{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T22:57:16Z","timestamp":1785884236779,"version":"3.56.0"},"reference-count":35,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,2,20]],"date-time":"2021-02-20T00:00:00Z","timestamp":1613779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Health, Czech Republic (Conceptual development of research organization, the General University Hospital, Prague)","award":["VFN64165"],"award-info":[{"award-number":["VFN64165"]}]},{"name":"Ministry of Health, Czech Republic (Conceptual development of research organization, the Thomayer Hospital, Prague)","award":["TN64190"],"award-info":[{"award-number":["TN64190"]}]},{"name":"the Grants Agency of the Ministry of Health","award":["NV19-04-00090 and NV18-04-00179"],"award-info":[{"award-number":["NV19-04-00090 and NV18-04-00179"]}]},{"name":"Charles University (Project Progress)","award":["Q27\/LF1"],"award-info":[{"award-number":["Q27\/LF1"]}]},{"name":"Charles University (GAUK)","award":["142120"],"award-info":[{"award-number":["142120"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Alzheimer\u2019s disease (AD) and sporadic Creutzfeldt\u2013Jakob disease (sCJD) are both characterized by extracellular pathologically conformed aggregates of amyloid proteins\u2014amyloid \u03b2-protein (A\u03b2) and prion protein (PrPSc), respectively. To investigate the potential morphological colocalization of A\u03b2 and PrPSc aggregates, we examined the hippocampal regions (archicortex and neocortex) of 20 subjects with confirmed comorbid AD and sCJD using neurohistopathological analyses, immunohistochemical methods, and confocal fluorescent microscopy. Our data showed that extracellular A\u03b2 and PrPSc aggregates tended to be, in most cases, located separately, and \u201ccompound\u201d plaques were relatively rare. We observed PrPSc plaque-like structures in the periphery of the non-compact parts of A\u03b2 plaques, as well as in tau protein-positive dystrophic structures. The AD ABC score according to the NIA-Alzheimer\u2019s association guidelines, and prion protein subtype with codon 129 methionine\u2013valine (M\/V) polymorphisms in sCJD, while representing key characteristics of these diseases, did not correlate with the morphology of the A\u03b2\/PrPSc co-aggregates. However, our data showed that PrPSc aggregation could dominate during co-aggregation with non-compact A\u03b2 in the periphery of A\u03b2 plaques.<\/jats:p>","DOI":"10.3390\/ijms22042099","type":"journal-article","created":{"date-parts":[[2021,2,21]],"date-time":"2021-02-21T21:15:01Z","timestamp":1613942101000},"page":"2099","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Extracellular Protein Aggregates Colocalization and Neuronal Dystrophy in Comorbid Alzheimer\u2019s and Creutzfeldt\u2013Jakob Disease: A Micromorphological Pilot Study on 20 Brains"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8422-2628","authenticated-orcid":false,"given":"Nikol","family":"Jankovska","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Medicine, Third Faculty of Medicine, Charles University and Thomayer University Hospital, Videnska 800, 4-Krc, 14059 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0786-5029","authenticated-orcid":false,"given":"Tomas","family":"Olejar","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, Third Faculty of Medicine, Charles University and Thomayer University Hospital, Videnska 800, 4-Krc, 14059 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6152-6343","authenticated-orcid":false,"given":"Radoslav","family":"Matej","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Medicine, Third Faculty of Medicine, Charles University and Thomayer University Hospital, Videnska 800, 4-Krc, 14059 Prague, Czech Republic"},{"name":"Department of Pathology, First Faculty of Medicine, Charles University, and General University Hospital, 4-Krc, 14059 Prague, Czech Republic"},{"name":"Department of Pathology, Third Faculty of Medicine, Charles University, and University Hospital Kralovske Vinohrady, 4-Krc, 14059 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,20]]},"reference":[{"key":"ref_1","first-page":"4028","article-title":"Prions mediated neurodegenerative disorders","volume":"19","author":"Huang","year":"2015","journal-title":"Eur. 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