{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T07:24:42Z","timestamp":1789197882769,"version":"build-2803163510"},"reference-count":40,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,2,17]],"date-time":"2021-02-17T00:00:00Z","timestamp":1613520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2016K1A1A2912755"],"award-info":[{"award-number":["2016K1A1A2912755"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2017R1A2B2002537"],"award-info":[{"award-number":["2017R1A2B2002537"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["018R1A1A1A05018328"],"award-info":[{"award-number":["018R1A1A1A05018328"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2018R1D1A1B07041657"],"award-info":[{"award-number":["2018R1D1A1B07041657"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009624","name":"Yonsei University College of Dentistry","doi-asserted-by":"publisher","award":["6-2019-0006"],"award-info":[{"award-number":["6-2019-0006"]}],"id":[{"id":"10.13039\/501100009624","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>After avulsion and replantation, teeth are at risk of bone and root resorption. The present study aimed to demonstrate that the intra-nuclear transducible form of transcription modulation domain of p65 (nt-p65-TMD) can suppress osteoclast differentiation in vitro, and reduce bone resorption in a rat model of tooth replantation. Cell viability and nitric oxide release were evaluated in RAW264.7 cells using CCK-8 assay and Griess reaction kit. Osteoclast differentiation was evaluated using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and tartrate-resistant acid phosphatase (TRAP) staining. Thirty-two maxillary rat molars were extracted and stored in saline (n = 10) or 10 \u00b5M nt-p65-TMD solution (n = 22) before replantation. After 4 weeks, specimens were scored according to the inflammatory pattern using micro-computed tomography (CT) imaging and histological analyses. nt-p65-TMD treatment resulted in significant reduction of nitric oxide release and osteoclast differentiation as studied using PCR and TRAP staining. Further, micro-CT analysis revealed a significant decrease in bone resorption in the nt-p65-TMD treatment group (p &lt; 0.05). Histological analysis of nt-p65-TMD treatment group showed that not only bone and root resorption, but also inflammation of the periodontal ligament and epithelial insertion was significantly reduced. These findings suggest that nt-p65-TMD has the unique capabilities of regulating bone remodeling after tooth replantation.<\/jats:p>","DOI":"10.3390\/ijms22041987","type":"journal-article","created":{"date-parts":[[2021,2,17]],"date-time":"2021-02-17T21:35:42Z","timestamp":1613597742000},"page":"1987","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Intranuclear Delivery of Nuclear Factor-Kappa B p65 in a Rat Model of Tooth Replantation"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7813-3741","authenticated-orcid":false,"given":"Chung-Min","family":"Kang","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul 03722, Korea"},{"name":"Oral Science Research Center, College of Dentistry, Yonsei University, Seoul 03722, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seunghan","family":"Mo","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul 03722, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mijeong","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul 03722, Korea"},{"name":"Oral Science Research Center, College of Dentistry, Yonsei University, Seoul 03722, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ui-Won","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Periodontology, College of Dentistry, Yonsei University, Seoul 03722, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1121-2570","authenticated-orcid":false,"given":"Yooseok","family":"Shin","sequence":"additional","affiliation":[{"name":"Oral Science Research Center, College of Dentistry, Yonsei University, Seoul 03722, Korea"},{"name":"Department of Conservative Dentistry, College of Dentistry, Yonsei University, Seoul 03722, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Su","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo-Young","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang-Kyou","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung-Jun","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul 03722, Korea"},{"name":"Oral Science Research Center, College of Dentistry, Yonsei University, Seoul 03722, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8620-5629","authenticated-orcid":false,"given":"Je Seon","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul 03722, Korea"},{"name":"Oral Science Research Center, College of Dentistry, Yonsei University, Seoul 03722, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1111\/j.1600-9657.2008.00555.x","article-title":"Treatment of root surface in delayed tooth replantation: A review of literature","volume":"24","author":"Panzarini","year":"2008","journal-title":"Dent. 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