positions 272 and 324"],"prefix":"10.3390","volume":"22","author":[{"given":"Eva","family":"Ogire","sequence":"first","affiliation":[{"name":"Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Universit\u00e9 de La R\u00e9union, Inserm UMR 1187, CNRS 9192, IRD 249, Plateforme CYROI, 97490 Sainte-Clotilde, Ile de La R\u00e9union, France"},{"name":"Centre International de Recherche en Infectiologie (CIRI), Universit\u00e9 de Lyon, Ecole Normale Sup\u00e9rieure de Lyon, Inserm, U1111, CNRS, UMR5308, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3451-9914","authenticated-orcid":false,"given":"Olivier","family":"Diaz","sequence":"additional","affiliation":[{"name":"Centre International de Recherche en Infectiologie (CIRI), Universit\u00e9 de Lyon, Ecole Normale Sup\u00e9rieure de Lyon, Inserm, U1111, CNRS, UMR5308, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3082-7407","authenticated-orcid":false,"given":"Pierre-Olivier","family":"Vidalain","sequence":"additional","affiliation":[{"name":"Centre International de Recherche en Infectiologie (CIRI), Universit\u00e9 de Lyon, Ecole Normale Sup\u00e9rieure de Lyon, Inserm, U1111, CNRS, UMR5308, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0997-3282","authenticated-orcid":false,"given":"Vincent","family":"Lotteau","sequence":"additional","affiliation":[{"name":"Centre International de Recherche en Infectiologie (CIRI), Universit\u00e9 de Lyon, Ecole Normale Sup\u00e9rieure de Lyon, Inserm, U1111, CNRS, UMR5308, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8926-4050","authenticated-orcid":false,"given":"Philippe","family":"Despr\u00e8s","sequence":"additional","affiliation":[{"name":"Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Universit\u00e9 de La R\u00e9union, Inserm UMR 1187, CNRS 9192, IRD 249, Plateforme CYROI, 97490 Sainte-Clotilde, Ile de La R\u00e9union, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjolaine","family":"Roche","sequence":"additional","affiliation":[{"name":"Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Universit\u00e9 de La R\u00e9union, Inserm UMR 1187, CNRS 9192, IRD 249, Plateforme CYROI, 97490 Sainte-Clotilde, Ile de La R\u00e9union, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e01455","DOI":"10.1016\/j.heliyon.2019.e01455","article-title":"The epidemic of Dengue virus type-2 Cosmopolitan genotype on Reunion Island relates to its active circulation in the Southwestern Indian Ocean neighboring islands","volume":"5","author":"Pascalis","year":"2019","journal-title":"Heliyon"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"ref_3","unstructured":"World Health Organization (WHO) (2009). 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