{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T02:35:07Z","timestamp":1790044507005,"version":"4.0.1"},"reference-count":197,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T00:00:00Z","timestamp":1614297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI16-00784; PI20-00716"],"award-info":[{"award-number":["PI16-00784; PI20-00716"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Consejeria de Salud, Junta de Andalucia","award":["PI0026-2017"],"award-info":[{"award-number":["PI0026-2017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Critical limb ischemia (CLI) constitutes the most severe form of peripheral arterial disease (PAD), it is characterized by progressive blockade of arterial vessels, commonly correlated to atherosclerosis. Currently, revascularization strategies (bypass grafting, angioplasty) remain the first option for CLI patients, although less than 45% of them are eligible for surgical intervention mainly due to associated comorbidities. Moreover, patients usually require amputation in the short-term. Angiogenic cell therapy has arisen as a promising alternative for these \u201cno-option\u201d patients, with many studies demonstrating the potential of stem cells to enhance revascularization by promoting vessel formation and blood flow recovery in ischemic tissues. Herein, we provide an overview of studies focused on the use of angiogenic cell therapies in CLI in the last years, from approaches testing different cell types in animal\/pre-clinical models of CLI, to the clinical trials currently under evaluation. Furthermore, recent alternatives related to stem cell therapies such as the use of secretomes, exosomes, or even microRNA, will be also described.<\/jats:p>","DOI":"10.3390\/ijms22052335","type":"journal-article","created":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T06:47:20Z","timestamp":1614322040000},"page":"2335","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Current Status of Angiogenic Cell Therapy and Related Strategies Applied in Critical Limb Ischemia"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4249-2886","authenticated-orcid":false,"given":"Luc\u00eda","family":"Beltr\u00e1n-Camacho","sequence":"first","affiliation":[{"name":"Biomedicine, Biotechnology and Public Health Department, C\u00e1diz University, 11519 Cadiz, Spain"},{"name":"Institute of Research and Innovation in Biomedical Sciences of Cadiz (INIBICA), 11009 C\u00e1diz, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Rojas-Torres","sequence":"additional","affiliation":[{"name":"Biomedicine, Biotechnology and Public Health Department, C\u00e1diz University, 11519 Cadiz, Spain"},{"name":"Institute of Research and Innovation in Biomedical Sciences of Cadiz (INIBICA), 11009 C\u00e1diz, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u1d43 Carmen","family":"Dur\u00e1n-Ruiz","sequence":"additional","affiliation":[{"name":"Biomedicine, Biotechnology and Public Health Department, C\u00e1diz University, 11519 Cadiz, Spain"},{"name":"Institute of Research and Innovation in Biomedical Sciences of Cadiz (INIBICA), 11009 C\u00e1diz, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.hlc.2017.10.014","article-title":"Peripheral Arterial Disease","volume":"27","author":"Conte","year":"2018","journal-title":"Heart Lung Circ."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/j.phrs.2016.09.040","article-title":"Prevalence and prevention of cardiovascular disease and diabetes mellitus","volume":"113","author":"Balakumar","year":"2016","journal-title":"Pharmacol. 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